[1]郑志刚,张伦广△,潘三元,等.活血止痛汤联合脂肪填充对大鼠胫骨骨骺损伤的影响[J].中国中医骨伤科杂志,2024,32(03):12-16.[doi:10.20085/j.cnki.issn1005-0205.240303]
 ZHENG Zhigang,ZHANG Lunguang,PAN Sanyuan,et al.Effect of Huoxue Zhitong Decoction Combined with Fat Filling on the Injury of Fibial Epiphyseal in Fats[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2024,32(03):12-16.[doi:10.20085/j.cnki.issn1005-0205.240303]
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活血止痛汤联合脂肪填充对大鼠胫骨骨骺损伤的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第32卷
期数:
2024年03期
页码:
12-16
栏目:
实验研究
出版日期:
2024-03-15

文章信息/Info

Title:
Effect of Huoxue Zhitong Decoction Combined with Fat Filling on the Injury of Fibial Epiphyseal in Fats
文章编号:
1005-0205(2024)03-0012-05
作者:
郑志刚1张伦广1△潘三元1刘小钰1冯敏山2付亚君1张晓格1
1深圳市罗湖区中医院(广东 深圳,518001)
2中国中医科学院望京医院
Author(s):
ZHENG Zhigang1ZHANG Lunguang1△PAN Sanyuan1LIU Xiaoyu1FENG Minshan2FU Yajun1ZHANG Xiaoge1
1Luohu District Hospital of Traditional Chinese Medicine,Shenzhen 518001,Guangdong China; 2Wangjing Hospital,China Academy of Chinese Medical Sciences,Beijing 100102,China.
关键词:
大鼠胫骨远端Ⅲ型和Ⅳ型骨骺损伤 活血止痛汤 脂肪填充 骨桥 软骨细胞
Keywords:
type Ⅲ and Ⅳ epiphyseal injury of distal tibia in rats Huoxue Zhitong decoction fat filling bone bridge cartilage cells
分类号:
R-33
DOI:
10.20085/j.cnki.issn1005-0205.240303
文献标志码:
A
摘要:
目的:探讨活血止痛汤联合脂肪填充对大鼠胫骨远端Ⅲ型和Ⅳ型骨骺损伤的作用。方法:36只大鼠随机分为对照组、模型组、脂肪组和脂肪中药组,采用阿利新蓝、免疫荧光染色观察损伤模型的建立及骨骺损伤愈合过程、骨桥形成过程及血管内皮生长因子(VEGF)、骨形态发生蛋白2(BMP2)和白细胞介素1β(IL-1β)的表达情况。结果:阿利新蓝染色结果显示:相较于对照组,模型组骨骺损伤明显并形成骨桥,然而加入脂肪和活血止痛汤干预后,骨骺损伤明显改善,骨桥消失,脂肪中药组效果更为明显。免疫荧光结果表明:对比对照组,模型组VEGF、BMP2和IL-1β的表达均有明显增加; 而相较于模型组,脂肪组和脂肪中药组的VEGF、BMP2和IL-1β的表达均有所降低,并且脂肪中药组的VEGF、BMP2和IL-1β的表达下降更为明显。结论:活血止痛汤联合脂肪填充治疗大鼠胫骨远端Ⅲ型和Ⅳ型骨骺损伤疗效明显好于单纯脂肪干预。
Abstract:
Objective:To investigate the effect of Huoxue Zhitong decoction combined with fat filling on the type Ⅲ and Ⅳ epiphyseal injury of distal tibia in rats.Methods:36 rats were randomly divided into control group,model group,adipose group and adipose Chinese medicine group.The establishment of injury model,the healing process of epiphyseal injury,bone bridge formation and the expression of vascular endothelial growth factor(VEGF),bone morphogenetic protein-2(BMP2)and interleukin-1β(IL-1β)were observed by alcin blue staining and immunofluorescence staining.Results:Compared with the control group,alcin blue staining showed that the epiphyseal injury was obvious in the model group,and bone bridge was formed.However,after the intervention of adipose and Huoxue Zhitong decoction,the epiphyseal injury was obviously improved and bone bridge disappeared,and the effect of adipose Chinese medicine group was more obvious.Immunofluorescence results indicated that compared with the control group,the expressions of VEGF,BMP2 and IL-1β were significantly increased in the model group,while the expressions of VEGF,BMP2 and IL-1β were decreased in the fat group and the fat Chinese medicine group,and the expressions of VEGF,BMP2 and IL-1β were more significantly decreased in the fat Chinese medicine group.Conclusion:The therapeutic efficacy of Huoxue Zhitong decoction combined with fat filling on the type Ⅲ and Ⅳ epiphyseal injury of distal tibia in rats is significantly better than simple fat intervention.

参考文献/References:

