[1]樊燕鑫 夏天卫 沈计荣△.温肾宣痹方对大鼠膝骨关节炎软骨的保护作用研究[J].中国中医骨伤科杂志,2021,29(06):5-8.
　FAN Yanxin XIA Tianwei SHEN Jirong.The Study of Protective Efficacy of Wenshenxuanbi Decoction onChondrocyte of Knee Osteoarthritis in Rats[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2021,29(06):5-8.
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温肾宣痹方对大鼠膝骨关节炎软骨的保护作用研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第29卷
期数:
2021年06期
页码:
5-8
栏目:
实验研究
出版日期:
2021-06-15

文章信息/Info

Title:
The Study of Protective Efficacy of Wenshenxuanbi Decoction onChondrocyte of Knee Osteoarthritis in Rats
文章编号:
1005-0205(2021)06-0005-04
作者:
樊燕鑫1 夏天卫1 沈计荣1△
1南京中医药大学附属医院/江苏省中医院(南京,210029)
Author(s):
FAN Yanxin1 XIA Tianwei1 SHEN Jirong1△
1Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029,China.
关键词:
温肾宣痹方;  骨性关节炎;  炎症;  评分
Keywords:
wenshenxuanbi decoction;  osteoarthritis;  inflammation;  score
分类号:
R-33
文献标志码:
A
摘要:
目的:探究温肾宣痹方对大鼠膝骨关节炎(Osteoarthritis,OA)的作用效果及机制。方法:建立前交叉韧带切断的大鼠骨性关节炎动物模型,实验组给予温肾宣痹方灌胃8周,对照组、空白组给予纯净水灌胃8周。观察各组大鼠的国际骨关节炎研究协会(Osteoarthritis Research Society International,OARSI)评分以及软骨组织中MMP-1、MMP-13的基因的相对表达、滑膜组织中miR-146a的相对表达。结果:温肾宣痹方组的OARSI评分较对照组低、软骨组织中MMP-1、MMP-13的相对表达量较对照组低、滑膜组织中miR-146a的相对表达量较对照组高,差异有统计学意义(P<0.05)。结论:灌胃温肾宣痹方水煎剂对大鼠骨性关节炎具有一定的软骨保护效果,可延缓OA进程,机制与增加滑膜组织中miR-146a的表达相关。
Abstract:
To investigate the efficacy and potential mechanism of Wenshenxuanbi decoction(WSXBD)on knee osteoarthritis(OA)in rats. Methods:Rats model of anterior cruciate ligament(ACL)transection were established. The experimental group was given WSXBD for 8 weeks, while the control group and the blank group was given pure water for 8 weeks. The Osteoarthritis Research Society International(OARSI)score, the relative expression of MMP-1, MMP-13 in cartilage and miR-146a in synovium were observed.Results:The OARSI score of WSXBD was lower than that in control group, the relative expression of MMP-1 and MMP-13 was lower than that in control group, and the relative expression of miR-146a was higher than that in control Group. Conclusion:WSXBD has a protective efficacy on the cartilage of rat osteoarthritis, which can delay the progress of OA. The mechanism is related to the increase of miR-146a expression.

参考文献/References:


[1] 袁普卫,康武林,李小群,等.骨性关节炎发病机制及相关细胞因子的研究进展[J].中国矫形外科杂志,2016,24(11):1010-1015.
[2] 刘志队,史丽璞,桂银莉,等.补脾温肾宣痹汤对强直性脊柱炎(AS)患者疗效及生活质量的影响[J].数理医药学杂志,2017,30(8):1215-1216.
[3] 曾秋萍,戴琴香.温肾宣痹汤与物理因子治疗骨质增生腰痛51例小结[J].亚太传统医药,2008,4(7):55-56.
[4] 魏成建.诸方受运用温肾宣痹汤经验举隅[J].辽宁中医杂志,2006,33(6):744-745.
[5] ZHOU G W,LI R Q,XIA T W,et al.Utilizing network pharmacology to explore the underlying mechanism of cinnamomum cassia presl in treating osteoarthritis[J].International Journal of Clinical and Experimental Medicine,2019,12(12):13359-13369.
[6] XIA T W,HUAN D W,MAO G Q,et al.Utilizing network pharmacology to explore the underlying mechanism of cinnamomum cassia presl in treating osteoarthritis[J].International Journal of Clinical and Experimental Medicine,2020,13(10):7427-7436.
[7] XIA T W,GAO R Z,ZHOU G W,et al.Trans-cinnamaldehyde inhibits IL-1 β-stimulated inflammation in chondrocytes by suppressing NF-κB and p38-JNK pathways and exerts chondrocyte protective effects in a rat model of osteoarthritis[J].BioMed Research International,2019:4039472.
[8] 黄聪琳,李晓东,谢兴文,等.膝骨关节炎生物标记物研究进展[J].中国中医骨伤科杂志,2019,27(4):81-84.
[9] 周圆家,邹磊,董金波,等.miR-146a/27a在SD大鼠OA软骨、滑膜组织中的表达及意义[J].安徽医科大学学报,2015,50(3):275-279.
[10] 王欢,唐学章,丁海涛,等.桂皮醛对miRNA-146a干扰的骨关节炎滑膜炎性反应影响的实验研究[J].世界中医药,2017,12(10):2408-2413.
[11] FUNAHASHI Y.MiR-146a targeted to splenic macrophages prevents sepsis-induced multiple organ injury[J].Lab Invest,2019,99(8):1130-1142.
[12] WANG J.MiR-146a mimic attenuates murine allergic rhinitis by downregulating TLR4/TRAF6/NF-κB pathway[J].Immunotherapy,2019,11(13):1095-1105.
[13] ZHANG J.MiR-146a promotes oligodendrocyte progenitor cell differentiation and enhances remyelination in a model of experimental autoimmune encephalomyelitis[J].Neurobiology of Disease,2019,125:154-162.
[14] LI D,DUAN M Y,FENG Y,et al.MiR-146a modulates macrophage polarization in systemic juvenile idiopathic arthritis by targeting INHBA[J].Mol Immunol,2016,77:205-212.

备注/Memo

备注/Memo:
基金项目:江苏省中医院2018年院级创新发展基金专项课题(Y2018CX54)
△通信作者 E-mail:joint66118@sina.com
更新日期/Last Update: 2021-06-15