[1]王亚男,李艳,武文印,等.蒲公英提取物对类风湿性关节炎成纤维样滑膜细胞凋亡及炎症因子的影响[J].中国中医骨伤科杂志,2023,31(04):9-14.[doi:10.20085/j.cnki.issn1005-0205.230402 ]
　WANG Yanan,LI Yan,WU Wenyin,et al.Efficacy of Dandelion Extract on Apoptosis and Inflammatory Factors of Fibroblast-Like Synovial Cells in Rheumatoid Arthritis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2023,31(04):9-14.[doi:10.20085/j.cnki.issn1005-0205.230402 ]
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蒲公英提取物对类风湿性关节炎成纤维样滑膜细胞凋亡及炎症因子的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第31卷
期数:
2023年04期
页码:
9-14
栏目:
实验研究
出版日期:
2023-04-15

文章信息/Info

Title:
Efficacy of Dandelion Extract on Apoptosis and Inflammatory Factors of Fibroblast-Like Synovial Cells in Rheumatoid Arthritis
文章编号:
1005-0205(2023)04-0009-06
作者:
王亚男1; 李艳2; 武文印2; 王子1△
1唐山市中医医院风湿免疫代谢病区(河北 唐山,063000) 2唐山市中医医院治未病科
Author(s):
WANG Yanan1; LI Yan2; WU Wenyin2; WANG Zi1△
1Rheumatism Immunometabolism Ward,Tangshan Hospital of Traditional Chinese Medicine,Tangshan 063000,Hebei China; 2Department of Preventive Medicine,Tangshan Hospital of Traditional Chinese Medicine,Tangshan 063000,Hebei China.
关键词:
类风湿性关节炎;  成纤维样滑膜细胞;  蒲公英提取物;  细胞增殖;  细胞凋亡
Keywords:
rheumatoid arthritis;  fibroblast-like synovial cells;  dandelion extract;  cell proliferation;  apoptosis
分类号:
R684.3
DOI:
10.20085/j.cnki.issn1005-0205.230402
文献标志码:
A
摘要:
目的:探讨蒲公英提取物对类风湿性关节炎成纤维样滑膜(FLS)细胞增殖、凋亡及炎症的影响及其可能作用机制。方法:原代分离培养类风湿性关节炎成纤维样滑膜细胞,不同浓度的蒲公英提取物处理成纤维样滑膜细胞,检测细胞增殖、凋亡及IL-6、TNF-α; qRT-PCR法检测miR-708-5p的表达量; miR-NC、miR-708-5p mimics分别转染至成纤维样滑膜细胞,anti-miR-NC、anti-miR-708-5p分别转染至成纤维样滑膜细胞后加入5 mg/mL蒲公英提取物培养细胞,检测细胞增殖、凋亡及IL-6、TNF-α。结果:蒲公英提取物处理后细胞活力和IL-6、TNF-α的水平降低(P<0.05),miR-708-5p的表达量、细胞凋亡率升高(P<0.05); 转染miR-708-5p mimics可降低细胞活力和IL-6、TNF-α的水平(P<0.05),细胞凋亡率升高(P<0.05); 转染anti-miR-708-5p可减弱蒲公英提取物对成纤维样滑膜细胞增殖、凋亡及炎症反应的作用。结论:蒲公英提取物可通过促进miR-708-5p表达而抑制类风湿性关节炎成纤维样滑膜细胞增殖、炎症反应并诱导细胞凋亡。
Abstract:
Objective:To explore the efficacy of dandelion extract on the proliferation,apoptosis and inflammation of fibroblast-like synovial cells in rheumatoid arthritis and its possible mechanism.Methods:Primary isolation and culture of rheumatoid arthritis fibroblast-like synovial(FLS)cells was performed,and the FLS cells were treated with different concentrations of dandelion extract.The cell proliferation,apoptosis and IL-6 and TNF-α were detected.The qRT-PCR method was used to detect the expression of miR-708-5p.The miR-NC and miR-708-5p mimics were transfected into FLS cells; anti-miR-NC and anti-miR-708-5p were transfected into FLS cells respectively,and 5 mg/mL dandelion extract was added to culture cells.The above methods were used to detect cell proliferation,apoptosis and the levels of IL-6 and TNF-α.Results:After dandelion extract treatment,cell viability and the levels of IL-6,TNF-α were decreased(P<0.05),while the expression of miR-708-5p,cell apoptosis rate were increased(P<0.05).Transfection of miR-708-5p mimics could reduce cell viability and the levels of IL-6,TNF-α(P<0.05),while cell apoptosis was increased(P<0.05).Transfection of anti-miR-708-5p could attenuate the effects of dandelion extract on the proliferation,apoptosis and inflammation of FLS cells.Conclusion:Dandelion extract could inhibit rheumatoid arthritis fibroblast-like synovial cell proliferation,inflammation and induce cell apoptosis by promoting the expression of miR-708-5p.

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备注/Memo

备注/Memo:
基金项目:河北省中医药管理局科研计划项目(2017129)
△通信作者 E-mail:1694936134@qq.com
更新日期/Last Update: 2023-04-10