[1]陈世宣 冯伟△ 卢远坚 李安琪 张增乔 刘益杰 曹彦俊.牛蒡子苷元对体外培养软骨细胞Ⅱ型胶原表达的影响[J].中国中医骨伤科杂志,2017,25(10):6-10.
　CHEN Shixuan FENG Wei LU Yuanjian LI Anqi ZHANG Zengqiao LIU Yijie CAO Yanjun.Expression of Collagen Ⅱ mRNA for Arctigenin on Chondrocytes In Vitro[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2017,25(10):6-10.
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牛蒡子苷元对体外培养软骨细胞Ⅱ型胶原表达的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第25卷
期数:
2017年10期
页码:
6-10
栏目:
实验研究
出版日期:
2017-10-15

文章信息/Info

Title:
Expression of Collagen Ⅱ mRNA for Arctigenin on Chondrocytes In Vitro
文章编号:
1005-0205(2017)10-0006-05
作者:
陈世宣1; 2 冯伟1; 3△ 卢远坚1 李安琪1 张增乔1 刘益杰1 曹彦俊1
1上海中医药大学(上海,201203) 2温州市中西医结合医院(温州,325000) 3上海市第七人民医院(上海,200137) △通信作者 E-mail:fwginger@126.com
Author(s):
CHEN Shixuan1; 2 FENG Wei1; 3△ LU Yuanjian1 LI Anqi1 ZHANG Zengqiao1 LIU Yijie1 CAO Yanjun1
1Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China; 2Wenzhou City Hospital of Integrated Traditional Chinese and Western Medicine,Wenzhou 325000,Zhejiang China; 3Shanghai 7th People's Hospital,Shanghai 200137,China.
关键词:
膝骨性关节炎;  软骨细胞;  牛蒡子苷元;  Ⅱ型胶原;  基质金属蛋白酶-13
Keywords:
Keywords: knee osteoarthritis;  chondrocyte;  arctigenin;  collagen Ⅱ;  matrix metalloproteinases-13
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨牛蒡子苷元(Arctigenin,ARC-G)对软骨细胞的增殖,及对Ⅱ型胶原(Collagen Ⅱ,ColⅡ)、Y染色体性别决定相关高泳动蛋白盒基因9(SRY-related high mobility group-box gene 9,SOX9)和基质金属蛋白酶-13(Matrix Metalloproteinase-13,MMP-13)表达的影响。方法:体外分离培养软骨细胞,取第1代细胞进行实验干预。实验分为空白组(ARC-0组)、低浓度牛蒡子苷元组(ARC-L组)和高浓度牛蒡子苷元组(ARC-H组)。各组分别予相应干预措施处理48 h后,以SABC免疫细胞化学染色法观察ColⅡ的表达情况; 以CCK-8法检测软骨细胞的增殖情况; 以Real-time quantitative PCR检测ColⅡ,SOX9及MMP-13 mRNA的表达情况; 以酶联免疫吸附法(ELISA)检测细胞培养上清液中ColⅡ的含量。结果:1)ARC-L组和ARC-H组的ColⅡ阳性染色强度均强于ARC-0组,其中ARC-H组染色最强; 2)与ARC-0组相比,在不同时间点ARC-L组和ARC-H组均可促进软骨细胞的增殖,且ARC-H组促增殖作用更显著,除24 h和96 h的ARC-L组外其余各时间点差异均有统计学意义(P<0.05); 3)与ARC-0组相比,ARC-L组和ARC-H组均可上调ColⅡ和SOX9 mRNA的表达,下调MMP-13 mRNA的表达,且ARC-H组作用更为显著,除ARC-L组ColⅡ及MMP-13 mRNA外,差异均有统计学意义(P<0.05); 4)与ARC-0组相比,ARC-L组和ARC-H组均可促进ColⅡ蛋白的表达,且ARC-H组作用更为显著,除ARC-0组和ARC-L组外,差异均有统计学意义(P<0.05)。结论:牛蒡子苷元可通过上调SOX9和ColⅡmRNA的表达,下调MMP-13 mRNA的表达以促进ColⅡ蛋白的合成。
Abstract:
Abstract Objective:To detect the effect of arctigenin on chondrocytes proliferation,and on expression of Collagen Ⅱ,SRY-related highmobility group-box gene 9(SOX 9),and matrix metallopr-oteinase-13(MMP-13)mRNA.Methods:Chondrocytes were isolated and then cultured in vitro,the first generation of chondrocytes were used for experimental intervention.The chondrocytes were divided into blank group(ARC-0 group),low concentration of arctigenin group(ARC-L group),and high concentration of arctigenin group(ARC-H group).The chondrocytes in each group were respectively treated for 48 h by the corresponding intervention methods,then the expression of Col Ⅱ was observed by immunocytochemisty SABC method.The chondrocytes proliferation was detected by Cell counting kit-8(CCK8)method.The expression of Col Ⅱ,SOX 9,and MMP-13 mRNA were detected by real-time quantitative PCR.And the collagen Ⅱ content of supernatants was detected by enzyme-linked immunosorbent assay(ELISA).Results:1)The positive staining potency of ARC-L group and ARC-H group were stronger than ARC-0 group,and ARC-H group was the strongest one.2)The ARC-L group and the ARC-H group could promote chondrocytes proliferation at different point of time compared with ARC-0 group,and the ARC-H group was more significantly,the optical density were significantly different at other all-time points except the 24 h and 96 h point(P<0.05).3)Compared with ARC-0 group,both of the ARC-L group and the ARC-H group were able to up-regulate the expression of Col Ⅱ and SOX9 mRNA,and down-regulate the expression MMP-13 mRNA,and the ARC-H group was more significantly.There was significantly different(P<0.05)expected expression of Col Ⅱ and MMP-13 mRNA in ARC-L group.4)Compared with ARC-0 group,the content of collagen Ⅱ in both ARC-L group and ARC-H group were upregulated,and ARC-H group was more significantly.Expect for ARC-0 group and ARC-L group,the difference among other group was significantly different(P<0.05).Conclusion:Arctigenin can promote the protein expression of Col Ⅱ by up-regulating the expression of Col Ⅱ and SOX 9 mRNA,and down-regulating the expression of MMP-13 mRNA.

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备注/Memo

备注/Memo:
基金项目:上海市自然基金资助项目(15ZR1441000) 上海市卫生和计划生育委员会中医药科研课题 (2016LP037) 上海市浦东新区卫计委卫生科技资助项目 (PW2014A-20)
更新日期/Last Update: 1900-01-01