[1]陈谱 阮安民 周俊 张晓哲 宗晨钟 王庆甫△.通络止痛凝胶治疗膝骨关节炎的实验研究[J].中国中医骨伤科杂志,2020,28(09):1-5.
　CHEN Pu RUAN Anmin ZHOU Jun ZHANG Xiaozhe ZONG Chenzhong WANG Qingpu.Research Studyof Tongluo Zhitong Gel for Synovial Inflammation of Rabbit Knee Osteoarthritis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2020,28(09):1-5.
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通络止痛凝胶治疗膝骨关节炎的实验研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第28卷
期数:
2020年09期
页码:
1-5
栏目:
实验研究
出版日期:
2020-09-10

文章信息/Info

Title:
Research Studyof Tongluo Zhitong Gel for Synovial Inflammation of Rabbit Knee Osteoarthritis
文章编号:
1005-0205(2020)09-0001-05
作者:
陈谱1 阮安民1 周俊1 张晓哲1 宗晨钟1 王庆甫1△
1北京中医药大学第三附属医院(北京,100009)
Author(s):
CHEN Pu1 RUAN Anmin1 ZHOU Jun1 ZHANG Xiaozhe1 ZONG Chenzhong1 WANG Qingpu1△
1Beijing University of Chinese Medicine Third Affliated Hospital,Beijing 100009,China.
关键词:
通络止痛方;  凝胶外敷;  膝骨关节炎;  滑膜炎
Keywords:
Tongluo Zhitong;  gel external application;  knee osteoarthritis;  synovial inflammation
分类号:
R-33
文献标志码:
A
摘要:
目的:观察对比通络止痛方两种不同制剂对膝骨关节炎家兔模型滑膜炎的影响。方法:取32只成年雄性家兔随机分为4组,即空白对照组、膝骨关节炎(KOA)模型组、超声促透通络止痛方治疗组及通络止痛凝胶外敷组,每组8只。空白对照组不予处理,其余3组均予改良Hulth法建立KOA模型。8周后开始超声促透通络止痛方及外敷通络止痛凝胶治疗,每周治疗3次,连续治疗3周,随后处死家兔收集各组家兔血清、滑膜。使用ELISA法检测各组血清中IL-1β,IL-6,TNF-α及PGE2含量; qPCR及Western Blot(WB)法检测各组滑膜中IL-1β,IL-6,TNF-α及TLR4的表达量; 苏木精-伊红(HE)染色观察各组家兔滑膜炎性程度变化。结果:HE结果显示KOA模型组相较于空白对照组,其滑膜炎性浸润明显; 超声促透组与凝胶外敷组在治疗后滑膜炎性程度均有明显减轻,滑膜厚度减少。ELISA提示模型组血清中炎性致痛因子IL-1β,IL-6,TNF-α及PGE2显著提高,差异有统计学意义(P<0.01),而予以超声促透及凝胶外敷后均呈明显下降趋势,差异有统计学意义(P<0.05,P<0.01); qPCR及WB结果显示模型组IL-1β,IL-6,TNF-α及TLR4显著高表达,差异有统计学意义(P<0.01),而超声促透及凝胶外敷治疗可以显著逆转其表达,差异有统计学意义(P<0.05,P<0.01)。结论:通络止痛凝胶外敷可有效抑制KOA家兔滑膜炎,并与超声促透通络止痛方疗效相似,是治疗KOA的潜在治疗方式。
Abstract:
Objective:To observe and compare the effects of two different preparations of Tongluo Zhitong on synovial inflammation in rabbits knee osteoarthritis(KOA).Methods:Thirty-two adult male rabbits were randomly and averagely divided into the control group,the KOA model group,the ultrasound penetration Tongluo Zhitong recipe treatment groupand the Tongluo Zhitong gel external application group.Except for the control group,others were all established with the Hulth methodfor the KOA model.After 8 weeks,ultrasound penetration Tongluo Zhitong recipe treatment group and Tongluo Zhitong gel external application group were treated 3 times per week for 3 weeks.Then all rabbit serum and synoviumwere collected.ELISA method was used to detect the levels of IL-1β,IL-6,TNF-α and PGE2 in serum of each group; qPCR and WB methods were used to detect the expression of IL-1β,IL-6,TNF-α and TLR4 in synovium of each group.HE staining was used to check the degree of synovitis.Results:The HE results showed that the synovial inflammatory infiltration was more obvious in the KOA model group than that in the control group.The synovial inflammation was significantly reduced and the synovial thickness was reduced in the ultrasound penetration group and the gel external application group.ELISA showed that the serum inflammatory pain factors IL-1β,IL-6,TNF-α and PGE2 in the model group were significantly increased(P<0.01),and these cytokines were significantly decreased after ultrasound penetration and gel external application(P<0.01).qPCR and WB results showed that IL-1β,IL-6,TNF-α,and TLR4 were significantly overexpressed in the KOA model group(P<0.01),while ultrasound penetration and gel external application could significantly inhibit their expression(P<0.01).Conclusion:Tongluo Zhitong gel external application can effectively inhibit the inflammation of KOA rabbit synovium,and it is similar to the effect of ultrasonic penetration Tongluo Zhitong recipe,and is a potential treatment measure for KOA.

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备注/Memo

备注/Memo:
(收稿日期:2020-03-16)基金项目:国家自然科学基金面上项目(81373662) 北京中医药大学自主课题项目(2019-JYB-XS-232) △通信作者 E-mail:qingfu-wang@sohu.com
更新日期/Last Update: 2020-09-10