[1]张立 董玮△ 董介轩 殷志荣 石小东 李皎 刘维统.针刀松解对坐骨神经痛大鼠中枢多巴胺、去甲肾上腺素及5-羟色胺的影响[J].中国中医骨伤科杂志,2020,28(08):13-16.
　ZHANG Li DONG Wei DONG Jiexuan YIN Zhirong SHI Xiaodong LI Jiao LIU Weitong.Effects of Acupotomy Release on Central DA, NE and 5-HT in Rats with Sciatica[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2020,28(08):13-16.
点击复制

针刀松解对坐骨神经痛大鼠中枢多巴胺、去甲肾上腺素及5-羟色胺的影响()
分享到：

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第28卷
期数:
2020年08期
页码:
13-16
栏目:
实验研究
出版日期:
2020-08-10

文章信息/Info

Title:
Effects of Acupotomy Release on Central DA, NE and 5-HT in Rats with Sciatica
文章编号:
1005-0205(2020)08-0013-04
作者:
张立1 董玮1△ 董介轩1 殷志荣1 石小东1 李皎1 刘维统1
1云南中医药大学第三附属医院(昆明市中医医院)骨伤科 (昆明,650032)
Author(s):
ZHANG Li1 DONG Wei1△ DONG Jiexuan1 YIN Zhirong1 SHI Xiaodong1 LI Jiao1 LIU Weitong1
1Department of Orthopedics and Traumatology,Kunming Hospital of Traditional Chinese Medicine,the Third Affiliated Hospital of Yunnan University of Traditional Chinese Medicine,Kunming 650032,China.
关键词:
坐骨神经痛;  针刀松解法;  中枢镇痛
Keywords:
sciatica;  acupotome release;  central analgesia
分类号:
R-33
文献标志码:
A
摘要:
目的:观察针刀松解法治疗坐骨神经痛大鼠对其延髓、丘脑、海马、腰膨大脊髓多巴胺(DA)、去甲肾上腺素(NE)、5-羟色胺(5-HT)及热缩足阈值的影响。方法:将60只SD大鼠随机分为假手术组、神经结扎组及针刀治疗组。假手术组仅手术暴露坐骨神经,不做结扎; 神经结扎组手术暴露并结扎坐骨神经; 针刀治疗组手术结扎坐骨神经,在造模成功后第7,14,21天分别进行针刀松解治疗。3组在第28天进行热缩足试验,测定大鼠热缩足阈值; 处死大鼠取延髓、丘脑、海马、腰膨大脊髓组织; 用ELISA法检测DA,5-HT及NE浓度。结果:针刀治疗组与假手术组对比,大鼠腰膨大脊髓、丘脑5-HT,NE,DA浓度高于假手术组,差异有统计学意义(P<0.01); 延髓5-HT,NE,DA浓度低于假手术组,差异有统计学意义(P<0.01)。针刀治疗组与神经结扎组对比,大鼠腰膨大脊髓5-HT,NE,DA浓度高于神经结扎组,延髓5-HT,NE,DA浓度低于神经结扎组,差异有统计学意义(P<0.01)。结论:针刀松解治疗坐骨神经痛大鼠能促进中枢DA,NE,5-HT在相应抑制疼痛受体分布区域的表达,起中枢镇痛的作用。
Abstract:
Objective:To observe the effects of acupotomatolysis on dopamine(DA),norepinephrine(NE),5-hydroxytryptamine(5-HT)and thermal foot threshold in sciatica rats.Methods:60 SD rats were randomly divided into sham operation group,nerve ligation group and acupotomology group.In the sham operation group,the sciatic nerve was exposed surgically without ligation.Nerve ligation group:rats accepted surgical exposure and ligation of the sciatic nerve.Acupotomology group:surgical ligation of sciatic nerve,acupotomology release treatment 7,14 and 21 d after successful modeling.The thermal foot shrinkage test was conducted in the three groups on 28 d to determine the threshold of thermal foot shrinkage in the rats.The rats were sacrificed and the medulla oblongata,thalamus,hippocampus and lumbar dilated spinal cord were collected.DA,5-HT and NE concentrations were determined by ELISA.Results:The concentrations of spinal cord enlargement of thalamus 5-HT,NE and DA in the acupotomology group were higher than those in the sham group(P<0.01).The concentrations of 5-HT,NE and DA in medulla oblongata were lower than those in the sham group(P<0.01).The concentrations of 5-HT,NE and DA in the lumbar enlargement spinal cord of acupotomology groupwere higher than those in the nerve ligation group,and the concentrations of 5-HT,NE and DA in the medulla oblanceolata were lower than those in the nerve ligation group(P<0.01).Conclusion:Acupotomy release therapy for sciatica rats can promote the expression of DA,NE and 5-HT in the corresponding pain receptor distribution area,and play the role of central analgesia.

