[1]唐成坤 潘浩 王栋 林士明 陶其杰 贾高永 朱杭△.强筋壮骨祛风合剂对腰椎间盘退变AQP1和AQP5表达的干预作用[J].中国中医骨伤科杂志,2017,25(03):1-5.
　TANG Chengkun PAN Hao WANG Dong LIN Shiming TAO Qijie JIA Gaoyong ZHU Hang.Effect of Qiangjinzhuangguqufeng Mixture on the Expression of AQP1 and AQP5 in Lumbar Intervertebral Disc Degeneration[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2017,25(03):1-5.
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强筋壮骨祛风合剂对腰椎间盘退变AQP1和AQP5表达的干预作用()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第25卷
期数:
2017年03期
页码:
1-5
栏目:
实验研究
出版日期:
2017-03-08

文章信息/Info

Title:
Effect of Qiangjinzhuangguqufeng Mixture on the Expression of AQP1 and AQP5 in Lumbar Intervertebral Disc Degeneration
文章编号:
1005-0205(2017)03-0001-05
作者:
唐成坤1 潘浩1 王栋1 林士明1 陶其杰1 贾高永1 朱杭1△
1.浙江中医药大学附属广兴医院(杭州,310007)
△.通信作者 E-mail:13958015488@163.com
Author(s):
TANG Chengkun1 PAN Hao1 WANG Dong1 LIN Shiming1 TAO Qijie1 JIA Gaoyong1 ZHU Hang1△
1.Guangxing Hospital,Affiliated to Zhejiang University of Traditional Chinese Medicine,Hangzhou 310007,China.
关键词:
腰椎间盘退变;  强筋壮骨祛风合剂;  水蛋白通道1;  水蛋白通道5
Keywords:
lumbar disc degeneration;  Qiangjinzhuangguqufeng mixture;  aguaporin1(AQP1);  aguaporin5(AQP5)
分类号:
R-33
文献标志码:
A
摘要:
目的:观察强筋壮骨祛风合剂对腰椎间盘退变水通道蛋白1(AQP1)和水通道蛋白5(AQP5)表达的干预作用。方法:将新西兰大白兔腰椎穿刺建立腰椎间盘退变模型,用RT-PCR 及免疫组化检测 AQP1和AQP5在椎间盘组织中的表达,观察模型大白兔给药6周后的变化。结果:1)RT-PCR检测:(1)各组AQP1表达比较,假手术组与空白对照组差异无统计学意义(P>0.05),模型空白组、腰痛宁组、强筋壮骨祛风合剂组与空白对照组差异有统计学意义(P<0.05),空白对照组、假手术组、腰痛宁组、强筋壮骨祛风合剂组与模型空白组差异有统计学意义(P<0.05)。(2)各组AQP5表达比较,假手术组与空白对照组差异无统计学意义(P>0.05),模型空白组、腰痛宁组、强筋壮骨祛风合剂组与空白对照组差异有统计学意义(P<0.05),空白对照组、假手术组、腰痛宁组、强筋壮骨祛风合剂组与模型空白组差异有统计学意义(P<0.05)。2)免疫组化试剂检测:(1)各组AQP1表达比较,造模成功后,模型空白组椎间盘上AQP1阳性面积与空白对照组、假手术组差异有统计学意义(P<0.05),空白对照组和假手术组差异无统计学意义(P>0.05),其余组之间差异均有统计学意义(P<0.05)。(2)各组AQP5表达比较,造模成功后,模型空白组椎间盘上AQP5阳性面积与空白对照组、假手术组差异有统计学意义(P<0.05),空白对照组和假手术组差异无统计学意义(P>0.05),其余组之间差异均有统计学意义(P<0.05)。结论:强筋壮骨祛风合剂对 AQP1及AQP5的调节作用是其疗效机制之一,具有改善腰椎间盘退变的作用。
Abstract:
Objective:To observe the intervention mechanism of Qiangjinzhuangguqufeng mixture on the expression of aquaporin1(AQP1)and aquaporin5(AQP5)in lumbar intervertebral disc degeneration.Methods:The lumbar intervertebral disc degeneration model was established by lumbar puncture in New Zealand white rabbits.The expression of AQP1 and AQP5 in intervertebral disc was detected by RT-PCR and immunohistochemistry.The changes of the model rabbits were observed 6 weeks after the administration.Results:1)RT-PCR detection:(1)The expression of AQP1 in each group was compared.There was no difference in the expression of AQP1 between the sham operation group and the blank control group(P>0.05).There were difference in the expression of AQP1 among the model blank group,the Yaotongning group,the Qiangjinzhuangguqufeng mixture group and the blank control group(P<0.05).(2)And there were difference among the blank control group,the sham operation group,the Yaotongning group,the Qiangjinzhuangguqufeng mixture group and the model blank group(P<0.05).The expression of AQP5 in each group was compared.There was no difference in the expression of AQP5 between the sham operation group and the blank control group(P>0.05).There were difference among the model blank group,the Yaotongning group,the Qiangjinzhuangguqufeng mixture group and the blank control group(P<0.05),and there were differences among the blank control group,the sham operation group,the Yaotongning group,the Qiangjinzhuangguqufeng mixture group and the model control group(P<0.05).2)Immunohistochemically detection:(1)The expression of AQP1 in each group was compared.After successful modeling,the positive area of AQP1 in intervertebral discs in the model blank group was different from that in the blank control group and the sham operation group(P<0.05),but there was no difference between the blank control group and the sham operation group(P>0.05),the rest had statistically significant difference(P<0.05).The expression of AQP5 in each group was compared.Compared with blank control group and the sham operation group,the positive area of AQP5 in intervertebral discs in model control group was different(P<0.05),but there was no significant difference between the blank control group and the sham operation group(P>0.05),the rest had statistical significance(P<0.05).Conclusion:Qiangjinzhuangguqufeng mixture on the regulation of AQP1 and AQP5 is one of the therapeutic mechanisms,which can improve the degeneration of lumbar intervertebral disc.

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备注/Memo

备注/Memo:
基金项目:杭州市科技计划引导项目(2014KJJ01)
浙江省中医药科技计划A类项目(20152A157)
杭州市卫生科技计划重点项目(20150733Q60)
更新日期/Last Update: 2017-03-15