[1]樊孝俊△,何维佳,叶海程,等.人参皂苷Rg5对膝骨性关节炎模型大鼠软骨细胞和基质的影响[J].中国中医骨伤科杂志,2022,30(10):13-17+22.
　FAN Xiaojun,HE Weijia,YE Haicheng,et al.Efficacy of Ginsenoside-Rg5 on Chondrocytes and Cartilage Matrix in Rats with Knee Osteoarthritis Model[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2022,30(10):13-17+22.
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人参皂苷Rg5对膝骨性关节炎模型大鼠软骨细胞和基质的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第30卷
期数:
2022年10期
页码:
13-17+22
栏目:
实验研究
出版日期:
2022-10-15

文章信息/Info

Title:
Efficacy of Ginsenoside-Rg5 on Chondrocytes and Cartilage Matrix in Rats with Knee Osteoarthritis Model
文章编号:
1005-0205(2022)10-0013-05
作者:
樊孝俊1△; 何维佳1; 叶海程1; 苏春涛1
1厦门大学附属第一医院康复医学科(福建 厦门,361003)
Author(s):
FAN Xiaojun1△; HE Weijia1; YE Haicheng1; SU Chuntao1
1Department of Rehabilitation Medicine,First Affiliated Hospital of Xiamen University,Xiamen 361003,Fujian China.
关键词:
膝骨性关节炎;  人参皂苷Rg5;  软骨细胞;  软骨基质
Keywords:
knee osteoarthritis;  ginsenoside-Rg5;  chondrocytes;  cartilage matrix
分类号:
R684.3
文献标志码:
A
摘要:
目的:观察人参皂苷Rg5(G-Rg5)对膝骨性关节炎(KOA)大鼠模型的关节软骨细胞、糖胺聚糖(GAG)代谢的影响。方法:采用离断前交叉韧带造模24只膝骨性关节炎模型大鼠,分为空白对照组(NC组)、生理盐水组(NS组)及关节腔注射不同浓度人参皂苷的3 μg和10 μg G-Rg5组,每周注射2次,重复6周。组织形态学观察采用苏木精-伊红(HE)染色和番茄O-固绿染色,评估改良的Mankin评分(MMS)、软骨细胞计数、软骨厚度及软骨基质糖胺聚糖含量。结果:组织形态学染色可见NS组软骨表面粗糙甚至局部缺损,基质染色不均,软骨细胞簇集,软骨层次不清晰,潮线消失,呈明显骨性关节炎样改变。相比NS组,10 μg G-Rg5组软骨表面相对平整,基质染色均匀,潮线存在,软骨细胞数、软骨厚度和糖胺聚糖含量明显升高,MMS明显降低(P<0.05),甚至MMS和糖胺聚糖含量接近NC组(P>0.05)。而3 μg G-Rg5组软骨表面不平整,基质染色不均,呈轻度骨性关节炎样特点,全层软骨细胞数和软骨厚度、糖胺聚糖含量无明显升高(P>0.05)。结论:G-Rg5对膝骨性关节炎具有保护作用,可以改善或延缓关节软骨损伤,且呈剂量依赖性抑制膝骨性关节炎模型大鼠的关节软骨细胞凋亡和基质糖胺聚糖含量减少。
Abstract:
Objective:To observe the efficacy of ginsenoside-Rg5(G-Rg5)on the metabolism of articular chondrocytes and glycosaminoglycans in rats with knee osteoarthritis(KOA).Methods:24 KOA model rats established by transecting anterior cruciate ligament were divided into the normal control group(NC),the normal saline group(NS),the 3 μg G-Rg5 group and 10 μg G-Rg5 group.All injection was carried out twice a week for 6 weeks.The cartilage morphology was evaluated by hematoxylin-eosin(HE)staining and Safranin O-Fast Green staining.Modified Mankin's scores(MMS),cartilage glycosaminoglycans(GAG)content,number of chondrocyte and cartilage thickness were evaluated.Results:Histological staining showed that the NS group had rough surface or even local defects,uneven coloration cartilage matrix,chondrocyte clustering,unclear cartilage layers,tidemark disappearance and presenting obvious characteristics of osteoarthritis-like changes.Compared with NS group,10 μg G-Rg5 group had a relatively smooth cartilage surface,uniform coloration cartilage matrix,present tidemark line and signific improvement of the number of chondrocytes,cartilage thickness and GAG content,and its MMS was significantly decreased(P<0.05).10 μg G-Rg5 group in cartilage MMS and GAG content closed to NC group(P>0.05).However,the 3 μg G-Rg5 group had uneven cartilage surface and uneven coloration cartilage matrix,mild osteoarthritis-like characteristics,and the number of full-layer chondrocytes,cartilage thickness and GAG content in 3 μg G-Rg5 group were not significantly increased(P>0.05).Conclusion:G-Rg5 had a protective efficacy on KOA and can improve or delay articular cartilage injury,and inhibit chondrocyte apoptosis and lose of GAG content of KOA model rats in a dose-dependent manner.

