[1]李硕祺,夏铎,陈铁柱,等.盘龙七片调节骨保护素/核因子κB受体活化因子配体信号通路对去卵巢骨质疏松大鼠的影响[J].中国中医骨伤科杂志,2022,30(01):10-14+18.
　LI Shuoqi,XIA Duo,CHEN Tiezhu,et al.Study of Efficacy of Panlongqi Tablet Modulated OPG/RANKL Pathway in Ovariectomized Rats with Osteoporosis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2022,30(01):10-14+18.
点击复制

盘龙七片调节骨保护素/核因子κB受体活化因子配体信号通路对去卵巢骨质疏松大鼠的影响()
分享到：

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第30卷
期数:
2022年01期
页码:
10-14+18
栏目:
实验研究
出版日期:
2022-01-15

文章信息/Info

Title:
Study of Efficacy of Panlongqi Tablet Modulated OPG/RANKL Pathway in Ovariectomized Rats with Osteoporosis
文章编号:
1005-0205(2022)01-0010-05
作者:
李硕祺1; 夏铎1; 陈铁柱1; 孟建国2; 李晓声1△
1湖南省人民医院骨关节运动医学科(长沙,410001) 2陕西盘龙药业集团股份有限公司
Author(s):
LI Shuoqi1; XIA Duo1; CHEN Tiezhu1; MENG Jianguo2; LI Xiaosheng1△
1Department of Bone and Joint Sports Medicine,Hunan Provincial People's Hospital,Changsha 410001,China; 2Shaanxi Panlong Pharmaceutical Group Co.,Xi'an 710025,China.
关键词:
盘龙七片;  骨质疏松;  骨保护素;  核因子-κB受体活化因子配体
Keywords:
Panlongqi tablet;  osteoporosis;  osteoprotegerin;  receptor activator of nuclear factor-κB ligand
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨盘龙七片对去卵巢骨质疏松大鼠骨保护素(OPG)/核因子κB受体活化因子配体(RANKL)信号通路的影响。方法:75只雌性SD大鼠随机分为假手术组、模型组、阳性药物对照组(17β-雌二醇,1 mg/kg)、盘龙七片低剂量组(0.26 g/kg)、盘龙七片高剂量组(1.29 g/kg),每组15只,除假手术组外,其余各组大鼠建立去卵巢骨质疏松模型,分组给予相应药物处理后,苏木精-伊红(HE)染色法染色观察各组大鼠股骨并成像; 通过双能X线骨密度分析仪对大鼠离体股骨密度进行检测; ELISA测定血清β-胶原降解产物(β-CTX)和骨碱性磷酸酶(BALP)水平; Western Blot测定左侧胫骨OPG、RANKL蛋白相对表达水平。结果:模型组左侧股骨远端骨小梁稀疏、结构不完整、明显变薄、排列紊乱; 盘龙七片低剂量组左侧股骨远端骨小梁有所改善,盘龙七片高剂量组与阳性药物组左侧股骨远端骨小梁形态结构明显改善。与假手术组相比,模型组大鼠BMC、最大载荷与弹性模量、BALP水平及OPG蛋白表达水平显著降低,差异有统计学意义(P<0.05),血清中β-CTX水平及RANKL蛋白水平显著升高,差异有统计学意义(P<0.05); 与模型组相比,盘龙七片低、高剂量组及阳性药物组大鼠BMC、最大载荷与弹性模量、BALP水平及OPG蛋白表达水平显著升高,差异有统计学意义(P<0.05),血清中β-CTX水平及RANKL蛋白水平显著降低,差异有统计学意义(P<0.05)。结论:盘龙七片可能通过抑制RANKL水平,上调OPG表达,改善去卵巢大鼠的骨质疏松症状。
Abstract:
Objective:To investigate the efficacy of Panlongqi tablet on osteoprotegerin(OPG)/receptor activator of nuclear factor-κB ligand(RANKL)signaling pathway in ovariectomized osteoporosis rats.Methods:A total of 75 female SD rats were randomly divided into sham operation group,model group,positive drug control group(17 β-estradiol,1 mg/kg),Panlongqi tablet low-dose group(0.26 g/kg)and Panlongqi tablet high-dose group(1.29 g/kg),with 15 rats in each group.Excepting the sham operation group,ovariectomized osteoporosis models were established in other groups.After treatment with corresponding drugs in groups,the femurs were observed and imaged by Hematoxylin Eosin staining(HE).The density of femur was detected by dual energy X-ray absorptiometry.The expressions of β-cross-linked c-telopeptide of type Ⅰ collagen(β-CTX)and bone alkaline phosphatase(BALP)in serum were detected by ELISA,and the related expression level of OPG/RANKL protein in left tibia was measured by Western Blot.Results:The trabecular bones of the distal femur on the left side in the model group were sparse,structurally incomplete,obviously thinned and disorderly arranged.The trabecular bone of the left distal femur in the low-dose tablet group was improved,and the trabecular bone morphology and structure of the left distal femur in the high-dose Panlongqi tablet group and the positive drug group were significantly improved.Compared with the sham operation group,BMC,maximum load and elastic modulus,BALP levels and OPG protein expression levels in the model group were significantly reduced(P<0.05),and β-CTX levels and RANKL protein levels in serum were significantly increased(P<0.05).Compared with the model group,the BMC,maximum load and elastic modulus,BALP level and OPG protein expression level of Panlongqi tablet low,high dose group and positive drug group increased significantly(P<0.05).The β-CTX level and RANKL protein level in serum were significantly reduced(P<0.05).Conclusion:Panlongqi tablet may inhibit the level of RANKL and up-regulate the expression of OPG to improve the osteoporosis of ovariectomized rats.

