[1]展嘉文 朱立国 王尚全△ 魏戌 刘广伟 王晓博王文轩 谢瑞 韩涛 陈明 尹煜辉.丹酚酸A治疗在体腰椎小关节骨性关节炎的实验研究[J].中国中医骨伤科杂志,2021,29(07):7-11.
　ZHAN Jiawen ZHU Liguo WANG Shangquan WEI Xu LIU GuangweiWANG Xiaobo WANG Wenxuan XIE Rui HAN Tao CHEN Ming YIN Yuhui.Efficacy of Salvianolic Acid A on Lumbar FacetJoint Osteoarthritis in Rabbits in Vivo[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2021,29(07):7-11.
点击复制

丹酚酸A治疗在体腰椎小关节骨性关节炎的实验研究()
分享到：

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第29卷
期数:
2021年07期
页码:
7-11
栏目:
实验研究
出版日期:
2021-07-15

文章信息/Info

Title:
Efficacy of Salvianolic Acid A on Lumbar FacetJoint Osteoarthritis in Rabbits in Vivo
文章编号:
1005-0205(2021)07-0007-05
作者:
展嘉文1; 2 朱立国1; 2 王尚全1; 2△ 魏戌1; 2 刘广伟1; 2 王晓博1王文轩3 谢瑞1 韩涛1 陈明1 尹煜辉1
1中国中医科学院望京医院(北京,100102)2中医正骨技术北京市重点实验室3陕西中医药大学
Author(s):
ZHAN Jiawen1; 2 ZHU Liguo1; 2 WANG Shangquan1; 2△ WEI Xu1; 2 LIU Guangwei1; 2WANG Xiaobo1 WANG Wenxuan3 XIE Rui1 HAN Tao1 CHEN Ming1 YIN Yuhui1
1Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102, China; 2Key Laboratory of Beijing of Chinese Medical Orthopaedic Technology, Beijing 100102, China; 3Shaanxi University of Traditional Chinese Medicine, Xianyang 712046,Shaanxi China.
关键词:
腰椎小关节;  骨关节炎;  应力退变;  丹酚酸A
Keywords:
lumbar facet joints;  osteoarthritis;  compression degeneration;  salvianolic acid A
分类号:
R-33
文献标志码:
A
摘要:
目的:观察丹酚酸A对持续压力导致的腰椎小关节骨性关节炎的干预作用。方法:采用在体加压装置,建立在体兔脊柱运动节段压力退变模型。将24只新西兰白兔随机分为对照组、模型组与丹酚酸组,其中模型组在L4～5节段予以持续294 kPa压力,丹酚酸组在持续压力基础上予腹腔注射丹酚酸A干预。术后第28天各组切取双侧L4～5小关节,分离关节软骨进行番红固绿染色,采用流式细胞术测定软骨细胞凋亡情况,分离滑膜组织采用qPCR与Western blot法检测滑膜组织炎症因子表达情况。结果:模型组腰椎小关节软骨表面形态不平整,出现裂隙,丹酚酸组软骨表面相对光滑,但染色欠均匀。模型组软骨细胞显著凋亡,而丹酚酸组则下调了该模型中的凋亡比例,差异有统计学意义(P<0.05)。相比模型组,丹酚酸干预后的腰椎小关节滑膜组织中炎症因子IL-1β、TNF-α、IL-6的mRNA与蛋白表达水平出现显著降低,差异有统计学意义(P<0.05)。结论:丹酚酸A能够缓解持续压力对腰椎小关节软骨造成的损伤程度,抑制软骨细胞凋亡与滑膜组织中炎症因子的表达。
Abstract:
To observe the efficacy of salvianolic acid A on lumbar facet joint osteoarthritis caused by constant compression.Methods: In-vivo compression device was used to establish the constant compression model of rabbit spinal motion segment.24 New Zealand white rabbits were randomly divided into controls, the load group, and the salvianolic acid group.The load group was given constant compression of 294 kPa at the lumbar 4/5 segment, and the salvianolic acid group was given an intraperitoneal injection of salvianolic acid A based on constant compression.On the 28th day after the operation, bilateral L4/L5 facets were harvested from each group.The articular cartilage was separated and stained with safranin and solid green.The apoptosis of chondrocytes was detected by flow cytometry.The expression of inflammatory factors of IL-1β、TNF-α、IL-6 in synovial tissue was detected by qPCR and Western Blot.Results: The surface of the articular cartilage of lumbar facet joints was uneven with cracks in the load group.The surface of cartilage was relatively smooth in the salvianolic acid group, but the staining was not uniform.Chondrocytes in the load group were significantly apoptotic, while the salvianolic acid group decreased the proportion of apoptosis in the model(P<0.05).Compared with the model group, the mRNA and Western Blot expression levels of inflammatory factors IL-1 β, TNF-α, and IL-6 in lumbar facet joint synovial tissue were significantly decreased(P<0.05)after the salvianolic acid intervention.Conclusion: Salvianolic acid A can alleviate the damage of lumbar facet articular cartilage caused by constant compression, inhibit chondrocyte apoptosis, and the expression of inflammatory factors in synovial tissue.

