[1]李志强 王少华△ 魏瑄 白玉 李玲 侯颖周 骆晓飞 乔登朝.活络骨康丸家兔含药血清抑制酒精诱导骨髓基质细胞成脂分化的实验研究[J].中国中医骨伤科杂志,2017,25(03):6-9.
　LI Zhiqiang WANG Shaohua WEI Xuan BAI Yu LI Ling HOU Yingzhou LUO Xiaofei QIAO Dengchao.Inhibitory Effect of Huoluogukang Pill Drug-containing Serum in Rabbits on Adipogenic Differentiation of Bone Marrow Stromal Cells Induced by Alcohol[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2017,25(03):6-9.
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活络骨康丸家兔含药血清抑制酒精诱导骨髓基质细胞成脂分化的实验研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第25卷
期数:
2017年03期
页码:
6-9
栏目:
实验研究
出版日期:
2017-03-08

文章信息/Info

Title:
Inhibitory Effect of Huoluogukang Pill Drug-containing Serum in Rabbits on Adipogenic Differentiation of Bone Marrow Stromal Cells Induced by Alcohol
文章编号:
1005-0205(2017)03-0006-04
作者:
李志强1 王少华2△ 魏瑄2 白玉2 李玲2 侯颖周2 骆晓飞2 乔登朝1
1.河南中医药大学研究生院(郑州,450008)
2.郑州市骨科医院
△.通信作者 E-mail:wangshaohua1719@163.com
Author(s):
LI Zhiqiang1 WANG Shaohua2△ WEI Xuan2 BAI Yu2 LI Ling2 HOU Yingzhou2 LUO Xiaofei2 QIAO Dengchao1
1.Henan University of Traditional Chinese Medicine,Zhengzhou 450008,China;
2.Zhengzhou Orthopaedic Hospital,Zhengzhou 450052,China.
关键词:
活络骨康丸;  防治;  酒精;  股骨头坏死;  成脂分化
Keywords:
Huoluogukang pill;  prevent;  alcohol;  osteonecrosis of femoral head;  adipogenetic differentiation
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨活络骨康丸家兔含药血清抑制酒精诱导骨髓基质细胞(MSCs)成脂分化作用。方法:给予新西兰大白兔2 mL/kg活络骨康丸连续灌胃7 d后,制备含药血清; 随机法将体外分离培养的兔MSCs分3组,对照组给予含10%正常家兔血清的DMEM培养液; 模型组给予含10%正常家兔血清的DMEM培养液及0.09 mol/L酒精处理细胞; 实验组给予含10%活络骨康丸血清的DMEM培养液及0.09 mol/L酒精处理细胞。采用苏丹Ⅲ染色法脂染各组细胞,光镜下计数脂肪细胞个数; 对细胞内甘油三酯含量、细胞培养液中骨钙素含量和胞内碱性磷酸酶活性进行测定。结果:实验组脂肪细胞数量及胞内甘油三脂含量均明显低于模型组,差异有统计学意义(P<0.001),与对照组间差异无统计学意义(P>0.05)。实验组测得碱性磷酸酶活性及骨钙素含量均明显高于模型组,差异有统计学意义(P<0.001),与对照组间差异无统计学意义(P>0.05)。结论:活络骨康丸家兔含药血清能抑制酒精诱导MSCs分化为脂肪细胞,维持其成骨分化,对抗酒精的毒性作用,对酒精性股骨头坏死具有防治作用。
Abstract:
Objective:To study the effect of Huoluogukang pill drug-containing serum on inhibiting adipogenic differentiation of bone marrow stromal cells(MSCs)induced by alcohol.Methods:The New Zealand white rabbits were intragastrically administered with 2 mL/kg Huoluogukang pill for 7 days to prepare drug-containing serum.The rabbits MSCs isolated and cultured in vitro were randomly divided into 3 groups as follows:the control group was given DMEM medium containing 10% normal rabbit serum,the model group was treated with DMEM medium containing 10% normal rabbit serum and 0.09mol/L alcohol,and the experimental group was treated with DMEM medium containing 10% Huoluogukang pill serum and 0.09 mol/L alcohol.The cells in each group were stained with Sudan Ⅲ,the number of adipocytes was counted under light microscope.The content of intracellular triglyceride,the content of osteocalcin in cell culture medium and the activity of intracellular alkaline phosphatase were measured.Results:The number of adipocytes and intracellular triglyceride in the experimental group were significantly lower than those in the model group,there was statistically significant difference(P<0.001),while there was no statistically significant difference between the experimental group and the control group(P>0.05).The activity of alkaline phosphatase and the content of osteocalcin were significantly higher in the experimental group than the model group(P<0.001),while there was no statistically significant difference between the experimental group and the control group(P>0.05).Conclusion:Drug-containing serum with Huoluogukang pill in rabbits can inhibit the adipogenetic differentiation of MSCs induced by alcohol,maintain their osteogenic differentiation,and against the toxic effects of alcohol on alcoholic.And it has a preventive effect on alcohol-induced osteonecrosis of femoral head.

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备注/Memo

备注/Memo:
基金项目:郑州市科技攻关项目(112PPTSF309-5)
更新日期/Last Update: 2017-03-15