[1]侯费祎,李慎松,张莲,等.低氧环境下麝香含药血清对大鼠骨髓间充质干细胞成骨分化及血管生长因子表达的影响[J].中国中医骨伤科杂志,2022,30(12):1-6+14.
　HOU Feiyi,LI Shensong,ZHANG Lian,et al.Efficacy of Musk Medicated Serum on Osteogenic Differentiation and Secretion of Vascular Growth Factor of Rat Bone Marrow Mesenchymal Stem Cells under Hypoxia[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2022,30(12):1-6+14.
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低氧环境下麝香含药血清对大鼠骨髓间充质干细胞成骨分化及血管生长因子表达的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第30卷
期数:
2022年12期
页码:
1-6+14
栏目:
实验研究
出版日期:
2022-12-15

文章信息/Info

Title:
Efficacy of Musk Medicated Serum on Osteogenic Differentiation and Secretion of Vascular Growth Factor of Rat Bone Marrow Mesenchymal Stem Cells under Hypoxia
文章编号:
1005-0205(2022)12-0001-06
作者:
侯费祎1; 李慎松1; 张莲1; 邵宏斌1; 周燕1△
1中国人民解放军联勤保障部队第九四〇医院(兰州,730050)
Author(s):
HOU Feiyi1; LI Shensong1; ZHANG Lian1; SHAO Hongbin1; ZHOU Yan1△
1The 940 Hospital of the Joint Support Force of the Chinese People's Liberation Army, Lanzhou 730050,China.
关键词:
骨髓间充质干细胞;  低氧环境;  麝香;  血管生长因子
Keywords:
bone marrow mesenchymal stem cells;  osteogenic differentiation;  musk;  vascular growth factor
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨低氧环境下麝香含药血清对大鼠骨髓间充质干细胞成骨分化及血管生长因子表达的影响。方法:贴壁筛选提取大鼠骨髓间充质干细胞,提取P3代细胞,进行细胞形态学、表型及多向分化实验鉴定。低氧(3%)环境下培养骨髓间充质干细胞,利用O92细胞缺氧试剂盒检测不同时间点荧光强度,荧光越强说明细胞缺氧越明显; 分别制备麝香含药血清和空白对照组血清,并干预低氧环境骨髓间充质干细胞,检测其在低氧环境下对骨髓间充质干细胞成骨分化相关指标(碱性磷酸酶、骨钙素、钙盐)的影响及对血管生长因子(血管内皮生长因子(VEGF)、血管生成素(Angiogenin)、转化生长因子-β(TGF-β)、白细胞介素6(IL-6))表达的影响。结果:随着低氧培养时间延长,细胞缺氧逐渐加重,表现为O92标记的细胞荧光强度更强; 麝香含药血清组碱性磷酸酶、骨钙素、钙盐沉积量明显优于空白对照组,差异有统计学意义(P<0.05); 麝香含药血清组血管生长因子VEGF、Angiogenin、TGF-β表达明显优于空白对照组,差异有统计学意义(P<0.05),而IL-6的表达两组差异无统计学意义(P>0.05)。结论:麝香含药血清在低氧环境下对骨髓间充质干细胞成骨分化有促进作用,对骨髓间充质干细胞分泌的血管生长因子产生不同影响,能够促进骨髓间充质干细胞分泌VEGF、Angiogenin、TGF-β,而对IL-6的表达无明显影响。
Abstract:
Objective:To investigate the efficacy of musk medicated serum on osteogenic differentiation and secretion of vascular growth factor of rat bone marrow mesenchymal stem cells under hypoxia.Methods:Rat bone marrow mesenchymal stem cells were extracted by adherent screening, and P3 generation cells were extracted for identification of cell morphology, phenotype and multi-directional differentiation. Bone marrow mesenchymal stem cells were cultured under hypoxia(3%). The fluorescence intensity at different time points was detected by O92 cell hypoxia kit. The cell hypoxia was more obvious when the fluorescence intensity was stronger. The serum containing musk and the serum of the blank control group were prepared, and the bone marrow mesenchymal stem cells under hypoxia were intervened. The efficacy of the serum containing musk on the osteogenic differentiation of bone marrow mesenchymal stem cells(alkaline phosphatase, osteocalcin, calcium salt)and the expression of vascular growth factors(vascular endothelial growth factor(VEGF),Angiogenin,transforming growth factor-β(TGF-β),interleukin-6(IL-6)under hypoxia were detected.Results:With the prolongation of hypoxia culture time, cell hypoxia gradually increased, and the fluorescence intensity of O92 labeled cells was stronger. The deposition of alkaline lyase, osteocalcin and calcium salt in the serum containing musk was significantly better than that in the blank control group(P<0.05), with statistical difference. The expression of VEGF, Angiogenin and TGF-β in musk medicated serum group was significantly better than that in blank control group(P<0.05), with statistical difference, while the expression of IL-6 between the two groups was not statistically significant.Conclusion:The drug-containing serum of musk has a promoting efficacy on osteogenic differentiation of bone marrow mesenchymal stem cells in hypoxia environment, and has different efficacy on vascular growth factors secreted by bone marrow mesenchymal stem cells. It can promote the secretion of VEGF, Angiogenin and TGF-β by bone marrow mesenchymal stem cells, but has no significant efficacy on the expression of IL-6.

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备注/Memo

备注/Memo:
基金项目:甘肃省科技计划项目(20JR10RA009) 甘肃省卫生行业专项计划项目(GSWSKY2021-045) △通信作者 E-mail:houfeiyiqq@163.com
更新日期/Last Update: 2022-12-10