[1]甄朋超△,阮安民,周乔,等.跌打万应膏治疗踝关节软组织损伤的实验研究[J].中国中医骨伤科杂志,2023,31(08):1-6+12.[doi:10.20085/j.cnki.issn1005-0205.230801 ]
　ZHEN Pengchao,RUAN Anmin,ZHOU Qiao,et al.Experimental Study of Diedawanying Ointment on the Treatment of Ankle Joint Soft Tissue Injury[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2023,31(08):1-6+12.[doi:10.20085/j.cnki.issn1005-0205.230801 ]
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跌打万应膏治疗踝关节软组织损伤的实验研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第31卷
期数:
2023年08期
页码:
1-6+12
栏目:
实验研究
出版日期:
2023-08-15

文章信息/Info

Title:
Experimental Study of Diedawanying Ointment on the Treatment of Ankle Joint Soft Tissue Injury
文章编号:
1005-0205(2023)08-0001-06
作者:
甄朋超1△; 阮安民2; 周乔1; 王磊1; 赵环宇1; 刘钢1
1北京中医药大学附属护国寺中医医院(北京,100035)
2北京市隆福医院
Author(s):
ZHEN Pengchao1△; RUAN Anmin2; ZHOU Qiao1; WANG Lei1; ZHAO Huanyu1; LIU Gang1
1Huguo Temple Chinese Medicine Hospital Affiliated to Beijing University of Chinese Medicine,Beijing 100035,China; 2Beijing Longfu Hospital,Beijing 100005,China.
关键词:
跌打万应膏;  踝关节;  软组织损伤;  机制
Keywords:
Diedawanying ointment;  ankle joint;  soft tissue injury;  mechanism
分类号:
R685
DOI:
10.20085/j.cnki.issn1005-0205.230801
文献标志码:
A
摘要:
目的:探讨跌打万应膏治疗踝关节软组织损伤的作用机制。方法:选取雄性大鼠72只,称重,随机分为6组,每组12只,分别为空白组、模型对照组、跌打万应膏高、中、低剂量组、对照组(冰敷),空白组不用药,其他组按体重各自给药。釆用经典的重锤坠落法造模建立大鼠踝关节急性软组织损伤模型,观察各组大鼠第3天和第7天后血清炎性递质IL-1β、TNF-α的水平以及应用荧光聚合酶链式反应(PCR)及蛋白质免疫印迹技术(Western Blot)检测各组大鼠受损软组织TLRs/MyD88/NF-κB通路表达水平的变化。结果:跌打万应膏可降低外周血中的IL-1β、TNF-α炎症因子水平,并显著抑制TLRs/MyD88/NF-κB信号通路TLR2、TLR4、MyD88、NF-κB基因及蛋白的激活和表达。结论:跌打万应膏外敷以中剂量疗效为佳,TLRs/MyD88/NF-κB信号通路可能是跌打万应膏的作用途径之一。
Abstract:
Objective:To investigate the mechanism of diedawanying ointment on the treatment of ankle soft tissue injury.Methods:72 male rats were selected and weighed and randomly divided into 6 groups,with 12 rats in each group,which were blank group,model control group,diedawanying ointment high-dose,medium-dose and low-dose groups,and control group(ice compress).Blank group was not given medicine,and other groups were given medicine according to body weight.Acute soft tissue injury model of the ankle joint in rats was established by the classical hammer fall method.The levels of serum inflammatory transmitters IL-1β and TNF-α were observed after 3 d and 7 d in each group,and the expression levels of TLRs/MyD88/NF-κB pathway in the damaged soft tissues of rats were detected by PCR and Western Blot.Results:Diedawanying ointment could decrease the levels of IL-1β and TNF-α inflammatory factors in peripheral blood,and significantly inhibit the activation and expression of TLR2,TLR4,MyD88 and NF-κB genes and proteins in TLRs/MyD88/NF-κB signaling pathway.Conclusion:Medium dose of diedawanying ointment has the best therapeutic effect.The action mechanism of diedawanying ointment is complex,and TLRs/MyD88/NF-κB signaling pathway is one of the action pathways of diedawanying ointment.

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备注/Memo

备注/Memo:
基金项目:首都卫生发展科研专项(首发2022-3-7029)
北京市第六批市级名老中医学术经验继承项目
△通信作者 E-mail:pengchaozhen@163.com
更新日期/Last Update: 2023-08-01