[1]苏新平 朱克俭△ 谭旭仪 何灿宇 黄刚 张堃.止痛健骨方对兔膝骨关节炎软骨IKKα及NF-κB mRNA的影响[J].中国中医骨伤科杂志,2017,25(10):1-5.
　SU Xinping ZHU Kejian TAN Xuyi HE Canyu HUANG Gang ZHANG Kun.Expression of IKKα and NF-κB mRNA for Zhitongjiangu Recipe on Cartilage of Knee Osteoarthritis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2017,25(10):1-5.
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止痛健骨方对兔膝骨关节炎软骨IKKα及NF-κB mRNA的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第25卷
期数:
2017年10期
页码:
1-5
栏目:
实验研究
出版日期:
2017-10-15

文章信息/Info

Title:
Expression of IKKα and NF-κB mRNA for Zhitongjiangu Recipe on Cartilage of Knee Osteoarthritis
文章编号:
1005-0205(2017)10-0001-05
作者:
苏新平1 朱克俭1△ 谭旭仪1 何灿宇1 黄刚2 张堃3
1湖南省中医药研究院附属医院(长沙,410006) 2湖南中医药大学 3湖南中医药大学第一附属医院 △通信作者 E-mail:zkjo0731@263.net
Author(s):
SU Xinping1 ZHU Kejian1△ TAN Xuyi1 HE Canyu1 HUANG Gang2 ZHANG Kun3
1Affiliated Hospital of Hunan Academy of Chinese Medical Science,Changsha 410006,China; 2Hunan University of Traditional Chinese Medicine,Changsha 410208,China; 3The First Hospital,Affiliated to Hunan University of Traditional Chinese Medicine,Changsha 410007,China.
关键词:
止痛健骨方;  兔;  膝骨关节炎;  IκB激酶α;  核因子κB
Keywords:
Keywords: Zhitongjiangu recipe;  rabbit;  knee osteoarthritis;  IKKα;  NF-κB
分类号:
R-33
文献标志码:
A
摘要:
目的:观察止痛健骨方对兔膝骨关节炎软骨IKKα及NF-κB mRNA的影响。方法:将90只白兔随机分为正常对照组、模型对照组、阳性对照组、止痛健骨低剂量组、止痛健骨高剂量组,每组各18只,采用木瓜蛋白酶制备兔膝骨关节炎模型,止痛健骨低剂量组、止痛健骨高剂量组分别给与止痛健骨方低剂量、高剂量药物灌胃,阳性对照组给与盐酸氨基葡萄糖片灌胃,用量0.067 2 g/kg,正常对照组和模型对照组则给与生理盐水灌胃。在模型组、实验组灌胃第4周、第8周三个时间点,各组随机选择6只白兔,检测软骨IKKα及NF-κB mRNA表达水平,进行对比分析。结果:在实验干预前、实验干预第4周,与正常对照组比较,模型对照组、阳性对照组、止痛健骨低剂量组、止痛健骨高剂量组软骨IKKα及NF-κB mRNA表达增加,差异有统计学意义(P<0.05)。与模型对照组比较,阳性对照组、止痛健骨低剂量组、止痛健骨高剂量组第4周、第8周软骨IKKα及NF-κB mRNA表达下降,差异有统计学意义(P<0.05)。结论:止痛健骨方能降低兔KOA模型关节软骨IKKα及NF-κB mRNA表达,这可能为其治疗KOA的作用机理。
Abstract:
Abstract Objective: To observe the expression of IKKα and NF-κB mRNA for Zhitongjiangu recipe on cartilage of knee osteoarthritis(KOA).Methods: All 90 rabbits were randomly divided into normal control group,model control group,positive control group,Zhitongjiangu low dose group,and Zhitongjiangu high dose group,with 18 rats in each group.The rabbit model of KOA was successfully established by papain intra-articular injection.The rabbits in Zhitongjiangu low dose group and high dose group were give corresponding Zhitongjiangu recipe intragastric administration.The positive control group were given hydrochloride glucose tablets orally; and the normal control group and model control group were intragastrically administrated with the same dose of normal saline.The expression of IKKα and NF-κB mRNA in cartilage were detected at baseline and at 4th weeks and 8th weeks after administrating.Results: The expression of IKKα and NF-κB mRNA of model control group,positive control group,Zhitongjiangu low dose group,and Zhitongjiangu high dose group were significantly increased compared with the normal control group at baseline and at 4th weeks after administrating(P<0.05).The expression of IKKα and NF-κB mRNA of positive control group,Zhitongjiangu low dose group,and Zhitongjiangu high dose group were significantly decreased compared with the model control group at baseline and at 4th weeks and 8th weeks after administrating(P<0.05).Conclusion: Zhitongjiangu recipe can decrease the expression of IKKα and NF-κB mRNA on cartilage of KOA,which may be the mechanism for treating KOA.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(81603482) 湖南省自然科学基金项目(2016JJ115) 湖南省中医药科研基金资助项目(2008035)
更新日期/Last Update: 1900-01-01