[1]张少娥 王啸 谭红略△.解毒化瘀方对金黄色葡萄球菌黏附及生物膜形成的影响[J].中国中医骨伤科杂志,2020,28(08):8-12.
　ZHANG Shaoe WANG Xiao TAN Honglue.Inhibitory Effect of Jiedu Huayu Decoction on the Adhesion and Biofilm Formation of Staphylococcus Aureus[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2020,28(08):8-12.
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解毒化瘀方对金黄色葡萄球菌黏附及生物膜形成的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第28卷
期数:
2020年08期
页码:
8-12
栏目:
实验研究
出版日期:
2020-08-10

文章信息/Info

Title:
Inhibitory Effect of Jiedu Huayu Decoction on the Adhesion and Biofilm Formation of Staphylococcus Aureus
文章编号:
1005-0205(2020)08-0008-05
作者:
张少娥1 王啸1 谭红略1△
1河南省正骨研究院,河南省洛阳正骨医院(河南省骨科医院) (河南 洛阳,471000)
Author(s):
ZHANG Shaoe1 WANG Xiao1 TAN Honglue1△
1Henan Orthopedic Institute,Henan Luoyang Orthopedic-Traumatological Hospital(Henan Orthopedic Hospital), Luoyang 471000,Henan China.
关键词:
解毒化瘀方;  金黄色葡萄球菌;  黏附;  细菌生物膜
Keywords:
Jiedu Huayu decoction;  multidrug-resistant staphylococcus aureus;  adhesion;  bacterial biofilm
分类号:
R-33
文献标志码:
A
摘要:
目的:观察解毒化瘀方对钛表面金黄色葡萄球菌黏附及生物膜形成的抑制效用。方法:以耐药金黄色葡萄球菌ATCC43300为研究对象,以解毒化瘀方TSB(Tryptic Soy Broth)不同稀释浓度或与细菌孵育不同时间作为观察组(TSB-J组)分组依据,以单纯TSB培养基(TSB组)及含庆大霉素TSB(TSB-G组)与细菌孵育作为对照组。通过结晶紫染色、细菌超声震荡涂板计数、激光共聚焦显微镜(CLSM)及扫描电镜(SEM)等,观察不同处理因素对钛表面细菌黏附及生物膜形成的抑制效果及特点。结果:无论是定量还是定性分析,相对于TSB及TSB-G对照组,解毒化瘀方处理组可明显抑制钛表面ATCC43300菌株的黏附及生物膜形成,且存在随着解毒化瘀方浓度或孵育时间的增加,其抑制效果更明显的趋势。结论:解毒化瘀方可抑制葡萄球菌,尤其是耐药菌在钛表面的黏附及生物膜的形成,其效用与解毒化瘀方浓度或孵育时间存在依赖关系。
Abstract:
Objective:To investigate the inhibitory effect of jiedu huayu decoction on the adhesion and biofilm formation of staphylococcus aureus on the titanium surface.Methods:Multidrug-resistant Staphylococcus aureus ATCC43300 were used in this study.The different dilution concentration of Jiedu Huayu decoction(J)with TSB(Tryptic Soy Broth)or incubation time of Jiedu Huayu decoction with bacteria were used as the grouping basis for experimental group(TSB-J group).The pure TSB culture medium(TSB group)and TSB containing gentamicin(TSB-G group)incubating with bacteria were designed as the control groups.The inhibitory effect and characteristics of different treatment process on bacterial adhesion and biofilm formation on the titanium surface were observed using crystal violet staining,bacterial ultrasonic plus the spread plate method,CLSM(Confocal Laser Scanning microscopy)and SEM(Scanning Electron Microscopy).Results:In both quantitative or qualitative analysis,as compared with TSB and TSB-G group,Jiedu Huayu groups could significantly inhibited the adhesion and biofilm formation of ATCC43300 on the titanium surface.Moreover,with the increase of concentration or incubation time of Jiedu Huayu decoction,the inhibition effect became more obvious.Conclusion:Jiedu Huayu decoction can inhibit the adhesion and biofilm formation of drug-resistant bacteria on the titanium surface,its effect is dependent on the concentration or incubation time of Jiedu Huayu decoction.

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备注/Memo

备注/Memo:
(收稿日期:2020-02-08)基金项目:NSFC-河南联合基金(U1804189) 河南省科技攻关项目(162102310369) △通信作者 E-mail:hnlc.love@163.com
更新日期/Last Update: 2020-08-10