[1]上官文姬 李展春 程光齐 汤璐敏 李鹤△.骨碎补总黄酮对血管内皮细胞功能和去卵巢大鼠血管形成的影响[J].中国中医骨伤科杂志,2017,25(01):5-8,13.
　SHANGGUAN Wenji LI Zhanchun CHENG Guangqi TANG Lumin LI He.Effect of Drynaria Total Flavonoids on the Function of Vascular Endothelial Cells and Angiogenesis in Ovariectomy Rats[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2017,25(01):5-8,13.
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骨碎补总黄酮对血管内皮细胞功能和去卵巢大鼠血管形成的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第25卷
期数:
2017年01期
页码:
5-8,13
栏目:
实验研究
出版日期:
2017-01-15

文章信息/Info

Title:
Effect of Drynaria Total Flavonoids on the Function of Vascular Endothelial Cells and Angiogenesis in Ovariectomy Rats
文章编号:
1005-0205(2017)01-0005-04
作者:
上官文姬1 李展春2 程光齐2 汤璐敏1 李鹤1△
1上海交通大学医学院附属仁济医院中医科(上海,200127) 2上海交通大学医学院附属仁济医院骨科
Author(s):
SHANGGUAN Wenji1 LI Zhanchun2 CHENG Guangqi2 TANG Lumin1 LI He1△
1Department of Traditional Chinese Medicine,Renji Hospital Affiliated to Medical College of Shanghai Jiaotong University,Shanghai 200127,China; 2Department of Orthopedics,Renji Hospital Affiliated to Medical College of Shanghai Jiaotong University,Shanghai 200127,China.
关键词:
骨碎补总黄酮;  血管内皮细胞;  血管形成;  骨质疏松
Keywords:
drynaria total flavonoids;  vascular endothelial cell;  angiogenesis;  osteoporosis
分类号:
R-33
文献标志码:
A
摘要:
目的:分析骨碎补总黄酮对大鼠血管内皮细胞功能和血管形成的影响。方法:体外培养大鼠血管内皮细胞,用不同浓度(1,10,100 mg/L)的骨碎补总黄酮干预后,MTT法检测细胞增殖,ELISA法检测内皮素(ET)和血管内皮生长因子(VEGF),Griess法检测NO的表达; 利用去卵巢骨质疏松大鼠模型(36只,随机分为去卵巢组、假手术组和骨碎补治疗组),骨碎补总黄酮治疗3个月后,检测大鼠血清中ET,VEGF和NO的表达,骨密度和股骨远端微血管密度变化。结果:各浓度的骨碎补总黄酮均能促进血管内皮细胞的增殖,而中、高浓度促增殖效果较好; 中、高浓度的骨碎补总黄酮在体内外均可以抑制ET,同时增强NO的表达; 股骨远端血管计数结果显示骨碎补治疗组明显多于去卵巢组,并且与骨密度呈正相关; 骨碎补总黄酮在体外可以增强内皮细胞VEGF的表达,但对去卵巢大鼠血清中VEGF的表达无影响。结论:骨碎补总黄酮可促进血管内皮细胞增殖,同时可以调节内皮细胞功能,促进血管形成,从而发挥抗骨质疏松作用。
Abstract:
Objective:To investigate the effect of drynaria total flavonoids(DTF)on the function of vascular endothelial cells(VECs)and the angiogenesis in ovariectomy rats.Methods:The VECs of rats were cultured in vitro,and intervened with different concentrations(1,10,100 mg/L)of DTF.The proliferation of VECs was examined with MTT method,the levels of endothelin(ET)and VEGF were examined with ELISA method,and the expression of NO was detected with Griess method.The ovariectomized rat model of osteoporosis was used(36 rats were randomly divided into ovariectomized group,sham-operation group and drynaria treatment group).The expression of ET,VEGF and NO in the serum,the change of bone mineral density and micro-vessel density of the distal femur were measured 3 months after treating with DTF.Results:All the concentrations of DTF could promote the proliferation of VECs,while the effect of the medium and high concentration was better.The medium and high concentration of DTF could inhibit the expression of ET,while increase the expression of NO at the same time.It was showed that the osteoclast treatment group was significantly more than ovariectomized group in the vascular count of distal femur,and it was positively correlated with bone mineral density.DTF could enhance the expression of VEGF in endothelial cells,but was not effecting on the expression of VEGF in the serum of ovariectomized rats.Conclusion:DTF can promote the proliferation of VECs.Meanwhile,it can regulate the function of VECs,promote the angiogenesis,and thereby plays a role in anti-osteoporosis.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金-青年基金项目(81403443) 上海市卫计委中医药项目(2014JQ014A) 上海市‘杏林新星'资助计划 △通信作者 E-mail:lihe1972@hotmail.com
更新日期/Last Update: 2017-01-15