[1]李磊 吴昊 杨安忠 吴良绍△.鼠尾草提取物对类风湿性关节炎的治疗作用及其机制研究[J].中国中医骨伤科杂志,2020,28(09):6-11.
　LI Lei WU Hao YANG Anzhong WU Liangshao.The Effect and Mechanism of Salvia Japonica Thunb Extract on the Treatment of Rheumatoid Arthritis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2020,28(09):6-11.
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鼠尾草提取物对类风湿性关节炎的治疗作用及其机制研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第28卷
期数:
2020年09期
页码:
6-11
栏目:
实验研究
出版日期:
2020-09-10

文章信息/Info

Title:
The Effect and Mechanism of Salvia Japonica Thunb Extract on the Treatment of Rheumatoid Arthritis
文章编号:
1005-0205(2020)09-0006-06
作者:
李磊1 吴昊1 杨安忠1 吴良绍1△
1恩施土家族苗族自治州中心医院关节外科诊疗中心 (湖北 恩施,445000)
Author(s):
LI Lei1 WU Hao1 YANG Anzhong1 WU Liangshao1△
1Joint Surgery Diagnosis and Treatment Center,Enshi Tujia and Miao Autonomous Prefecture Central Hospital,Enshi 445000,Hubei China.
关键词:
鼠尾草提取物;  类风湿性关节炎;  炎症反应
Keywords:
Salvia japonica Thunb extract;  rheumatoid arthritis;  inflammatory response
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨鼠尾草提取物对类风湿性关节炎的治疗作用及其机制。方法:选择6个月龄SD大鼠,采用胶原诱导法建立类风湿关节炎(CIA)大鼠模型,并灌胃给予鼠尾草提取物(ST)治疗3周,测量大鼠的体质量变化、关节炎评分及踝关节肿胀度; 采用病理苏木精-伊红(HE)染色和番红固绿染色检测大鼠踝关节组织的病理变化; 采用酶联免疫吸附(ELISA)法检测大鼠外周血液中炎症因子白细胞介素(IL)-1β,IL-6和TNF-α的含量; 采用RT-PCR法检测大鼠滑膜组织中炎症因子IL-1β,IL-6和肿瘤坏死因子(TNF)-α的表达水平; 采用蛋白印迹法检测大鼠滑膜组织中TLR4,p-p65/p65和p-p50/p50的表达水平。结果:与模型组相比,ST组大鼠的体质量显著增加,且关节炎评分和关节肿胀度显著下降,差异有统计学意义(P<0.05); 同时,ST组大鼠的踝关节组织完整,软骨细胞排列规则且紧密,软骨基质损伤缓解,外周血液和滑膜组织中炎症因子IL-1β,IL-6和TNF-α的表达水平显著下调,差异有统计学意义(P<0.05); 除此之外,ST组大鼠关节滑膜组织中TLR4,p-p65/p65和p-p50/p50的表达水平显著下调,差异有统计学意义(P<0.05)。结论:鼠尾草提取物治疗类风湿性关节炎具有潜在价值,且这种作用可能与抑制TLR4-NF-κB信号介导的炎症反应有关。
Abstract:
Objective:To explore the effect and mechanism of Salvia japonica Thunb extract(ST)for the treatment of rheumatoid arthritis.Methods:Rheumatoid arthritis model were established by collagen induction method for 6-month-old SD rats selected.They were divided into model group,ST-L(200 mg/kg),ST-M(400 mg/kg),ST-H(800 mg/kg)and colchicine(MTX,1 mg/kg)group for and administered for 3 weeks.The changes of body weight,arthritis score and swelling of ankle joints were documented.Pathological changes in ankle joint tissues of rats were detected by pathological HE and Safranin O staining.Inflammatory factor interleukin(IL)-1β,IL-6 and tumor necrosis factor(TNF)-α in peripheral blood of rats were detected by enzyme-linked immunosorbent(ELISA)method.The expression of inflammatory factors IL-1β,IL-6 and tumor necrosis factor TNF-α were detected by RT-PCR.The expression of TLR4,p-p65/p65 and p-p50/p50 protein expression levels in rat synovial tissue were detected by Western Blotting.Results:Compared with the model group,the weight of rats in ST group significantly increased,and arthritis scores and joint swelling decreased significantly(P<0.05).Rats in the ST group showed intact ankle joint tissue sarranged regularly and tightness chondrocytes,and the cartilage matrix damage also relieved.The expression levels of inflammatory factors IL-1β,IL-6 and TNF-α in peripheral blood and synovial tissue were significantly down-regulated from ST group(P<0.05).In addition,the protein expression levels of the synovial membrane of the ST group rats TLR4,p-p65/p65 and p-p50/p50 were significantly down-regulated by treatment of ST(P<0.05).Conclusion:Salvia japonica Thunb extract ST has potential roles for the treatment of rheumatoid arthritis,and its effect may be related to the inhibition of TLR4-NF-κB signal-mediated inflammation.

