[1]王雷 王孝辉△ 张海龙 张彩丽 丁强.不同剂量骨碎补总黄酮对大鼠Masquelet技术诱导膜中BMP-2和VEGF表达的影响[J].中国中医骨伤科杂志,2018,26(04):1-4.
　WANG Lei WANG Xiaohui ZHANG Hailong ZHANG Caili DING Qiang.Effects of Different Dose of Rhizoma Drynariae on Expression of BMP-2 and VEGF in Membrane of the Rats Induced by Masquelet Technology[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2018,26(04):1-4.
点击复制

不同剂量骨碎补总黄酮对大鼠Masquelet技术诱导膜中BMP-2和VEGF表达的影响()
分享到：

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第26卷
期数:
2018年04期
页码:
1-4
栏目:
实验研究
出版日期:
2018-04-10

文章信息/Info

Title:
Effects of Different Dose of Rhizoma Drynariae on Expression of BMP-2 and VEGF in Membrane of the Rats Induced by Masquelet Technology
文章编号:
1005-0205(2018)04-0001-04
作者:
王雷1 王孝辉2△ 张海龙2 张彩丽2 丁强2
1湖南中医药大学(长沙,410000) 2河南省洛阳正骨医院(河南省骨科医院)
Author(s):
WANG Lei1 WANG Xiaohui2△ ZHANG Hailong2 ZHANG Caili2 DING Qiang2
1Hunan University of Traditional Chinese Medicine,Changsha 410000,China; 2Luoyang Orthopedic Hospital of Henan Province/Orthopedic Hospital of Henan Province,Luoyang 471002,Henan China.
关键词:
骨碎补总黄酮;  诱导膜;  Masquelet技术;  骨形成蛋白-2;  血管内皮生长因子
Keywords:
Keywords: total flavonoids of rhizoma drynariae;  induced membrane;  masquelet technique;  BMP-2;  vascular endothelial growth factor(VEGF)
分类号:
R-33
文献标志码:
A
摘要:
目的:观察不同剂量的骨碎补总黄酮对大鼠Masquelet技术诱导膜中骨形成蛋白-2(Bone Morphogenetic Protein,BMP-2)和血管内皮生长因子(Vascular Endothelial Growth Factor,VEGF)表达的影响。方法:选取48只3月龄SD大鼠,雌雄不限,按随机数字表法分为4组:空白对照组、低剂量组、中剂量组及高剂量组,每组12只。所有大鼠均建立左侧胫骨长段骨缺损模型,并予以抗生素骨水泥填塞。术后低、中、高剂量组分别给予骨碎补总黄酮67.5,202.5和607.5 mg/(kg·d)灌胃,空白对照组给予等量蒸馏水灌胃,连续灌胃6周后取出骨水泥,切取诱导膜,检测诱导膜中BMP-2和VEGF的表达情况。结果:灌胃6周后,各组BMP-2和VEGF表达组间比较,差异有统计学意义(P<0.05); 各组两两比较,低、中、高剂量组BMP-2和VEGF表达均显著高于空白对照组,且高剂量组表达最明显,中剂量组次之,低剂量组再次之,呈剂量依赖关系,差异均有统计学意义(P<0.05)。结论:不同剂量的骨碎补总黄酮均可促进大鼠Masquelet技术诱导膜中BMP-2和VEGF的表达,且呈剂量依赖关系,高剂量组最明显。
Abstract:
Abstract Objective:To observe the effects of different dose of rhizoma drynariae on the expression of bone morphogenetic protein(BMP-2)and vascular endothelial growth factor(VEGF)in membrane of rat which induced by masquelet technology.Methods: Forty-eight SD rats without limitation of sex were randomly divided into four groups:control group,low dose group,middle dose group and high dose group.All rats were established with long tibial long bone defect model,and antibiotic bone cement was filled.The low,medium and high dose groups were given 67.5,202.5 and 607.5 mg/(kg·d)of the total flavonoids.The expression of BMP-2 and VEGF in the induced membrane and the growth of the blood vessel were measured after 6 weeks of continuous administration.Results: After 6 weeks of intragastric administration,the expression of BMP-2 and VEGF had compared each other,the difference were statistically significant(P<0.05).All groups multiple comparison,the expression of BMP-2 and VEGF in the low,middle and high dose groups was significantly higher than that in the blank control group.And the highest expression was in the high dose group,the second was in the middle dose group,and the third was in the low dose group.There was a dose-dependent relationship,and the differences were statistically significant(P<0.05).Conclusion: Different doses of total flavonoids of rhizoma drynariae can promote the expression of BMP-2 and VEGF in membrane of rat which induced by masquelet technology in a dose-dependent manner.

