[1]李明洋,冯帅华,蒋佳豪,等.补肾活血汤调控炎症小体改善大鼠椎间盘退变的实验研究[J].中国中医骨伤科杂志,2023,31(01):1-6.
　LI Mingyang,FENG Shuaihua,JIANG Jiahao,et al.Experimental Study on Bushen Huoxue Decoction Regulating Inflammasome in Improving Intervertebral Disc Degeneration in Rats[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2023,31(01):1-6.
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补肾活血汤调控炎症小体改善大鼠椎间盘退变的实验研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第31卷
期数:
2023年01期
页码:
1-6
栏目:
实验研究
出版日期:
2023-01-15

文章信息/Info

Title:
Experimental Study on Bushen Huoxue Decoction Regulating Inflammasome in Improving Intervertebral Disc Degeneration in Rats
文章编号:
1005-0205(2023)01-0001-06
作者:
李明洋1; 冯帅华2; 蒋佳豪1; 李泽湘1; 王镇远1; 罗阳骞1; 杨届2△; 吴官保2△
1湖南中医药大学(长沙, 410208) 2湖南省中医药研究院附属医院
Author(s):
LI Mingyang1; FENG Shuaihua2; JIANG Jiahao1; LI Zexiang1; WANG Zhenyuan1; LUO Yangqian1; YANG Jie2△; WU Guanbao2△
1Hunan University of Chinese Medicine,Changsha 410208,China; 2Affiliated Hospital of Hunan Academy of Chinese Medicine,Changsha 410006,China.
关键词:
补肾活血汤;  炎症小体;  椎间盘退变
Keywords:
Bushen Huoxue decoction;  inflammasome;  intervertebral disc degeneration
分类号:
R-33
文献标志码:
A
摘要:
目的:观察补肾活血汤对退变椎间盘中NLRP3/Caspase-1炎症小体的影响并探究补肾活血汤改善腰椎间盘退变的作用机制。方法:SD大鼠70只(雌雄各半),随机分为3组:Ⅰ组50只进行椎间盘退变模型建立,Ⅱ组10只作为空白组(A组),Ⅲ组10只作为假手术组(B组)。验证造模成功后,Ⅰ组取44只大鼠随机分为模型组8只(C组)、抑制剂组18只(D组,Caspase-1抑制剂灌胃)及中药组18只(E组,补肾活血汤灌胃)。各组于喂养2周后随机处死组内半数大鼠取出其L3/4至L5/6椎间盘组织,4周后将剩余大鼠全部处死取L3/4至L5/6椎间盘组织,样本行苏木精-伊红(HE)染色,Western Blot法检测NLRP3、Caspase-1蛋白表达水平,PCR检测NLRP3、Caspase-1基因表达水平,ELISA法测定IL-1β、TNF-α蛋白含量。结果:与空白组比较,模型组NLRP3、Caspase-1、IL-1β、TNF-α各项指标均显著高于空白组,差异有统计学意义(P<0.01); 与模型组比较,中药组NLRP3、Caspase-1、IL-1β、TNF-α各项指标均显著低于模型组,差异有统计学意义(P<0.01)。结论:补肾活血汤可以通过抑制椎间盘内NLRP3/Caspase-1炎症小体起到治疗或延缓椎间盘退变的作用。
Abstract:
Objective:To observe the efficacy of Bushen Huoxue decoction on NLRP3/Caspase-1 inflammasome in degenerative intervertebral discs and explore the mechanism of Bushen Huoxue decoction in improving lumbar intervertebral disc degeneration.Methods:70 SD rats(half male and half female)were randomly divided into three groups,50 rats in group Ⅰ were used to establish the intervertebral disc degeneration model,10 rats in group Ⅱ were used as the blank group(group A),and 10 rats in group Ⅲ were used as the sham operation group(group B).After verifying the successful modeling,44 rats in group Ⅰ were randomly divided into model group(group C,n=8),inhibitor group(group D,Caspase-1 inhibitor gavage,n=18),and traditional Chinese medicine group(group E,Bushen Huoxue decoction gavage,n=18).After feeding for 2 weeks,half of the rats in each group were killed,and their L3/4 to L5/6 intervertebral disc tissues were taken out.After 4 weeks,all the remaining rats were killed,and the L3/4 to L5/6 intervertebral disc tissues were taken out.The samples were stained with HE,the expression levels of NLRP3 and Caspase-1 proteins were detected by Western Blot,the expression levels of NLRP3 and Caspase-1 genes were detected by PCR,and the protein contents of IL-1β,TNF-α were detected by ELISA.Results:Compared with the blank group,all the indexes of NLRP3,Caspase-1,IL-1β,TNF-α in the model group were significantly higher than those in the blank group(P<0.01).Compared with the model group,all the indexes of NLRP3,Caspase-1,IL-1β,TNF-α in the traditional Chinese medicine group were significantly lower than those in the model group(P<0.01).Conclusion:Bushen Huoxue decoction can treat or delay intervertebral disc degeneration by inhibiting NLRP3/Caspase-1 inflammasome in the intervertebral disc.

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备注/Memo

备注/Memo:
基金项目:湖南省自然科学基金面上项目(2022JJ30025)
长沙市自然科学基金面上项目(kq2202475)
湖南省中医药科研计划项目(2021094)
湖南省中医药研究院联合基金重点项目(202016)
湖南省中医药科研计划项目(201902)
△通信作者 E-mail:593557170@qq.com(杨届)
更新日期/Last Update: 2023-01-10