参考文献/References:

[1] BERESFORD Z M,KENDALL R W,WILLICK S E.Lumbar facet syndromes[J].Current Sports Medicine Reports,2010,9(1):50-56.
[2] MOBASHERI A.Intersection of inflammation and herbal medicine in the treatment of osteoarthritis[J].Curr Rheumatol Rep,2010,14(6):604-616.
[3] CHOU R,HUFFMAN L H,SOCIETY A P,et al.Nonpharmacologic therapies for acute and chronic low back pain:a review of the evidence for an american pain society/American college of physicians clinical practice guideline[J].Annals of Internal Medicine,2007,147(7):492-504.
[4] AITKEN D,JONES G,WINZENBERG T.Clinical overview of osteoarthritis(OA)and the challenges faced for future management[C]//Encyclopedia of Biomedical Gerontology.Amsterdam:Elsevier,2020:420-430.
[5] 展嘉文,朱立国,冯敏山,等.丹参注射液含药血清对离体培养兔脊柱运动节段椎间盘的影响[J].中华中医药杂志,2015,30(5):1834-1837.
[6] WU Y,WANG Z,LIN Z,et al.Salvianolic acid A has anti-osteoarthritis effect in vitro and in vivo[J].Frontiers in Pharmacology,2020,11:682.
[7] KROEBER M W,UNGLAUB F,WANG H,et al.New in vivo animal model to create intervertebral disc degeneration and to investigate the effects of therapeutic strategies to stimulate disc regeneration[J].Spine(Phlia Pa 1976),2002,27(23):2684.
[8] KALICHMAN L,HUNTER D J.Lumbar facet joint osteoarthritis:a review[J].Seminars in Arthritis & Rheumatism,2008,37(2):69-80.
[9] ADAMS M A,HUTTON W C.The mechanical function of the lumbar apophyseal joints[J].Spine,1983,8(3):327-330.
[10] PARK W M,KIM K,KIM Y H.Effects of degenerated intervertebral discs on intersegmental rotations,intradiscal pressures,and facet joint forces of the whole lumbar spine[J].Computers in Biology & Medicine,2013,43(9):1234-1240.
[11] NAKAMURA A,RAMPERSAUD Y R,SHARMA A,et al.Identification of microRNA-181a-5p and microRNA-4454 as mediators of facet cartilage degeneration[J].JCI Insight,2016,1(12):e86820.
[12] 殷刚,邱勇.腰椎小关节骨性关节炎危险因素的研究进展[J].中国矫形外科杂志,2019,17(13):995-997.
[13] XU D,SUN Y,BAO G,et al.MMP-1 overexpression induced by IL-1β:possible mechanism for inflammation in degenerative lumbar facet joint[J].J Orthop Sci,2013,18(6):1012-1019.
[14] YEH T T,WEH Z H,LEE H S,et al.Intra-articular injection of collagenase induced experimental osteoarthritis of the lumbar facet joint in rats[J].European Spine Journal,2008,17(5):734-742.
[15] GONG K,SHAO W,CHEN H,et al.Rat model of lumbar facet joint osteoarthritis associated with facet-mediated mechanical hyperalgesia induced by intra-articular injection of monosodium iodoacetate[J].Journal of the Formosan Medical Association,2011,110(3):145-152.