[1] 李欢,沈忆新.胫骨远端骨折的治疗进展[J].生物骨科材料与临床研究,2007,4(4):30-32.
[2] 马长生,李郑林,王明千,等.小儿胫骨远端不稳定性骨折的治疗体会[J].中医正骨,2007,19(5):30.
[3] NGUYEN J C,MARKHARDT B K,MERROW A C,et al.Imaging of pediatric growth plate disturbances[J].Radiographics,2017,37(6):1791-1812.
[4] 韩乐乐.活血止痛汤加减在胫骨远端骨折术后肢体肿胀患者中的应用观察[J].实用中西医结合临床,2021,21(24):74-75.
[5] 时坤,孟凡军,冯哲.活血止痛汤对胫骨平台骨折患者术后DVT形成、凝血指标及关节功能的影响[J].分子诊断与治疗杂志,2023,15(6):990-993.
[6] 吴笛,戴丽冰,杨健生,等.活血止痛汤对胫骨平台骨折术后患者疼痛程度及膝关节功能的影响[J].中国民间疗法,2022,30(10):56-59.
[7] WATTENBARGER J M,GRUBER H E,PHIEFFER L S.Physeal fractures,part I:histologic features of bone,cartilage,and bar formation in a small animal model[J].J Pediatr Orthop,2002,22(6):703-709.
[8] LEE M A,NISSEN T P,OTSUKA N Y.Utilization of a murine model to investigate the molecular process of transphyseal bone formation[J].J Pedeatr Orthop,2000,20(6):802-806.
[9] 张克勇,余国荣,余黎,等.切除骨桥并填塞带血供脂肪筋膜瓣治疗中心型骨桥形成的研究[J].中华实验外科杂志,2013,30(11):2364-2366.
[10] LEE S U,LEE J Y,JOO S Y,et al.Transplantation of a scaffold-free cartilage tissue analogue for the treatment of physeal cartilage injury of the proximal tibia in rabbits[J].Yonsei Med J,2016,57(2):441-448.
[11] 邹永华,邱敏.活血止痛汤联合脱水剂治疗胫骨平台骨折术后患肢肿胀的临床疗效分析[J].中国医药指南,2023,21(15):49-52.
[12] FERRARA N,DAVIS-SMYTH T.The biology of vascular endothelial growth factor[J].Endocr Rev,1997,18(1):4-25.
[13] GERBER H P,VU T H,RYAN A M,et al.VEGF couples hypertrophic cartilage remodeling,ossification and angiogenesis during endochondral bone formation[J].Nature Medcine,1999,5(6):623-628.
[14] ZELZER E,MAMLUK R,FERRARA N,et al.VEGFA is necessary for chondrocyte survival during bone development[J].Development,2004,131(9):2161-2171.
[15] CHUNG R,XIAN C J.Recent research on the growth plate:mechanisms for growth plate injury repair and po-tential cell-based therapies for regeneration[J].J Mol Endocrinol,2014,53(1):T45-T61.
[16] FISCHERAUER E,HEIDARI N,NEUMAYER B,et al.Thespatial and temporal expression of VEGF and its receptors 1 and 2 in post-traumatic bone bridge formation of the growth plate[J].J Mol Histol,2011,42(6):513-522.
[17] SU Y W,CHUNG R,RUAN C S,et al.Neurotrophin-3 induces BMP-2 and VEGF activities and promotes the bony repair of injured growth plate cartilage and bone in rats[J].J Bone Miner Res,2016,31(6):1258-1274.
[18] SUN J,LI J,LI C,et al.Role of bone morphogenetic protein-2 in osteogenic differentiation of mesenchymal stem cells[J].Mol Med Rep,2015,12(3):4230-4237.
[19] FASSBENDER M,MINKWITZ S,THIELE M,et al.Efficacy of two different demineralised bone matrix grafts to promote bone healing in a critical-size-defect:a radiological,histological and histomorphometric study in rat femurs[J].Int Orthop,2014,38(9):1963-1969.
[20] SHU B,ZHANG M,XIE R,et al.BMP2,but not BMP4,is crucial for chondrocyte proliferation and maturation during endochondral bone development[J].J Cell Sci,2011,124(Pt 20):3428-3440.
[21] MOGHADDAM A,BREIER L,HAUBRUCK P,et al.Non-unions treated with bone morphogenic protein 7:introducing the quantitative measurement of human serum cytokine levels as promising tool in evaluation of adjunct non-union therapy[J].J Inflamm(Lond),2016,13(13):3.
[22] GERAGHTY S,KUANG JQ,YOO D,et al.A novel,cryopreserved,viable osteochondral allograft designed to augment marrow stimulation for articular cartilage repair[J].J Orthop Surg Res,2015,10:66.
[23] LIN D,ZUO S,LI L,et al.Treatment of neglected femoral neck fractures using the modified dynamic hip screw with autogenous bone and bone morphogenetic protein-2 composite materials grafting[J].Indian J Orthop,2015,49(3):342-346.
[24] MACSAI C E,GEORGIOU K R,FOSTER B K,et al.Microar ray expression analysis of genes and pathways involved in growth plate cartilage injury responses and bony repair[J].Bone,2012,50(5):1081-1091.
[25] DE CROMBRUGGHE B,LEFEBVRE V,NAKASHIMA K.Regulatory mechanisms in the pathways of cartilage and bone formation[J].Curr Opin Cell Biol,2001,13(6):721-727.
[26] YI H,ZHANG W,CUI Z M,et al.Resveratrol alleviates the interleukin-1β-induced chondrocytes injury through the NF-κB signaling pathway[J].J Orthop Surg Res,2020,15(1):424.
[27] JIANG J,CAI M.Cardamonin inhibited IL-1βinduced injury by inhibition of NLRP3 inflammasome via activating Nrf2/NQO-1 signaling pathway in chondroc[J].J Microbiol Biotechnol,2021,31(6):794-802.
[28] LU X,YU Y,YIN F,et al.Knockdown of PVT1 inhibits IL-1β-induced injury in chondrocytes by regulating miR-27b-3p/TRAF3 axis[J].International Immuno Pharmacology,2020,79:106052.

备注/Memo

备注/Memo:
基金项目:广东省中医药局中医药科研项目(20221346)
深圳市“医疗卫生三名工程”项目(SZZYSM202101005)
通信作者 E-mail:5098290@qq.com
更新日期/Last Update: 2024-03-15