参考文献/References:

[1] 宋敏,周灵通,刘小钰,等.消化系统疾病并发骨质疏松症与肠道菌群的关系探讨[J].中国骨质疏松杂志,2018,24(1):130-134.
[2] 李永贤,张顺聪,梁德,等.淫羊藿苷对老龄大鼠去势后骨质疏松骨折的治疗作用及机制研究[J].中华中医药杂志,2018,33(8):3574-3579.
[3] 谢兴文,李建国,李宁,等.基于“益肾生髓”理论探讨绝经后骨质疏松症的病机及临床治疗[J].中国中医基础医学杂志,2019,25(11):1635-1638.
[4] 沈家峰,卢建华,徐孝平,等.红曲对去卵巢大鼠骨组织RANKL、OPG蛋白和mRNA表达的影响[J].中华中医药杂志,2018,33(4):1370-1372.
[5] LIU C,CHEN X,ZHI X,et al.Structure-based development of an osteoprotegerin-like glycopeptide that blocks RANKL/RANK interactions and reduces ovariectomy-induced bone loss in mice[J].Eur J Med Chem,2018,145(1):661-672.
[6] 巨振兴,徐永军.盘龙七片联合塞来昔布治疗类风湿性关节炎的临床研究[J].现代药物与临床,2018,33(11):3011-3015.
[7] 孟祥东,冯宇,李福龙,等.盘龙七片对膝骨关节炎大鼠软骨细胞凋亡的抑制及其作用机理[J].中国中医骨伤科杂志,2020,28(2):1-4.
[8] 李宁,姚新苗,龚怡辰.基于PI3K/AKT/GSK-3β信号通路探讨益骨汤对去势大鼠骨质疏松的作用[J].中国中医药科技,2021,28(5):708-711.
[9] 贾可欣,刘春芳,王金霞,等.盘龙七片对慢性炎性疼痛大鼠的镇痛作用[J].中国实验方剂学杂志,2020,26(15):61-68.
[10] 郜香敏.艾瑞昔布片联合盘龙七片在骨质疏松性椎体压缩骨折患者经皮椎体成形术后的应用效果[J].临床医学,2021,41(7):121-123.
[11] 颜春鲁,王琳,安方玉,等.地黄饮子水煎剂对去势骨质疏松大鼠生物力学及OPG/RANKL/RANK含量的影响[J].中华中医药杂志,2018,33(10):4642-4645.
[12] SI Z,ZHOU S,SHEN Z,et al.High-throughput metabolomics discovers metabolic biomarkers and pathways to evaluating the efficacy and exploring potential mechanisms of osthole against osteoporosis based on UPLC/Q-TOF-MS coupled with multivariate data analysis[J].Front Pharmacol,2020,11(1):741.
[13] PARDINI D.Hormone replacement therapy in menopause[J].Arq BrasEndocrinol Metabol,2014,58(2):172-181.
[14] 谢平金,史桐雨,梁桂洪,等.川芎嗪对膝骨性关节炎大鼠软骨BMP-2、Smad1及BMP-2 mRNA、Smad1 mRNA表达的影响[J].中国骨质疏松杂志,2018,24(6):727-731.
[15] 张晓强,李孟芳,刘品莉.盘龙七片联合同经相应取穴针刺法治疗膝骨关节炎的临床观察[J].中国民间疗法,2020,28(18):62-63.
[16] XIE X,LIU M,MENG Q.Angelica polysaccharide promotes proliferation and osteoblast differentiation of mesenchymal stem cells by regulation of long non-coding RNA H19:an animal study[J].Bone Joint Res,2019,8(7):323-332.
[17] QI S,ZHENG H,CHEN C,et al.Du-Zhong(Eucommia ulmoides Oliv.)cortex extract alleviates lead acetate-induced bone loss in rats[J].Biol Trace Elem Res,2019,187(1):172-180.
[18] ZHAO X,WANG Y,NIE Z,et al.Eucommia ulmoides leaf extract alters gut microbiota composition,enhances short-chain fatty acids production,and ameliorates osteoporosis in the senescence-accelerated mouse P6(SAMP6)model[J].Food Sci Nutr,2020,8(9):4897-4906.
[19] LIU L,TAO W,PAN W,et al.Hydroxysafflor yellow A promoted bone mineralization and inhibited bone resorption which reversed glucocorticoids-induced osteoporosis[J].Biomed Res Int,2018,2018(1):6762146.
[20] SHAO J,ZHANG Y,YANG T,et al.HIF-1α disturbs osteoblasts and osteoclasts coupling in bone remodeling by up-regulating OPG expression[J].In Vitro Cell Dev Biol Anim,2015,51(8):808-814.
[21] SZULC P,NAYLOR K,PICKERING M E,et al.Use of CTX-I and PINP as bone turnovermarkers:National Bone Health Alliance recommendations to standardize sample handling and patient preparation to reduce pre-analytical variability[J].Ann Biol Clin,2018,76(4):373-391.
[22] TIAN K,SU Y,DING J,et al.Hederagenin protects mice against ovariectomy-induced bone loss by inhibiting RANKL-induced osteoclastogenesis and bone resorption[J].Life Sci,2020,244(1):117336.
[23] 吴彬,张勇,陈明亮,等.雌马酚对去卵巢后大鼠骨质疏松症的影响[J].第三军医大学学报,2015,37(3):256-260.

备注/Memo

备注/Memo:
基金项目:盘龙七片上市再评价研究(2020ZDLSF03-10)
△通信作者 E-mail:lishuoqi2020@163.com
更新日期/Last Update: 2022-01-18