[1]于云祥 龚泰芳 刘小涛 柯文 李彬彬 廉凯 徐进△.过表达性别决定区Y框蛋白7基因对骨肉瘤细胞生长及免疫逃逸相关因子表达的影响[J].中国中医骨伤科杂志,2021,29(11):13-17.
　YU Yunxiang GONG Taifang LIU Xiaotao KE Wen LI Binbin LIAN Kai XU Jin.Mechanism of Overexpression of Sex Determining Region Y-box 7 Gene on the Growth of Osteosarcoma Cells and the Expression of Immune Escape Related Factors[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2021,29(11):13-17.
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过表达性别决定区Y框蛋白7基因对骨肉瘤细胞生长及免疫逃逸相关因子表达的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第29卷
期数:
2021年11期
页码:
13-17
栏目:
实验研究
出版日期:
2021-11-15

文章信息/Info

Title:
Mechanism of Overexpression of Sex Determining Region Y-box 7 Gene on the Growth of Osteosarcoma Cells and the Expression of Immune Escape Related Factors
文章编号:
1005-0205(2021)11-0013-05
作者:
于云祥1 龚泰芳1 刘小涛1 柯文1 李彬彬1 廉凯2 徐进2△
Author(s):
YU Yunxiang1 GONG Taifang1 LIU Xiaotao1 KE Wen1 LI Binbin1 LIAN Kai2 XU Jin2△
1The First Department of Orthopedics, Taihe Hospital, Shiyan 442000, Hubei China; 2Department of Orthopedics,Xiangyang Central Hospital Affiliated to Hubei University of Arts and Sciences,Xiangyang 441021,Hubei China.
关键词:
骨肉瘤;  性别决定区Y框蛋白7基因;  凋亡;  免疫逃逸
Keywords:
osteosarcoma;  sex determining region Y-box 7 gene;  apoptosis;  immune escape
分类号:
R738.1
文献标志码:
A
摘要:
目的:探讨过表达性别决定区Y框蛋白7(SOX7)基因对骨肉瘤MG-63细胞活力、周期、凋亡、Wnt/β-catenin信号及免疫逃逸相关VEGF和TGF-β1表达的影响。方法:将人骨肉瘤MG-63细胞分为空白组、pcDNA3.1组和pcDNA3.1-SOX7组,pcDNA3.1转染参照Lipofectamine 2000试剂盒说明书。通过CCK-8法检测pcDNA3.1-SOX7转染MG-63细胞24～72 h的细胞活力; 流式细胞仪检测pcDNA3.1-SOX7转染MG-63细胞48 h的细胞周期和凋亡率。Western Blot检测SOX7、β-catenin、cyclinD1、Survivin、VEGF和TGF-β1蛋白表达。结果:pcDNA3.1-SOX7转染MG-63细胞后,细胞中SOX7蛋白表达明显高于空白组,差异有统计学意义(P<0.05)。与空白组比较,pcDNA3.1-SOX7组细胞活力明显降低,凋亡率明显升高,G0/G1期细胞百分比明显升高,G2/M期和S期百分比明显降低,β-catenin、CyclinD1、Survivin、VEGF和TGF-β1蛋白表达明显降低,差异有统计学意义(P<0.05)。结论:过表达SOX7基因可抑制骨肉瘤MG-63细胞活力,阻碍细胞周期进程,诱导细胞凋亡,抑制免疫逃逸相关VEGF和TGF-β1表达,其机制与抑制Wnt/β-catenin信号通路有关。
Abstract:
Objective:To investigate the effect of SOX7 overexpression on the viability, cycle, apoptosis, Wnt/β-catenin signal and the expression of immune escape-related VEGF and TGF-β1 in osteosarcoma MG-63 cells.Methods:Human osteosarcoma MG-63 cells were divided into blank group, pcDNA3.1 group and pcDNA3.1-SOX7 group.The pcDNA3.1 was transfected according to Lipofectamine 2000 kit.The viability of MG-63 cells transfected with pcDNA3.1-SOX7 for 24 to 72 h was detected by CCK-8 method, and the cell cycle and apoptosis rate of MG-63 cells transfected with pcDNA3.1-SOX7 for 48 h were detected by flow cytometry.Western Blot was used to detect the expression of SOX7, β-catenin, cyclinD1, survivin, VEGF and TGF-β1 protein.Results:The expression of SOX7 protein in MG-63 cells transfected with pcDNA3.1-SOX7 was significantly higher than that in blank group(P<0.05).Compared with the blank group, the cell viability of pcDNA3.1-SOX7 group decreased significantly, the apoptosis rate increased significantly, the percentage of G0/G1 phase cells increased significantly, the percentage of G2/M phase and S phase cells decreased significantly, and the expression of β-catenin, cyclinD1, survivin, VEGF and TGF-β1 protein decreased significantly(P<0.05).Conclusion:Overexpression of SOX7 gene can inhibit the viability of osteosarcoma MG-63 cells, block cell cycle progression, induce apoptosis, and inhibit the expression of immune escape-related VEGF and TGF-β1.The mechanism is related to the inhibition of Wnt/β-catenin signaling pathway.

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备注/Memo

备注/Memo:
基金项目:湖北省卫生计生委科研项目(JX6B36)
1湖北十堰市太和医院骨一科(湖北 十堰,442000)
2湖北文理学院附属医院(襄阳市中心医院)骨科
△通信作者 E-mail:516289565@qq.com
更新日期/Last Update: 1900-01-01