参考文献/References:

[1] Masquelet AC,Fitoussi F,Begue T,et al.Reconstruction of the long bones by the induced membrane and spongy autograft[J].Ann Chir Plast Esthet,2000,45(3):346-353.
[2] Han W,Shen J,Wu H,et al.Induced membrane technique:advances in the management of bone defects[J].Int J Surg,2017,42:110-116.
[3] 钱茜.骨碎补化学成分和药理作用研究进展[J].中国生化药物杂志,2015,35(3):186-188.
[4] 赵文博,刘雷.长骨大段骨缺损治疗方式的研究进展[J].现代临床医学,2014,40(3):230-232.
[5] 张开,高尚武,田力升,等.探讨胫骨创伤后节段性骨缺损治疗方法[J].中外医学研究,2011,9(19):110-111.
[6] 殷渠东,孙振中,顾三军,等.骨搬运与骨短缩-延长治疗胫骨骨缺损合并软组织缺损的疗效比较[J].中国修复重建外科杂志,2014,28(7):818-822.
[7] 王振山,程鑫葵,侯福山,等.诱导膜技术治疗大段骨缺损的研究进展[J].实用骨科杂志,2015,21(2):140-144.
[8] 殷渠东,孙振中,顾三军.应用Masquelet技术修复骨缺损的研究进展[J].中国修复重建外科杂志,2013,27(10):1273-1276.
[9] Rezzouk J,Leclerc J,Leger O,et al.Bone reconstruction with induced membranes and cancellous autograft:results in 18 cases of osteitis[J].J Bone Joint Sung Br,2005,113(4):235.
[10] Biau DJ,Pannier S,Masquelet AC,et al.Case report:reconstruction of a 16-cm diaphyseal defect after Ewing's resection in a child[J].Clin Orthop Relat Res,2009,467(2):572-577.
[11] Masquelet AC,Begue T.The concept of induced membrane for reconstruction of long bone defects[J].Orthop Clin North Am,2010,41(1):27-37.
[12] Henrich D,Seebach C,Nau C,et al.Establishment and characterization of the Masquelet induced membrane technique in a rat femur critical-sized defect model[J].J Tissue Eng Regen Med,2016,10(10):E382-E396.
[13] Pelissier P,Masquelet AC,Bareille R,et al.Induced membranes secrete growth factors including vascular and osteoinductive factors and could stimulate bone regeneration[J].J Orthop Res,2004,22(1):73-79.
[14] Klaue K,Knothe U,Anton C,et al.Bone regeneration in long-bone defects:tissue compartmentalisation? In vivo study on bone defects in sheep[J].Injury,2009,40(Suppl 4):S95-102.
[15] 上官文姬,李鹤,汤璐敏.骨碎补总黄酮对大鼠成骨细胞VEGF和FGF-2表达的影响[J].辽宁中医药大学学报,2014,16(8):38-41.
[16] 张攀,白钰,李云裳,等.BMP-2在骨组织再生和修复上的作用研究进展[J].中国实用医药,2011,6(20):226-227.
[17] 董福慧,郑军,程伟.骨碎补对骨愈合过程中相关基因表达的影响[J].中国中西医结合杂志,2003,23(7):518-521.
[18] 高怡加,黄培镇,李悦,等.骨碎补总黄酮对牵张成骨过程中骨形态发生蛋白-2和转化生长因子-β1表达的影响[J].广州中医药大学学报,2016,33(5):679-683.

备注/Memo

备注/Memo:
基金项目:河南省中医药科学研究专项课题资助项目 (2015ZY02062) △通信作者 E-mail:963099784@qq.com
更新日期/Last Update: 2018-04-15