[1]梁国辉,周英杰△,谢艳,等.脊髓损伤大鼠体内存在的非编码核糖核酸调控神经递质的调控途径[J].中国中医骨伤科杂志,2023,31(07):6-11.[doi:10.20085/j.cnki.issn1005-0205.230702 ]
　LIANG Guohui,ZHOU Yingjie,XIE Yan,et al.A Regulatory Pathway of Non-Coding Ribonucleic Acid Regulating Neurotransmitters in Spinal Cord Injury Rats[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2023,31(07):6-11.[doi:10.20085/j.cnki.issn1005-0205.230702 ]
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脊髓损伤大鼠体内存在的非编码核糖核酸调控神经递质的调控途径()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第31卷
期数:
2023年07期
页码:
6-11
栏目:
实验研究
出版日期:
2023-07-15

文章信息/Info

Title:
A Regulatory Pathway of Non-Coding Ribonucleic Acid Regulating Neurotransmitters in Spinal Cord Injury Rats
文章编号:
1005-0205(2023)07-0006-06
作者:
梁国辉1; 周英杰1△; 谢艳1; 柴旭斌1; 禚汉杰1
1河南省洛阳正骨医院(河南省骨科医院)(河南 洛阳,471002)
Author(s):
LIANG Guohui1; ZHOU Yingjie1△; XIE Yan1; CHAI Xubin1; ZHUO Hanjie1
1Henan Luoyang Orthopedic Hospital(Henan Orthopedic Hospital),Luoyang 471002,Henan China.
关键词:
脊髓损伤;  长链非编码核糖核酸H19;  微小核糖核酸185-5p;  色氨酸羟化酶1;  5-羟色胺;  调控途径
Keywords:
spinal cord injury;  lncRNA H19;  miR-185-5p;  Tph1;  5-HT;  regulatory pathway
分类号:
R-33
DOI:
10.20085/j.cnki.issn1005-0205.230702
文献标志码:
A
摘要:
目的:验证脊髓损伤(SCI)大鼠体内存在的一条与lncRNA H19/miR-185-5p/5-HT轴相关的调控途径。方法:将20只SD大鼠分为模型组与实验组,实验组采用T2节段脊髓半横断法制作脊髓损伤大鼠模型,BBB评分法确定造模成功后,取两组脊髓组织测定5-HT含量和lncRNA H19与miR-185-5p的表达,然后双荧光素酶报告基因实验证实lncRNA H19调控miR-185-5p的表达,而miR-185-5p调控5-HT的合成酶Tph1的表达。进而体外培养脊髓神经元细胞并进行免疫荧光鉴定,然后观察lncRNA H19和miR-185-5p对脊髓损伤大鼠神经元细胞Tph1和5-HT表达的影响。最后通过对模型组、实验组和lncRNA H19敲基因大鼠组的对比,确定模型组和IncRNA H19敲基因大鼠体内上调的miR-185-5p的含量和下调的Tph1的表达丰度。结果:脊髓损伤大鼠体内存在lncRNA H19/miR-185-5p/Tph1调控途径。结论:可以以lncRNA H19/miR-185-5p/Tph1通路中任何一个重要因子作为治疗靶点进行精准治疗或直接以调控lncRNA H19或miR-185-5p为治理手段,进行针对大鼠脊髓损伤的治疗。
Abstract:
Objective:To investigate a novel regulatory pathway involving lncRNA H19/miR-185-5p/5-HT axis in rats with spinal cord injury.Methods:Twenty SD rats were divided into two groups:the model group and the experimental group.Spinal cord injury(SCI)rat models were created in the experimental group using T2 segmental spinal cord semi-transection.After confirming the modeling results using the BBB score,the levels of 5-HT and the expression of lncRNA H19 and miR-185-5p were measured in the spinal cord tissues of both groups.Subsequently,a double luciferase reporter assay was performed to confirm the regulation of miR-185-5p expression by lncRNA H19 and the regulation of 5-HT synthase Tph1 expression by miR-185-5pestablished and.In vitro cultures of spinal cord neurons were then identified using immunofluorescence.The effects of lncRNA H19 and miR-185-5p on the expression of Tph1 and 5-HT in spinal cord neurons of rats with spinal cord injury were observed.Finally,by comparingthe model group,experimental group,and lncRNA H19 knockout rats,the levels of up-regulated miR-185-5p and down-regulated Tph1 expression were determined.Results:The lncRNA H19/miR-185-5p/Tph1 pathway was identified in rats with spinal cord injury.Conclusion:The components of the lncRNA H19/miR-185-5p/Tph1 pathway could serve as potential therapeutic targets for precision therapy in spinal cord injury.Directly regulating the expression of lncRNA H19 or miR-185-5p could be a potential therapeutic approach for spinal cord injury in rats.

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备注/Memo

备注/Memo:
基金项目:河南省中医药科学研究专项课题(2019ZY1036)
△通信作者 E-mail:xy17737203229@163.com
更新日期/Last Update: 2023-07-10