[1]艾力江·阿斯拉,梁治权,李雷疆,等.补肾生骨汤对股骨头无菌性坏死大鼠的影响[J].中国中医骨伤科杂志,2023,31(01):7-12.
　Ailijiang Asila,LIANG Zhiquan,LI Leijiang,et al.Efficacy of Bushen Shenggu Decoction on miR-135b-5p/RUNX2 Signaling Axis in Rats with Aseptic Necrosis of the Femoral Head[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2023,31(01):7-12.
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补肾生骨汤对股骨头无菌性坏死大鼠的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第31卷
期数:
2023年01期
页码:
7-12
栏目:
实验研究
出版日期:
2023-01-15

文章信息/Info

Title:
Efficacy of Bushen Shenggu Decoction on miR-135b-5p/RUNX2 Signaling Axis in Rats with Aseptic Necrosis of the Femoral Head
文章编号:
1005-0205(2023)01-0007-06
作者:
艾力江·阿斯拉1; 梁治权2; 李雷疆2; 邓迎杰2; 廖军2△; 王继先2
1新疆维吾尔自治区中医药研究院(乌鲁木齐,830000) 2新疆医科大学附属中医医院骨科
Author(s):
Ailijiang Asila1; LIANG Zhiquan2; LI Leijiang2; DENG Yingjie2; LIAO Jun2△; WANG Jixian2
1Xinjiang Uygur Autonomous Region Institute of Traditional Chinese Medicine,Urumqi 830000,China; 2Department of Orthopedics,Affiliated Hospital of Traditional Chinese Medicine,Xinjiang Medical University,Urumqi 830000,China.
关键词:
补肾生骨汤;  股骨头无菌性坏死;  骨钙素;  血管内皮生长因子;  一氧化氮合成酶
Keywords:
Bushen Shenggu decoction;  osteonecrosis of the femoral head;  bone glaprotein;  vascular endothelial growth factor;  nitric oxide synthase
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨补肾生骨汤调节miR-135b-5p/Runt相关基因2(RUNX2)信号轴对股骨头无菌性坏死(ONFH)大鼠的影响。方法:将SD大鼠随机分为假手术(Sham)组、ONFH组、低/中/高剂量补肾生骨汤(0.13,0.26,0.52 g/mL,L/M/H-补肾生骨汤)组、H-补肾生骨汤+miR-135b-5p agomir组、H-补肾生骨汤+agomir-NC组,采用液氮冷冻法制备ONFH大鼠模型; 苏木精-伊红(HE)染色观察股骨头组织病理改变; 检测血浆中纤维蛋白原(FIB)、血管内皮生长因子(VEGF)、一氧化氮合成酶(NOS)、骨钙素(BGP)水平; RT-qPCR仪、Western Blot法分别检测股骨头组织中miR-135b-5p、RUNX2表达水平; 双荧光素酶实验分析miR-135b-5p与RUNX2的靶向关系。结果:与Sham组比较,ONFH组股骨头组织发生区域性缺损,骨小梁稀疏、行走不规则,空骨陷窝增多明显; 血浆中FIB表达升高,VEGF、NOS、BGP表达降低(P<0.05); 经L/M/H-补肾生骨汤干预后,上述情况均改善,且经H-补肾生骨汤干预改善更明显; 在H-补肾生骨汤干预的基础上增加miR-135b-5p agomir干预后,H-补肾生骨汤干预的改善作用被削弱。与Sham组比较,ONFH组股骨头组织中miR-135b-5p表达升高,RUNX2表达降低(P<0.05); 经H-补肾生骨汤干预后,miR-135b-5p表达降低,RUNX2表达升高(P<0.05); 在H-补肾生骨汤干预的基础上增加miR-135b-5p agomir干预后,miR-135b-5p表达升高,RUNX2表达降低(P<0.05)。双荧光素酶实验结果显示,miR-135b-5p与RUNX2存在靶向关系。结论:补肾生骨汤能促进ONFH大鼠股骨头组织修复,可能与下调miR-135b-5p进而靶向上调RUNX2有关。
Abstract:
Objective:To investigate the efficacy of Bushen Shenggu decoction on microRNA(miR)-135b-5p/Runt-related transcription factor 2(RUNX2)signaling axis in rats with aseptic necrosis of the femoral head(ONFH).Methods:SD rats were randomly divided into Sham operation group,ONFH group,low/medium/high-dose Bushen Shenggu decoction(0.13,0.26,0.52 g/mL)groups,H-Bushen Shenggu decoction + miR-135b-5p agomir group,and H-Bushen Shenggu decoction + agomir-NC group.The ONFH rat model was established by liquid nitrogen freezing method.Histopathological changes of femoral head were observed by hematoxylin-eosin(HE)staining.The levels of fibrinogen(FIB),vascular endothelial growth factor(VEGF),nitric oxide synthase(NOS)and osteocalcin(BGP)in plasma were detected.RT-qPCR and Western Blot were performed to detect the expression levels of miR-135b-5p and RUNX2 in femoral head tissue,respectively.The targeting relationship between miR-135b-5p and RUNX2 was analyzed by dual luciferase experiments.Results:Compared with the Sham group,the femoral head tissue in the ONFH group had regional defects,sparse and irregular trabecular bone,and greatly more empty bone lacunae; the expression of FIB in plasma increased,while the expression of VEGF,NOS and BGP decreased(P<0.05).After the intervention of L/M/H-Bushen Shenggu decoction,the above conditions were improved,and the improvement was more obvious after the intervention of H-Bushen Shenggu decoction.After adding miR-135b-5p agomir on the basis of H-Bushen Shenggu decoction intervention,the improvement efficacy of H-Bushen Shenggu decoction intervention was weakened.Compared with the Sham group,the expression of miR-135b-5p in the femoral head tissue of the ONFH group increased,and the expression of RUNX2 decreased(P<0.05).After the intervention of H-Bushen Shenggu decoction,the expression of miR-135b-5p decreased and the expression of RUNX2 increased(P<0.05),the expression of miR-135b-5p increased and the expression of RUNX2 decreased after agomir intervention on the basis of H-Bushen Shenggu decoction(P<0.05).The results of dual luciferase experiments showed that miR-135b-5p had a targeting relationship with RUNX2.Conclusion:Bushen Shenggu decoction can promote the repair of femoral head tissue in ONFH rats,which may be related to the down-regulation of miR-135b-5p and the targeted up-regulation of RUNX2.

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备注/Memo

备注/Memo:
基金项目:新疆维吾尔自治区自然科学基金资助项目(2019D01C178)
△通信作者 E-mail:devil1234543@163.com
更新日期/Last Update: 2023-01-10