[1]李金生 席刚△ 张志伟 张福杰.盘龙七片抑制老年大鼠髋部骨折后炎性反应的机制研究[J].中国中医骨伤科杂志,2020,28(08):17-20.
　LI Jinsheng XI Gang ZHANG Zhiwei ZHANG Fujie.Research on the Mechanism of Panlongqi Tablet Inhibiting Inflammation after Hip Fracture in Aged Rats[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2020,28(08):17-20.
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盘龙七片抑制老年大鼠髋部骨折后炎性反应的机制研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第28卷
期数:
2020年08期
页码:
17-20
栏目:
实验研究
出版日期:
2020-08-10

文章信息/Info

Title:
Research on the Mechanism of Panlongqi Tablet Inhibiting Inflammation after Hip Fracture in Aged Rats
文章编号:
1005-0205(2020)08-0017-04
作者:
李金生1 席刚1△ 张志伟1 张福杰1
1山西医科大学第二医院(太原,030001)
Author(s):
LI Jinsheng1 XI Gang1△ ZHANG Zhiwei1 ZHANG Fujie1
1Second Hospital of Shanxi Medical University,Taiyuan 030001,China.
关键词:
盘龙七片;  髋部骨折;  大鼠模型;  细胞因子;  炎性反应
Keywords:
Panlongqi tablet;  hip fracture;  rat model;  cytokines;  inflammation
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨中成药盘龙七片对髋部骨折后炎性损伤的保护作用及机理。方法:采用左侧髋部骨折法制备髋部骨折老年大鼠模型,灌胃给药盘龙七片后检测模型鼠血清及体外巨噬细胞、单核细胞中炎性因子IL-6,TGF-β,IL-1β,IL-10,BAFF的表达变化及Th17细胞应答的变化。结果:盘龙七片组模型鼠血清中及体外巨噬细胞、单核细胞中炎性因子IL-6,TGF-β,IL-1β,IL-17,BAFF水平均显著降低,而抑炎因子IL-10水平则明显升高,差异有统计学意义(P<0.05); 同时,盘龙七片对促Th17因子的抑制作用则可被rBAFF削弱,而被BAFF单抗处理加强,差异有统计学意义(P<0.05)。结论:盘龙七片可通过抑制BAFF激活的Th17炎性反应进而发挥对髋部骨折后的治疗作用。
Abstract:
Objective:To investigate the protective mechanism of Panlongqi tablet on aged rats with hip fracture.Methods:The rat model was established by left hip fracture method.After intragastric administration of Panlongqi tablet,the expression changes of inflammatory factors IL-6,TGF-β,IL-1β,IL-10,BAFF as well as the Th17 cell response were tested fromserum in vivo as well as macrophages and monocytes in vitro.Results:After administrated with Panlongqi tablet,the levels of inflammatory factors IL-6,TGF-β,IL-1β,IL-10 and BAFF in serum as well as macrophages and monocytes were significantly reduced,while the levels of anti-inflammatory factor IL-10 were significantly increased(P<0.05).Meanwhile,the inhibitory effect of Panlongqi tablet on pro-Th17 factor was weakened by rBAFF and strengthened by BAFF monoclonal antibody(P<0.05).Conclusion:Panlongqi tablet play protective role in hip fracture by reducing the inflammatory response via inhibiting BAFF-activated Th17 response.

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备注/Memo

备注/Memo:
(收稿日期:2020-03-22) △通信作者 E-mail:1003007294@qq.com
更新日期/Last Update: 2020-08-10