[1]贺志亮 王德成 李金学△ 顾骐 赵敏 靳骄 彭卓龙.腰痹颗粒治疗新西兰兔腰椎管狭窄症运动功能障碍机制初探[J].中国中医骨伤科杂志,2018,26(04):5-11.
　HE Zhiliang WANG Decheng LI Jinxue GU Qi ZHAO Min JIN Jiao PENG Zhuolong.Mechanism of Yaobitong Granule for Treating the Motor Dysfunction of Lumbar Spinal Canal Stenosis in New Zealand Rabbits[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2018,26(04):5-11.
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腰痹颗粒治疗新西兰兔腰椎管狭窄症运动功能障碍机制初探()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第26卷
期数:
2018年04期
页码:
5-11
栏目:
实验研究
出版日期:
2018-04-10

文章信息/Info

Title:
Mechanism of Yaobitong Granule for Treating the Motor Dysfunction of Lumbar Spinal Canal Stenosis in New Zealand Rabbits
文章编号:
1005-0205(2018)04-0005-07
作者:
贺志亮1 王德成1 李金学2△ 顾骐2 赵敏2 靳骄2 彭卓龙2
1北京市通州区中西医结合医院(北京,101149) 2中国中医科学院望京医院
Author(s):
HE Zhiliang1 WANG Decheng1 LI Jinxue2△ GU Qi2 ZHAO Min2 JIN Jiao2 PENG Zhuolong2
1Beijing Tongzhou District Hospital of Integrated Traditional Chinese and Western Medicine,Beijing 101149,China; 2Wangjing Hospital of Chinese Academy of Chinese Medicine,Beijing 100102,China.
关键词:
腰痹颗粒;  腰椎管狭窄症;  运动功能障碍;  作用机制;  动物实验
Keywords:
Keywords: yaobitong decoction;  lumbar spinal stenosis;  dyskinesia;  mechanism;  animal experiment
分类号:
R-33
文献标志码:
A
摘要:
目的:通过对新西兰兔腰椎管狭窄动物模型BBB评分,血清软骨寡聚基质蛋白(COMP)、白介素-1β(IL-1β)、肿瘤坏死因子(TNF-α)和关节突及椎板病理形态学观察,探讨腰痹汤治疗腰椎管狭窄症的作用机制。方法:取3月龄清洁级新西兰兔48只,体质量(2.80±0.75)kg,按体质量分层随机分组的方法将新西兰大兔分为空白组(A组)、模型组(B组)、实验药物组(C组)及阳性药物对照组(D组),每组12只,采用椎板间开窗、自体骨回填和加压缝合的方式制作腰椎管狭窄症动物模型。于术后2,4,8及12周采用 BBB 运动功能评分法进行行为学观察,各组各时间点分别处死1,1,4及6只新西兰兔,并取L4和L5左侧关节突关节,L5椎板行病理学观察。术后8周确认手术成功,A组和B组给予蒸馏水灌胃,C组和D组分别给予腰痹颗粒、丹鹿通督片混悬液灌胃,于术后8周和12周分别取兔耳缘血,观察用药前后血清COMP,IL-1β和TNF-α浓度变化。结果:BBB评分显示,各时间点A组差异均无统计学意义(P>0.05),B,C及D 3组于术后2,4及8周呈逐渐下降趋势,3组各时间点组间差异无统计学意义(P>0.05),但与A组相比,3组各时间点组间差异有统计学意义(P<0.05); 术后12周,除B组外,C组和D组较术后8周均有提高,差异有统计学意义(P<0.05),但组间差异无统计学意义(P>0.05); 关节突关节和椎板病理切片显示,术后2,4及8周,B,C及D 3组显示关节突软骨不平整,伴有缺损和溃疡面,大量软骨细胞坏死,胶原纤维增生明显,椎板骨小梁破坏,椎板与硬膜囊粘连,伴有大量炎症细胞浸润; 术后12周,C组和D组可见较多新生的骨小梁形成且成骨活跃,炎症细胞浸润减少; 血清学示,术后8周,B,C及D 3组血清COMP,IL-1β及TNF-α浓度均高于A组,差异有统计学意义(P<0.05),但组间差异无统计学意义(P>0.05); 术后12周与术后8周相比,除B组差异无统计学意义(P>0.05),C组和D组血清COMP,IL-1β及TNF-α浓度均有降低,差异有统计学意义(P<0.05),组间比较C组COMP浓度低于D组,差异有统计学意义(P<0.05),而IL-1β和TNF-α浓度则差异无统计学意义(P>0.05)。结论:通过降低血清中COMP的浓度,从而改善关节突软骨功能,抑制IL-1β及TNF-α炎性表达可能是腰痹汤治疗腰椎管狭窄症的机制之一。
Abstract:
Abstract Objective: Based on the observing the BBB score,serum bone oligomeric matrix protein(COMP),interleukin 1 beta(IL-1β),tumor necrosis factor alpha(TNF-α),and the pathomorphology of the articular process and vertebral lamina in animal model of lumbar spinal canal stenosis in New Zealand rabbits,to detective the mechanism of Yaobitong decoction for which disease.Methods:Forty-eight 3 months old clean New Zealand rabbits were weighted(2.80±0.75)kg.They were divided into 4 groups according to weight stratification random grouping method:control group(A group),model group(B group),experimental group(C group),positive control group(group D),12 rats in each group.The animal model of lumbar spinal stenosis was established by the methods of interlaminar opening,autogenous bone backfilling and pressure suture.The behavioral assessment was performed by BBB motor function score method at time point of 2nd,4th,8th and 12th weeks after surgery.And at this time point,1,1,4 and 6 rabbits were sacrificed.The L4～5 facet joints and L5 laminae were taken for pathological observation.The model was conformed 8 weeks after operation.The animal in A,B groups were gavage with distilled water,and C,D group were given Yaobitong granule and Danlutongdu tablet suspension respectively.The blood of rabbit ears was collected at 8th and 12th weeks after surgery,and the changes of serum COMP,IL-1β and TNF-α were observed before and after treatment.Results:There was no statistically significant difference in group A at different time point in BBB score(P>0.05),but there showed reduced trend in B,C,and D group at time point of 2nd,4th,and 8th weeks after surgery,and there was significant difference compared with group A(P<0.05).The BBB score in C and D group were increased at 12th weeks after surgery(P<0.05),and there was no significant difference between the two groups(P>0.05).The pathological section of facet joints and laminae showed the joint cartilage was defected,with a large number of ulcer surface,cartilage necrosis,collagen fiber hyperplasia,vertebral trabecular bone destruction,lamina and epidural adhesion,accompanied by inflammatory cell infiltration in B,C,and D group at time point of 2nd,4th,and 8th weeks after surgery.The pathological section in C and D group showed more new bone formation,less infiltration of inflammatory cells at 12th weeks after surgery.The concentration of COMP,IL-1β and TNF-α in B,C and D group were significantly higher than group A(P<0.05),but there was no statistical difference between each the three groups(P>0.05).The concentration of COMP,IL-1β and TNF-α in C and D group were decreased at 12th weeks after surgery(P<0.05),and the concentration of COMP in group C was lower than group D(P<0.05),the concentration of IL-1β and TNF-α in C and D group were no significant difference(P>0.05).Conclusion:By decreasing the concentration of COMP in serum and improving the function of articular cartilage,inhibiting the inflammatory expression of IL-1β and TNF-α may be one of the mechanisms of Yaobitong decoction for treating the lumbar spinal stenosis.

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更新日期/Last Update: 2018-04-15