[1]吴波文 蔡伟良 刘欢欢 刘银 李洁 朱宝玉 曾伟△.假体周围感染兔模型中D-二聚体的动态表达与感染时间和种植细菌量的相关性研究[J].中国中医骨伤科杂志,2021,29(11):7-12.
　WU Bowen CAI Weiliang LIU Huanhuan LIU Yin LI JieZHU Baoyu ZENG Wei.Study on the Correlation between the Dynamic Expression of D-Dimer and the Infection Time and the Amount of Bacteria Planted in the Rabbit Model of Prosthesis Infection[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2021,29(11):7-12.
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假体周围感染兔模型中D-二聚体的动态表达与感染时间和种植细菌量的相关性研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第29卷
期数:
2021年11期
页码:
7-12
栏目:
实验研究
出版日期:
2021-11-15

文章信息/Info

Title:
Study on the Correlation between the Dynamic Expression of D-Dimer and the Infection Time and the Amount of Bacteria Planted in the Rabbit Model of Prosthesis Infection
文章编号:
1005-0205(2021)11-0007-06
作者:
吴波文1 蔡伟良2 刘欢欢3 刘银3 李洁3 朱宝玉3 曾伟3△
Author(s):
WU Bowen1 CAI Weiliang2 LIU Huanhuan3 LIU Yin3 LI Jie3ZHU Baoyu3 ZENG Wei3△
1Department of Traumatology and Orthopedics, Zhuzhou Central Hospital of Hunan Province, Zhuzhou 412000,Hunan China; 2Department of Orthopedics, The Second Xiangya Hospital of Central South University, Changsha 410011,China; 3Department of Joint Surgery, Zhuzhou Central Hospital of Hunan Province, Zhuzhou 412000,Hunan China.
关键词:
假体周围感染兔模型;  D-二聚体;  金黄色葡萄球菌
Keywords:
rabbit model of infection around prosthesis;  D-dimer;  staphylococcus aureus
分类号:
R-33
文献标志码:
A
摘要:
目的:研究D-二聚体的表达水平在假体周围感染(Prosthetic Joint Infections,PJI)兔模型中与细菌感染时间和种植菌量的相关性。方法:用金黄色葡萄球菌(Methicillin-Resistant Staphylococcus Aureus,MSSA)建立PJI大白兔模型,分别以0 cfu/mL(仅注射生理盐水,MSSA-A组)、0.5×104 cfu/mL(MSSA-B组)、0.5×105 cfu/mL(MSSA-C组)、0.5×106 cfu/mL(MSSA-D组)的MSSA进行感染,每组各10只。扫描电镜(Scanning Electron Microscope,SEM)观察细菌生物膜形成。试剂盒法检测PJI术前及术后1,7,13 d各组血液中的C反应蛋白(C-Reactive Protein,CRP)、血沉(Erythrocyte Sedimentation Rate,ESR)及D-二聚体的浓度变化。分析D-二聚体的动态表达与感染时间和细菌浓度之间的相关性。结果:术后13 d各组关节腔液标本中的细菌类型与接种菌一致,均为MSSA。与MSSA-A组比,术后1,7,13 d的MSSA-B组、MSSA-C组、MSSA-D组中的C反应蛋白(F=215.369,189.115,155.698; P=1.153×10-6,2.559×10-5,3.662×10-6)、血沉(F=170.691,206.445,181.229; P=8.169×10-5,6.182×10-6,5.177×10-5)、D-二聚体(F=155.600,189.662,89.710; P=1.821×10-4,5.299×10-5,4.058×10-4)均持续增加,13 d的假体周围细菌阳性率也增加,差异均有统计学意义(F=214.366,P=6.125×10-5)。另外,术后1,7,13 d D-二聚体的表达水平与细菌浓度都正相关(P=0.003,0.002,0.001; r=0.921,0.955,0.923)。结论:PJI兔模型中13 d内D-二聚体的表达随时间增加而增加,D-二聚体的动态表达与种植细菌量正相关。
Abstract:
Objective:To study the correlation between the dynamic expression of D-dimer and the infection time and the amount of bacteria planted in the rabbit model of prosthesis joint infection.Methods:Staphylococcus aureus(MSSA)was used to establish a PJI white rabbit model.Rabbits were infected with 0 cfu/mL(injection of saline only, MSSA-A group), 0.5×104 cfu/mL(MSSA-B group), 0.5×105 cfu/mL(MSSA-C group), 0.5×106 cfu/mL(MSSA-D group)respectively, n=10.Scanning electron microscope(SEM)was used to observe the formation of bacterial biofilm.The kit method was used to detect the concentration changes of C-reactive protein(CRP), erythrocyte sedimentation rate(ESR)and D-dimer in the blood of each group before PJI and 1 d, 7 d and 13 d after PJI operation.The correlation between the dynamic expression of D-dimer and the infection time and bacterial concentration was analyzed.Results:13 d after operation, the types of bacteria in the joint cavity fluid samples of each group were consistent with the inoculated bacteria, and they were all MSSA.Compared with MSSA-A group, the CRP of MSSA-B group, MSSA-C group and MSSA-D group at 1, 7 and 13 d after surgery(F=215.369, 189.115, 155.698; P=1.153×10-6, 2.559×10-5, 3.662×10-6),ESR(F=170.691, 206.445, 181.229; P=8.169×10-5, 6.182×10-6, 5.177×10-5), D-dimer(F=155.600, 189.662, 89.710; P=1.821×10-4, 5.299×10-5, 4.058×10-4), and the positive rate of bacteria around the prosthesis at 13 d also were increased, and the differences were statistically significant(F=214.366,P=6.125×10-5).In addition, the expression level of D-dimer was positively correlated with the bacterial concentration at 1 d, 7 d and 13 d postoperatively(P=0.003, 0.002, 0.001; r=0.921, 0.955, 0.923).Conclusion:The dynamic expression of D-dimer in the PJI rabbit model is positively correlated with the infection time and the number of bacteria grown.

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备注/Memo

备注/Memo:
基金项目:湖南创新型省份建设专项任务(2019JJ60086)
1湖南株洲市中心医院创伤骨科(湖南 株洲,412000)
2中南大学湘雅二医院骨科
3湖南株洲市中心医院关节外科
△通信作者 E-mail:aabu123@163.com
更新日期/Last Update: 1900-01-01