[16] SHUANG F,ZHU J,SONG K,et al.Establishment of a rat model of adjuvant-induced osteoarthritis of the lumbar facet joint[J].Cell Biochemistry & Biophysics,2014,70(3):1545-1551.
[17] SHUANG F,HOU S X,ZHU J L,et al.Establishment of a rat model of lumbar facet joint osteoarthritis using intraarticular injection of urinary plasminogen activator[J].Scientific Reports,2015,5:9828.
[18] 张继业,王吉兴,张斌,等.高应力导致兔腰椎小关节骨性关节炎的实验研究[J].中国脊柱脊髓杂志,2011,21(10):853-859.
[19] 朱立国,展嘉文,冯敏山,等.补肾活血方治疗椎间盘源性腰痛的临床观察[J].世界中医药,2017,12(3):554-557.
[20] 韩涛,朱立国,展嘉文,等.补肾活血中药治疗下腰痛疗效Meta分析[J].辽宁中医药大学学报,2019,21(4):127-130.
[21] 刘慧,开金龙.丹参的现代研究进展[J].甘肃中医,2010,23(2):70-72.
[22] 赵仁霞.丹参的现代药理研究及临床应用[J].中国医药指南,2011,9(12):291-292.
[23] 曹金仪.丹参的化学成分及临床用途[J].中国医药指南,2012,10(29):53-55.
[24] FAN H Y,FU F H,YANG M Y,et al.Antiplatelet and antithrombotic activities of salvianolic acid A[J].Thrombosis Research,2010,126(1):e17-e22.
[25] LIU C H,HU Y Y,WANG X L,et al.Effectsof salvianolic acid-A on NIH/3T3 fibroblast proliferation,collagen synthesis and gene expression[J].World Journal of Gastroenterology,2000,6(3):361-364.
[26] WANG C Y,MA F L,LIU J T,et al.Protective effect of salvianic acid A on acute liver injury induced by carbon tetrachloride in rats[J].Biological & Pharmaceutical Bulletin,2007,30(1):44-47.
[27] WANG T,SHAN S Y,HAN B,et al.Salvianolic acid A exerts antiamnesic effect on diazepam-induced anterograde amnesia in mice[J].Neurochemical Research,2011,36(1):103-108.
[28] FENG S,CONG H,JI L.Salvianolic acid A exhibits anti-inflammatory and antiarthritic effects via inhibiting NF-κB and p38/MAPK pathways[J].Drug Design Development & Therapy,2020,14:1771-1778.
[29] WANG R,SONG F X,LI S N,et al.Salvianolic acid A attenuates CCl4-induced liver fibrosis by regulating the PI3K/AKT/mTOR,Bcl-2/Bax and caspase-3/cleaved caspase-3 signaling pathways[J].Drug Design Development & Therapy,2019,13:1889-1990.
[30] ZU G,ZHOU T,CHE N,et al.Salvianolic acid a protects against oxidative stress and apoptosis induced by intestinal ischemia-reperfusion injury through activation of Nrf2/HO-1 pathways[J].Cellular Physiology & Biochemistry,2018,49(6):2320-2332.
[31] 牛子冉,徐晓娜,陈俞材,等.丹酚酸A对异丙肾上腺素致小鼠心肌缺血的保护作用及其机制[J].中国药理学通报,2015,31(12):1667-1674.

备注/Memo

备注/Memo:
基金项目:中国中医科学院优秀青年科技人才培养专项(ZZ13-YQ-038)国家体育总局中医特色技术在体育运动中的应用(HXKT2017001)
△通信作者 E-mail:zhanjiawen12@126.com
更新日期/Last Update: 2021-07-15