[1]王志力,胡兴律,殷继超△,等.理筋手法影响竖脊肌脂肪浸润的机制研究[J].中国中医骨伤科杂志,2023,31(04):15-19+24.[doi:10.20085/j.cnki.issn1005-0205.230403 ]
　WANG Zhili,HU Xingl,YIN Jichao,et al.Mechanism Study of Tendon Manipulation in Regulating Fat Infiltration in Erector Spinal Muscles[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2023,31(04):15-19+24.[doi:10.20085/j.cnki.issn1005-0205.230403 ]
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理筋手法影响竖脊肌脂肪浸润的机制研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第31卷
期数:
2023年04期
页码:
15-19+24
栏目:
实验研究
出版日期:
2023-04-15

文章信息/Info

Title:
Mechanism Study of Tendon Manipulation in Regulating Fat Infiltration in Erector Spinal Muscles
文章编号:
1005-0205(2023)04-0015-05
作者:
王志力1; 胡兴律2; 殷继超1; 3△; 程渊1; 2; 王磊磊1; 2; 徐周1; 2
1陕西中医药大学(陕西 咸阳,712046) 2西安市中医院 3西安市卫生学校
Author(s):
WANG Zhili1; HU Xinglü2; YIN Jichao1; 3△; CHENG Yuan1; 2; WANG Leilei1; 2; XU Zhou1; 2
1Shaanxi University of Chinese Medicine,Xianyang 712046,Shaanxi China; 2Xi'an Hospital of Traditional Chinese Medicine,Xian 710021,China; 3Xi'an Health School,Xi'an 710054,China.
关键词:
竖脊肌;  脂肪浸润;  炎症;  通路;  理筋手法
Keywords:
erector spinal muscles;  fat infiltration;  inflammation;  pathway;  tendon manipulation
分类号:
R-33
DOI:
10.20085/j.cnki.issn1005-0205.230403
文献标志码:
A
摘要:
目的:观察理筋手法经β-catenin/PPARγ信号通路对大白兔竖脊肌脂肪浸润的影响,分析其作用机制。方法:将18只新西兰大白兔随机分为空白组、模型组及治疗组,每组6只,2 d治疗1次,连续6周。竖脊肌采用苏木精-伊红(HE)染色观察病理学变化,酶联免疫吸附测定(ELISA)法检测大白兔血清中白细胞介素-1(IL-1)、肿瘤坏死因子-α(TNF-α)及脂肪因子瘦素(Leptin)、脂联素(Adiponectin,ADPN)的水平,蛋白免疫印迹法(Western Blot)检测竖脊肌组织中β-连环蛋白(β-catenin)、过氧化物酶体增殖物激活受体γ(PPARγ)蛋白表达水平。结果:HE染色结果显示:与空白组相比,模型组肌纤维数量减少、排列紊乱,肌束间隔增大,肌束内及肌束间脂肪细胞簇集增多,体积增大,理筋手法治疗组肌束间隔小于模型组,脂肪细胞数量及体积也小于模型组。ELISA法检测结果:与空白组相比,模型组大白兔血清Leptin、TNF-α水平明显升高,差异有统计学意义(P<0.05); IL-1水平显著升高,差异有统计学意义(P<0.01),而ADPN水平明显降低,差异有统计学意义(P<0.01)。与模型组相比,治疗组血清TNF-α、IL-1及Leptin水平明显降低,差异有统计学意义(P<0.05),而ADPN水平显著升高,差异有统计学意义(P<0.05)。Western Blot检测结果:与空白组相比,模型组中β-catenin蛋白显著降低,差异有统计学意义(P<0.01); 与模型组相比,治疗组显著升高,差异有统计学意义(P<0.001); 与空白组相比,模型组中PPARγ蛋白明显升高,差异有统计学意义(P<0.01); 与模型组相比,治疗组中PPARγ蛋白显著降低,差异有统计学意义(P<0.01)。结论:理筋手法改善竖脊肌脂肪浸润,可能与调控β-catenin/PPARγ信号通路、调节炎症因子及脂肪因子水平有关。
Abstract:
Objective:To observe the effect of tendon manipulation on the β-catenin/PPARγ signaling pathway in the muscle tissue of erector spinal muscle fat infiltration in white rabbit,and to analyze its mechanism of action.Methods:18 New Zealand white rabbits were randomly divided into blank group,model group and treatment group,with 6 rabbits in each group,and the treatment was once every other day for 6 weeks.Hematoxylin-eosin(HE)staining of erector spinal muscles was used to observe pathological changes, the levels of interleukin-1(IL-1),tumor necrosis factor-α(TNF-α),leptin and adiponectin(ADPN)in serum of white rabbits were detected by Enzyme-linked immunosorbent assay(ELISA).Western Blot was used to detect the expression levels of β-catenin and peroxisome proliferator-activated receptor γ(PPARγ)proteins in erector spinal muscle tissue.Results: Compared with the blank group,the number of myofibers in the model group was reduced, the arrangement was disordered, the fascicular septum increased, the cluster of adipocytes in and between the fasciculations increased,and the volume increased, the fascicular spacing in the tendon manipulation treatment group was smaller than that in the model group,and the number and volume of adipocytes were also smaller than those in the model group.ELISA test results:compared with the blank group,the serum leptin and TNF-α levels in the model group were significantly increased(P<0.05), IL-1 levels were significantly increased(P<0.01),while the ADPN levels were significantly reduced(P<0.01).Compared with the model group,the serum TNF-α,IL-1 and leptin levels in the treatment group were significantly lower(P<0.05),while the ADPN levels were significantly increased(P<0.05).Western Blot detection results: compared with the blank group,the β-catenin protein in the model group was significantly reduced(P<0.01), and compared with the model group,the β-catenin protein in the treatment group significantly increased(P<0.001).Compared with the blank group,the PPARγ protein in the model group was significantly increased(P<0.01),and compared with the model group,the PPARγ protein in the treatment group was significantly reduced(P<0.01).Conclusion:The improvement of fat infiltration in erector spinal muscles by tendon manipulation may be related to the regulation of β-catenin/PPARγ signaling pathway and the regulation of inflammatory factors and adipose factor levels.

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备注/Memo

备注/Memo:
基金项目:陕西省中医药管理局项目(2021-ZZ-JC038)
陕西省科技厅一般项目(2021SF-372)
△通信作者 E-mail:444604786@qq.com
更新日期/Last Update: 2023-04-10