[1]谭开云,卢敏,邝高艳△.骨科大手术后下肢深静脉血栓大鼠模型的改良及评价[J].中国中医骨伤科杂志,2022,30(10):1-6.
　TAN Kaiyun,LU Min,KUANG Gaoyan.Modified Rat Model of Lower Extremity Deep Venous Thrombosis after Major Orthopaedic Surgery[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2022,30(10):1-6.
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骨科大手术后下肢深静脉血栓大鼠模型的改良及评价()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第30卷
期数:
2022年10期
页码:
1-6
栏目:
实验研究
出版日期:
2022-10-15

文章信息/Info

Title:
Modified Rat Model of Lower Extremity Deep Venous Thrombosis after Major Orthopaedic Surgery
文章编号:
1005-0205(2022)10-0001-06
作者:
谭开云1; 卢敏1; 邝高艳1△
1湖南中医药大学第一附属医院(长沙,410007)
Author(s):
TAN Kaiyun1; LU Min1; KUANG Gaoyan1△
1The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China.
关键词:
骨科大手术;  下肢深静脉血栓;  动物造模;  静脉结扎;  骨折创伤模型
Keywords:
major orthopaedic surgery;  lower extremity deep venous thrombosis;  animal modeling;  vein ligation;  fracture trauma model
分类号:
R-33
文献标志码:
A
摘要:
目的:通过构建一种改良的骨科大手术后下肢深静脉血栓形成模型,与下肢深静脉血栓传统模型对比,评价该造模方法的可靠性和安全性。方法:将30只大鼠随机分为3组,每组10只,其中A组采用骨折法造模,通过重物击打致股骨颈骨折,采取髋部人字石膏固定; B组采用骨折改良法造模,通过重物击打致股骨颈骨折,行大腿近端外侧入路切开复位骨折断端清理术,术后行髋部人字石膏固定; C组采用静脉结扎法造模,通过剑突下腹部正中切口行下腔静脉和两侧髂腰静脉结扎术。术后持续肉眼观察大鼠末端皮肤肿胀程度和皮肤颜色的变化,同时取大鼠大隐静脉1～2 cm经过石蜡包埋切片,苏木精-伊红(HE)染色,光镜下观察血栓形成情况以及血管壁的变化。结果:骨折改良法造模,死亡1只,成功6只; 双足外形肿胀,皮肤颜色偏紫; 肉眼观察3只大鼠存在1级血栓,3只大鼠存在2级血栓; 光镜下观察2只大鼠血管样本存在1级血栓,2只大鼠血管样本存在2级血栓,2只大鼠血管样本存在3级血栓。改良造模方法成功,安全性较好。结论:通过击打导致骨折,行切开复位手术后髋人字石膏固定的骨科大手术后下肢深静脉血栓的造模方法理论依据充分,造模成功的评定标准客观,操作较为简单,可重复性高,契合骨科临床实际,为骨科大手术后下肢深静脉血栓形成的临床研究和基础研究提供了一种新的造摸方法。
Abstract:
Objective:To explore the effectiveness and safety of a new a lower extremity deep vein thrombosis model after major orthopedic surgery, as compared with traditional methods.Methods:Thirty rats were randomly divided into 3 groups with 10 rats in each group. Group A was modeled by fracture method. The femoral neck was fractured by hitting with heavy objects, and the hip herringbone plaster was used. Group B was modeled by modified fracture method. The femoral neck was fractured by hitting with heavy objects. Open reduction and debridement of the fracture through the proximal lateral approach of the thigh were performed, and the hip herringbone plaster was performed after the operation. The vein ligation method was used to create the model in group C. The xiphoid process was used to create the model. The inferior vena cava and bilateral iliolumbar veins were ligated through a median incision in the lower abdomen. After operation, the degree of skin swelling and the changes of skin color at the end of the rats were continuously observed with the naked eye. At the same time 1 to 2 cm of the great saphenous vein of the rats was taken for paraffin-embedded sections, stained with hematoxylin-eosin(HE). The thrombosis and the changes of the blood vessel wall were observed under the light microscope.Results:1 rat died and 6 succeeded in modeling by improved fracture method. The feet were swollen, and the skin color was purplish. 3 rats had grade 1 thrombus and 3 rats had grade 2 thrombus under the microscope. There were grade 1 thrombus in rat blood vessel samples, grade 2 thrombus in two rat blood vessel samples, and grade 3 thrombus in two rat blood vessel samples. Compared with fracture modeling and vein ligation modeling, the improved modeling methodology was successful.Conclusion:The modeling method of lower extremity deep venous thrombosis after major orthopaedic surgery leads to fracture and hip herringbone plaster fixation after open reduction, which has sufficient theoretical basis, and the evaluation standard of successful modeling is objective, simple operation, high repeatability, which is suitable for orthopaedic clinic. It provides a new method for the clinical and basic research of lower extremity deep venous thrombosis after major orthopaedic surgery.

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备注/Memo

备注/Memo:
基金项目:中央补助项目——第六批名老中医药专家学术经验继承指导(rsk-011-13)
湖南中医药大学第一附属医院名院工程项目(rsk-010-14)
国家中医药管理局全国名老中医药专家传承工作室建设项目(国中医药人教函〔2022〕75号)
湖南省中医药管理局“十四五”第一批中医药领军人才培养项目湖南省教育厅课题(19C1400)
△通信作者 E-mail:kuangyi0109@126.com
更新日期/Last Update: 2022-10-10