[1]宋凯 潘乐 李智斌 王官林 孙晓洁 刘康 唐少雄 杨利学△.右归丸对肝肾亏虚型退变髓核细胞活力及相关炎性因子表达的影响[J].中国中医骨伤科杂志,2021,29(07):12-16.
　SONG Kai PAN Le LI Zhibin WANG Guanlin SUN XiaojieLIU Kang TANG Shaoxiong YANG Lixue.Efficacy of Yougui Pill on Cell Viability and Expression ofRelated Inflammatory Factors in Degenerative NucleusPulposus of Human with Deficiency of Liver and Kidney[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2021,29(07):12-16.
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右归丸对肝肾亏虚型退变髓核细胞活力及相关炎性因子表达的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第29卷
期数:
2021年07期
页码:
12-16
栏目:
实验研究
出版日期:
2021-07-15

文章信息/Info

Title:
Efficacy of Yougui Pill on Cell Viability and Expression ofRelated Inflammatory Factors in Degenerative NucleusPulposus of Human with Deficiency of Liver and Kidney
文章编号:
1005-0205(2021)07-0012-05
作者:
宋凯1 潘乐2 李智斌2 王官林2 孙晓洁2 刘康3 唐少雄1 杨利学1△
1陕西中医药大学(陕西 咸阳,712000)2陕西中医药大学附属医院3陕西中医药大学第二附属医院
Author(s):
SONG Kai1 PAN Le2 LI Zhibin2 WANG Guanlin2 SUN Xiaojie2LIU Kang3 TANG Shaoxiong1 YANG Lixue1△
1Shaanxi University of Traditional Chinese Medicine, Xianyang 712000, Shaanxi China; 2Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, Xianyang 712000, Shaanxi China; 3Second Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, Xianyang 712000, Shaanxi China.
关键词:
右归丸;  桃红四物汤;  髓核细胞;  炎症因子
Keywords:
Yougui pill;  Taohong Siwu decoction;  nucleus pulposus cells;  inflammatory factors
分类号:
R-33
文献标志码:
A
摘要:
目的:研究右归丸含药大鼠血清对肝肾亏虚型退变髓核细胞活性及IL-1、TNF-α表达的影响。方法:选取48只雌雄各半质量为180～220 g SD大鼠,按随机数字表法分为空白对照组、桃红四物汤组及右归丸组,各组药物干预后取血清冻存备用。选3例辨证肝肾亏虚型的腰椎间盘突出且需要手术的患者,获取髓核组织细胞并进行细胞培养。各组冻存的含药血清分别配置成5%、10%、20%浓度梯度干预髓核细胞,运用MTT法获取细胞OD值并制作细胞生长曲线,选取各组最佳含药血清浓度。将2代髓核细胞分为空白血清组、桃红血清组、右归血清组。选用各组最佳浓度血清分别连续干预3、7、14 d,Western Blot检测2代髓核细胞中IL-1、TNF-α的表达水平。结果:10%空白血清、20%桃红四物汤含药血清及20%右归丸含药血清有利于髓核细胞生长。分别在第3、7、14天与空白血清组比较,桃红血清组、右归血清组髓核细胞中IL-1、TNF-α的表达水平明显降低,差异有统计学意义(P<0.05),且右归血清组相比桃红血清组IL-1、TNF-α降低更加明显,差异有统计学意义(P<0.05)。结论:右归丸大鼠含药血清干预肝肾亏虚型退变髓核细胞可显著提高细胞增殖活力,下调炎性因子 IL-1、TNF-α的表达,右归丸含药血清干预肝肾亏虚型退变髓核细胞效果优于桃红四物汤含药血清,从侧面进一步论证了辨证论治理论的科学性。
Abstract:
To study the efficacy of Yougui pill-containing rat serum on the activity of degenerated nucleus pulposus cells and the expression of IL-1 and TNF-α in patients with liver and kidney deficiency type.Methods:48 male and female SD rats with a weight of 180 to 220 g each and divide them into blank control group, Taohong Siwu decoction group and Yougui pill group according to the random number table.Serum of each group is frozen and stored after drug intervention spare.3 cases of patients with a herniated lumbar intervertebral disc with liver and kidney deficiency and required surgery were selected to obtain nucleus pulposus tissue cells and carry out cell culture.The cryopreserved medicated serum of each group was configured into 5%, 10%, 20% concentration gradients to interfere with the nucleus pulposus cells.The cell OD value was obtained by the MTT.The cell growth curve was made, and the best drug-containing serum concentration of each group was selected.The 2 generations of nucleus pulposus cells were divided into a blank serum group, a Taohong serum group, and Yougui serum group.The optimal concentration of serum in each group was selected for continuous intervention for 3, 7 and 14 d respectively, and the expression levels of IL-1 and TNF-α in the second-generation nucleus pulposus cells were detected by Western Blot.Results: 10% blank serum, 20% Taohong Siwu decoction containing serum and 20% Yougui pill containing serum were all beneficial to the growth of nucleus pulposus cells.Compared with the blank serogroup at 3rd, 7th and 14th day, the expression levels of IL-1 and TNF-α in the nucleus pulposus cells of the Taohong serogroup and Yougui serogroup in these times were significantly reduced, and the difference was statistically significant(P<0.05).The levels of IL-1 and TNF-α in the Yougui serogroup were significantly lower than those in the taohong serum group.Conclusion: The intervention o of Yougui pill-containing rat serum on degenerated nucleus pulposus cells with liver and deficiency can significantly increase cell proliferation and down-regulate the expression of inflammatory factors IL-1 and TNF-α.The efficacy of degenerative nucleus pulposus cells of kidney deficiency type intervened by Yougui pill-containing serum is better than that of Taohong Siwu decoction containing medicated serum, which further demonstrates the scientific nature of the theory of syndrome differentiation and treatment from the side.

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备注/Memo

备注/Memo:
基金项目:陕西省自然科学基础研究计划项目(S2018-JC-YB-1870)
△通信作者 E-mail:yanglixue@sina.com
更新日期/Last Update: 2021-07-15