参考文献/References:

[1] ROOS E M,ARDEN N K.Strategies for the prevention of knee osteoarthritis[J].Nat Rev Rheumatol,2016,12(2):92-101.
[2] KWON S W,HAN S B,PARK I H,et al.Liquid chromatographic determination of less polar ginsenosides in processed ginseng[J].J Chromatogr A,2001,921(2):335-339.
[3] KIM S K,PARK J H.Trends in ginseng research in 2010[J].J Ginseng Res,2011,35(4):389-398.
[4] KIM S N,HA Y W,SHIN H,et al.Simultaneous quantification of 14 ginsenosides in Panax ginseng C.A.Meyer(Korean red ginseng)by HPLC-ELSD and its application to quality control[J].J Pharm Biomed Anal,2007,45(1):164-170.
[5] KIM T W,JOH E H,KIM B,et al.Ginsenoside Rg5 ameliorates lung inflammation in mice by inhibiting the binding of LPS to toll-like receptor-4 on macrophages[J].Int Immunopharmacol,2012,12(1):110-116.
[6] LIU M Y,LIU F,LI Y J,et al.Ginsenoside Rg5 inhibits human osteosarcoma cell proliferation and induces cell apoptosis through PI3K/Akt/mTOR C1-related LC3 autophagy pathway[J].Oxid Med Cell Longev,2021:5040326.
[7] DONG Y,FU R,YANG J,et al.Folic acid-modified ginsenoside Rg5-loaded bovine serum albumin nanoparticles for targeted cancer therapy in vitro and in vivo[J].Int J Nanomedicine,2019,14:6971-6988.
[8] ZHANG P.Ginsenoside-Rg5 treatment inhibits apoptosis of chondrocytes and degradation of cartilage matrix in a rat model of osteoarthritis[J].Oncol Rep,2017,37(3):1497-1502.
[9] ROGART J N,BARRACH H J,CHICHESTER C O.Articular collagen degradation in the Hulth-Telhag model of osteoarthritis[J].Osteoarthritis Cartilage,1999,7(6):539-547.
[10] 李文雄,孙赫,沈玮,等.SD大鼠骨关节炎造模方法的筛选及优化探究[J].畜牧与兽医,2014,46(10):70-73.
[11] CHENG J H,CHOU W Y,WANG C J,et al.Pathological,morphometric and correlation analysis of the Modified mankin score,tidemark roughness and calcified cartilage thickness in rat knee osteoarthritis after extracorporeal shockwave therapy[J].Int J Med Sci,2022,19(2):242-256.
[12] ARAVINTHAN A,HOSSAIN M A,KIM B,et al.Ginsenoside Rb1 inhibits monoiodoacetate-induced osteoarthritis in postmenopausal rats through prevention of cartilage degradation[J].J Ginseng Res,2021,45(2):287-294.
[13] LIU M Y,LIU F,GAO Y L,et al.Pharmacological activities of ginsenoside Rg5(Review)[J].Exp Ther Med,2021,22(2):840.
[14] MEDZHITOV R.Origin and physiological roles of inflammation[J].Nature,2008,454(7203):428-435.
[15] LIU Y,FAN D.The preparation of Ginsenoside Rg5,its antitumor activity against breast cancer cells and its targeting of PI3K[J].Nutrients,2020,12(1):246.
[16] LIU Y,FAN D.Ginsenoside Rg5 induces G2/M phase arrest,apoptosis and autophagy via regulating ROS-mediated MAPK pathways against human gastric cancer[J].Biochem Pharmacol,2019,168:285-304.
[17] CUI Y,SU Y,DENG L,et al.Ginsenoside-Rg5 inhibits retinoblastoma proliferation and induces apoptosis through suppressing BCL2 expression[J].Chemotherapy,2018,63(5):293-300.
[18] 左显锋,范建楠,莫愁,等.骨性关节炎关节软骨细胞凋亡的研究进展[J].山东医药,2022,62(4):108-111.
[19] HASHIMOTO R,YU J,KOIZUMI H,et al.Ginsenoside Rb1 prevents MPP(+)-induced apoptosis in PC12 cells by stimulating estrogen receptors with consequent activation of ERK1/2,Akt and inhibition of SAPK/JNK,p38 MAPK[J].Evid Based Complement Alternat Med,2012:693717.
[20] RADAD K,GILLE G,MOLDZIO R,et al.Ginsenosides Rb1 and Rg1 effects on mesencephalic dopaminergic cells stressed with glutamate[J].Brain Res,2004,1021(1):41-53.
[21] 李威,杨凤云.鼠类膝骨性关节炎模型建立的研究进展[J].江西中医药,2020,51(2):77-80.
[22] INADA M,WANG Y,BYRNE M H,et al.Critical roles for collagenase-3(MMP13)in development of growth plate cartilage and in endochondral ossification[J].Proc Natl Acad Sci U S A,2004,101(49):17192-17197.
[23] KNÄUPER V,BAILEY L,WORLEY J R,et al.Cellular activation of proMMP-13 by MT1-MMP depends on the C-terminal domain of MMP-13[J].FEBS Lett,2002,532(1/2):127-130.
[24] HOSSAIN M A,ALAM M J,KIM B,et al.Ginsenoside-Rb1 prevents bone cartilage destruction through down-regulation of p-Akt,p-P38,and p-P65 signaling in rabbit[J].Phytomedicine,2022,100:154039.

备注/Memo

备注/Memo:
基金项目:福建中医药大学校管课题(XB2021088)
△通信作者 E-mail:fanxj@xmu.edu.cn
更新日期/Last Update: 2022-10-10