[1]王杰,李琰,石威,等.基于肠道菌群的骨碎补抗绝经后骨质疏松症的机制研究[J].中国中医骨伤科杂志,2022,30(12):7-14.
　WANG Jie,LI Yan,SHI Wei,et al.Mechanism Study on Rhizoma Drynariae against Postmenopausal Osteoporosis Based on Intestinal Flora[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2022,30(12):7-14.
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基于肠道菌群的骨碎补抗绝经后骨质疏松症的机制研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第30卷
期数:
2022年12期
页码:
7-14
栏目:
实验研究
出版日期:
2022-12-15

文章信息/Info

Title:
Mechanism Study on Rhizoma Drynariae against Postmenopausal Osteoporosis Based on Intestinal Flora
文章编号:
1005-0205(2022)12-0007-08
作者:
王杰1; 李琰2; 石威1; 金鑫2; 李磊2; 侯燕2; 陈小鹏2; 王志洲1; 余昕2; 姜红江1; 2△
1安徽中医药大学第一临床医学院(合肥,230031) 2山东省文登整骨医院
Author(s):
WANG Jie1; LI Yan2; SHI Wei1; JIN Xin2; LI Lei2; HOU Yan2; CHEN Xiaopeng2; WANG Zhizhou1; YU Xin2; JIANG Hongjiang1; 2△
1The First Clinical School of Medicine, Anhui University of Chinese Medicine,Hefei 230031,China; 2Shandong Wendeng Osteopathic Hospital, Weihai 264400, Shandong China.
关键词:
绝经后骨质疏松症;  肠道菌群;  骨碎补;  信号通路;  作用机制
Keywords:
postmenopausal osteoporosis;  intestinal flora;  rhizoma drynariae;  signaling pathway;  mechanism
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨骨碎补防治绝经后骨质疏松症(PMOP)的作用机制及其与肠道菌群的关系。方法:将18只SPF级雌性SD大鼠随机分为模型组(VOX组)、假手术组(SHAM组)和骨碎补组(OVXDF组)。以手术方式构建绝经后骨质疏松症模型,持续灌胃12周。腹主动脉取血后处死,留取血清标本、双侧股骨、肠道粪便进行骨转化标志物检测及骨微结构分析,骨组织中Wnt/β-catenin相关蛋白的表达水平测定及肠道菌群结构和多样性分析。结果:与OVX组相比,SHAM组Tb.N、Tb.Th、Tb.Sp、NTX-I、Wnt3a、β-catenin、RUNX2、Faith-pd指数、香农指数差异有统计学意义(P<0.01),PINP、Faith-pd指数、Chao1指数差异有统计学意义(P<0.05)。与OVX组相比,OVXDF组Tb.N、Tb.Th、Tb.Sp、PINP、NTX-I、Wnt3a、β-catenin、RUNX2、香农指数差异有统计学意义(P<0.01),Chao1指数差异有统计学意义(P<0.05),Faith-pd指数差异无统计学意义(P>0.05)。结论:骨碎补可能是通过增加肠道菌群的丰度及多样性,调节肠道菌群结构并介导Wnt/β-catenin信号通路,来减少绝经后骨质疏松症模型大鼠的骨量丢失。
Abstract:
Objective:To explore the mechanism of rhizoma drynariae on the prevention and treatment of postmenopausal osteoporosis(PMOP)and its relationship with intestinal flora.Methods:18 SPF female SD rats were randomly divided into model group(OVX group), sham operation group(SHAM group)and rhizoma drynariae group(OVXDF group).The PMOP model was constructed surgically and continued to be given by gavage for 12 weeks.Blood sample was got from abdominal aorta before SD rats were sacrificed.Serum samples, bilateral femurs and intestinal feces were collected for bone transformation marker detection, bone microstructure analysis, the expression levels of Wnt/β-catenin-related proteins in bone tissue, and gut microbiota structure and diversity were analysed.Results:Compared with the OVX group, the SHAM group had significant differences in Tb.N, Tb.Th, Tb.Sp, NTX-I, Wnt3a, β-catenin, RUNX2 and Shannon index(P<0.01), and PINP, Chao1 index and Faith-pd index was statistically different(P<0.05).Compared with the OVX group, the Tb.N, Tb.Th, Tb.Sp, PINP, NTX-I, Wnt3a, β-catenin, RUNX2 and Shannon index of the OVXDF group were significantly different(P<0.01), Chao1 index was statistically different(P<0.05), Faith-pd index was not statistically different(P>0.05).Conclusion:Rhizoma drynaria may reduce bone loss in PMOP model rats by increasing the abundance and diversity of intestinal flora, regulating the structure of intestinal flora and mediating Wnt/β-catenin signaling pathway.

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备注/Memo

备注/Memo:
基金项目:山东省中医药科技项目(Q-2022030) 山东省中医药科技发展计划项目(2019-0798) △通信作者 E-mail:boneman@163.com
更新日期/Last Update: 2022-12-10