[1]马晓程 赵亚君 毛世刚△.祛寒逐风颗粒对骨性关节炎大鼠炎症反应的影响[J].中国中医骨伤科杂志,2021,29(09):16-21.
　MA Xiaocheng ZHAO Yajun MAO Shigang.Efficacy of Quhan Zhufeng Granule on InflammatoryResponse in Rats with Osteoarthritis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2021,29(09):16-21.
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祛寒逐风颗粒对骨性关节炎大鼠炎症反应的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第29卷
期数:
2021年09期
页码:
16-21
栏目:
实验研究
出版日期:
2021-09-15

文章信息/Info

Title:
Efficacy of Quhan Zhufeng Granule on InflammatoryResponse in Rats with Osteoarthritis
文章编号:
1005-0205(2021)09-0016-06
作者:
马晓程1 赵亚君2 毛世刚2△
1青岛市市立医院骨科(山东 青岛,266011)
2青岛市市立医院康复医学科
Author(s):
MA Xiaocheng1 ZHAO Yajun2 MAO Shigang2△
1Department of Orthopaedics,Qingdao Municipal Hospital,Qingdao 266011,Shandong China; 2Department of Rehabilitation Medicine,Qingdao Municipal Hospital,Qingdao 266011,Shandong China.
关键词:
祛寒逐风颗粒;  骨性关节炎;  炎性小体
Keywords:
Quhan Zhufeng granules;  osteoarthritis;  inflammasome
分类号:
R-33
文献标志码:
A
摘要:
目的:观察祛寒逐风颗粒对膝骨性关节炎(KOA)大鼠炎症反应和NF-κB介导NLRP3炎性小体活化的影响。方法:建立骨性关节炎大鼠模型,随机分为假手术组、模型组、祛寒逐风颗粒组(3,6,9 g/kg)和阳性药组,测量大鼠机械性痛阈值和膝关节直径,采用酶联免疫吸附实验测量大鼠血清中白细胞介素(IL)-1β、肿瘤坏死因子(TNF)-α和IL-6的含量变化,采用RT-PCR法测量大鼠组织中IL-1β、TNF-α和IL-6的mRNA含量,采用苏木精-伊红染色和阿尔新蓝染色观察组织的病理学变化,采用蛋白免疫印迹法检测大鼠软骨组织中p-p65、p65、p-IκBα、NLRP3、Caspase-1 p20、IL-1β p17的蛋白水平。结果:与模型组比较,祛寒逐风颗粒组和阳性药组大鼠的机械性痛阈值增大,膝关节直径变小,血清中IL-1β、TNF-α和IL-6含量减少,组织中IL-1β、TNF-α和IL-6 mRNA水平下调,软骨损伤和纤维化缓解,软骨组织中p-p65、p65、p-IκBα、NLRP3、Caspase-1 p20、IL-1β p17蛋白表达下调。结论:祛寒逐风颗粒可能通过抑制NF-κB介导的NLRP3炎性小体活化显着改善KOA大鼠的炎症反应并缓解疼痛,对骨关节炎发挥治疗作用。
Abstract:
Objective:To observe the efficacy of Quhan Zhufeng granules on inflammatory response and NF-κB-mediated activation of NLRP3 inflammasomes in rats with knee osteoarthritis.Methods:Rat models with osteoarthritis were established and randomly divided into sham operation group,model group,Quhan Zhufeng granule(3,6,9 g/kg)group and positive drug group.Mouse mechanical pain threshold and knee joint diameter was analyzed.The enzyme-linked immunosorbent assay was used to measure the level changes of interleukin(IL)-1β,tumor necrosis factor(TNF)-α and IL-6 in rat serum.The RT-PCR was used to measure the levels of IL-1β,TNF-α and IL-6 mRNA in rat tissues.Hematoxylin-eosin staining and Alcian blue staining were used to observe the pathological changes of the tissues.The protein levels of p-p65,p65,p-IκBα,NLRP3,Caspase-1 p20,and IL-1β p17 in rat cartilage tissues were detected by Western Blot.Results:Compared with the model group,the mechanical pain threshold was increased,the diameter of the knee joint was decreased in the Quhan Zhufeng granule group and the positive drug group,the contents of IL-1β,TNF-α and IL-6 in serum were decreased,the levels of IL-1β,TNF-α and IL-6 mRNA in tissues were down-regulated,the cartilage damage and fibrosis were relieved,and the protein expression of p-p65,p65,p-IκBα,NLRP3,Caspase-1 p20,IL-1β p17 in cartilage tissue of the rats in the Quhan Zhufeng granule group and the positive drug group was down-regulated.Conclusion:Quhan Zhufeng granules may significantly improve the inflammatory response and relieve pain of KOA rats by inhibiting the activation of NLRP3 inflammatory bodies mediated by NF-κB,and play a therapeutic efficacy on osteoarthritis.

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备注/Memo

备注/Memo:
基金项目:吴阶平医学基金会临床科研专项资助基金(320.6710.17216)
△通信作者 E-mail:eavorforever@163.com
更新日期/Last Update: 1900-01-01