参考文献/References:

[1] 崔艳红,张赛,陈旭义,等.坐骨神经痛的研究进展[J].中国医药,2019,14(7):1114-1117.
[2] 赵玲,陈少宗.针灸治疗坐骨神经痛的取穴处方决策与经验统计[J].针灸临床杂志,2011,27(10):46-48.
[3] HUNT S P,PINI A,EVAN G.Induction of c-fos like protein in spinal cordneurons following sensory stimulation[J].Nature,2008,328(6131):632-634.
[4] RUNU R,SINHA N K,PAI R,et al.Our experience with epidural steroid injections in management of low backpain and sciatica[J].Kathmandu University Medical Journal,2005,3(4):349-354.
[5] 韩易言,马铁明.针灸治疗坐骨神经痛荟萃分析[J].辽宁中医杂志,2017,44(7):1472-1476.
[6] 叶肖琳,黄雪莲,叶新苗.小针刀对腰椎间盘突出症治疗效果的Meta分析[J].中华中医药杂志,2016,31(7):2784-2788.
[7] BENNETT G J,XIE Y K.A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man[J].Pain,1988,33(1):87-107.
[8] ATTAL N,BARROT M.Is Pregabalin ineffective in acute or chronic sciatica[J].N Engl J Med,2017,376(12):1169-1170.
[9] 徐雪汝,林星武,傅少雄,等.脉冲射频背根神经节对CCI模型大鼠脊髓中Iba1和TRPV1表达的影响[J].中国疼痛医学杂志,2018,24(11):823-829.
[10] CHALLA S R.Surgical animal models of neuropathic pain:pros and cons[J].Int J Neurosci,2015,125(3):170-174.
[11] 肖智,文松.中脑导水管周围灰质5-HT7受体在神经病理性疼痛中的镇痛作用研究[J].中国疼痛医学杂志,2014,20(12):861-865.
[12] 刘利龙,王苹朱,熊俊瑶,等.5-HT系统在疼痛调节机制中的功能及其研究现状[J].南昌大学学报:医学版,2015,55(5):94-96.
[13] DOLY S,FISCHER J,BRISORGUEIL M J,et al.Pre-and postsynaptic localization of the 5-HT7 receptor in rat dorsal spinal cord:immunocytochemical evidence[J].J Comp Neurol,2005,490:256-269.
[14] MARTIN-CORA F J,PAROS A.Autoradiographic distribution of 5-HT7 receptors in the human brain using[3H]mesulergine:comparison to other mammalian species[J].British Journal of Pharmacology and Chemotherapy,2004,141(1):92-104.
[15] 龚晓康,乐凯,皮明山,等.左旋四氢帕马丁在治疗甲基苯丙胺成瘾中的应用研究进展[J].神经损伤与功能重建,2015,10(1):65-67.
[16] 宋雨田,杨清湖,刘霞,等.脊髓多巴胺D2受体对疼痛的调控[J].延安大学学报:医学科学版,2015,13(1):51-53.
[17] 李先林,王静,王雪颖.阿片成瘾的神经生物学机制研究进展[J].临床医药文献电子杂志,2017,33(4):6524-6526.
[18] ROH D H,KIM H W,YOON S Y,et al.Intrathecal clonidine suppresses phosphorylation of the N-methyl-daspartate receptor NRI subunit in spinal dorsal horn neurons of rats with neuropathic pain[J].Anesth Analg,2008,107(2):693-700.
[19] WU L J,ZHUO M.Targeting the NMDA receptor subunit NR2B for the treatment of neuropathic pain[J].Neurotherapeutics,2009,6(4):693-702.

备注/Memo

备注/Memo:
(收稿日期:2019-12-03)基金项目:云南省医疗卫生单位内设研究机构科研项目 (2016NS103) 2017云南省科技计划项目(2017FF117-(-048)) △通信作者 E-mail:dongwei310@126.com
更新日期/Last Update: 2020-08-10