参考文献/References:

[1] 郭锦晨,刘健,万磊,等.氧化应激及炎症反应在类风湿关节炎中的研究进展[J].风湿病与关节炎,2016,5(9):68-76.
[2] PISETSKY D S.Advances in the treatment of rheumatoid arthritis[J].NC Med J,2017,78(5):337-340.
[3] 元晓龙,曾晨,姚血明,等.中医药治疗类风湿关节炎的研究进展[J].贵阳中医学院学报,2018,40(3):89-100.
[4] 陈慕芝,吕蕾,照日格图.老年类风湿关节炎56例临床特点分析[J].风湿病与关节炎,2018,7(7):36-38.
[5] JONES G,NASH P,HALL S.Advances in rheumatoid arthritis[J].Med J Aust,2017,206(5):221-224.
[6] 白小荣,马岩,李旻辉,等.药用鼠尾草传统应用调查与研究进展[J].中国现代中药,2019,21(2):271-278.
[7] HALEAGRAHARA N,MIRANDA H S,ALIM M A,et al.Therapeutic effect of quercetin in collagen-induced arthritis[J].Biomed Pharmacother,2017,90:38-46.
[8] 朱健,武璐璐,张全书,等.枫杨乙醇提物对胶原诱导性关节炎大鼠的治疗作用及其机制研究[J].中国药理学通报,2019,35(1):117-123.
[9] DECKER E,MÜLLER-LADNER U.Rheumatoide arthritis[J].Der Internist,2013,54(4):434-440.
[10] 汪灿锋,叶正从,沈钦荣,等.五福饮含药血清对大鼠软骨细胞肿瘤坏死因子-α诱导凋亡软骨细胞活性及基质金属蛋白酶表达的影响[J].浙江中西医结合杂志,2019,29(9):718-723.
[11] 程苏云,陈香元,李洪伟,等.胶原诱导关节炎模型中髓系来源抑制细胞通过白细胞介素-1β促进Th17细胞分化的作用[J].中华临床免疫和变态反应杂志,2018,12(2):141-150.
[12] RANNIKKO E H,WEBER S S,KAHLE P J.Exogenous α-synuclein induces toll-like receptor 4 dependent inflammatory responses in astrocytes[J].Bmc Neuroscience,2015,16:57.
[13] 单佳铃,程虹毓,文乐,等.TLR/MyD88/NF-κB信号通路参与不同疾病作用机制研究进展[J].中国药理学通报,2019,35(4):451-455.
[14] KARIN M,DELHASE M.The I kappa B kinase(IKK)and NF-kappa B:key elements of proinflammatory signalling[J].Semin Immunol,2000,12(1):85-98.
[15] WANG J,HUANG J,WANG L,et al.Urban particulate matter triggers lung inflammation via the ROS-MAPK-NF-κB signaling pathway[J].J Thorac Dis,2017,9(11):4398-4412.
[16] 黄洁媛,刘文明.白细胞介素-4负调控NF-κB通路抑制炎症反应的机制研究[J].天津医药,2019,47(10):1025-1029.
[17] FULENWIDER H D,SMITH B M,NICHENKO A S,et al.Cellular and behavioral effects of lipopolysaccharide treatment are dependent upon neurokinin-1 receptor activation[J].J Neuroinflammation,2018,15(1):60.

备注/Memo

备注/Memo:
(收稿日期:2020-03-18)基金项目:湖北省卫生健康委中医药科研项目(ZY2019M077) △通信作者 E-mail:longtaodee@163.com
更新日期/Last Update: 2020-09-10