[1]赵云超 李晓明 王恒俊 周婷婷 房庆鹏 赵阳.丹参酮2A治疗股骨头坏死的作用机制研究[J].中国中医骨伤科杂志,2022,30(07):1-5.
　ZHAO Yunchao LI Xiaoming WANG HengjunZHOU Tingting FANG Qingpeng ZHAO Yang.Study on the Mechanism of Tanshinone 2A on the Treatment of Femoral Head Necrosis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2022,30(07):1-5.
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丹参酮2A治疗股骨头坏死的作用机制研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第30卷
期数:
2022年07期
页码:
1-5
栏目:
实验研究
出版日期:
2022-07-15

文章信息/Info

Title:
Study on the Mechanism of Tanshinone 2A on the Treatment of Femoral Head Necrosis
文章编号:
1005-0205(2022)07-0001-05
作者:
赵云超1 李晓明1 王恒俊1 周婷婷1 房庆鹏1 赵阳1
1河北省沧州中西医结合医院(河北 沧州,061001)
Author(s):
ZHAO Yunchao1 LI Xiaoming1 WANG Hengjun1ZHOU Tingting1 FANG Qingpeng1 ZHAO Yang1
1Hebei Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine,Cangzhou 061001,Hebei China.
关键词:
丹参酮2A;  股骨头坏死;  磷脂酰肌醇3激酶-丝氨酸/苏氨酸激酶信号通路;  骨保护素;  骨钙素;  增殖;  凋亡
Keywords:
Tanshinone 2A;  necrosis of femoral head;  phosphatidylinositol 3 kinase-serine/threonine kinase signal pathway;  osteoprotegerin;  osteocalcin;  proliferation;  apoptosis
分类号:
R681
文献标志码:
A
摘要:
目的:探究丹参酮2A对股骨头坏死小鼠的作用机制及与磷脂酰肌醇3激酶(Phosphatidylinositol Kinase,PI3K)-丝氨酸/苏氨酸激酶(Threonine Kinase,Akt)信号通路之间的关系。方法:脂多糖联合甲基强的松龙构建股骨头坏死小鼠模型,丹参酮2A给药模型小鼠; 苏木精-伊红(HE)观察股骨头坏死、空骨陷窝; 蛋白免疫印迹(Western Blotting,WB)实验检测股骨头组织或细胞中骨保护素(Osteoprotegerin,OPG)、骨钙素(Osteocalcin,OCN)、含NLR家族PYRIN域蛋白3(Nucleotide-binding oligomerization domain,Leucine-rich Repeat and PYRIN Domain-containing 3,NLRP3)、p-PI3K、PI3K、p-Akt、Akt的蛋白表达; 地塞米松(Dex)处理hFOB1.19细胞,模拟股骨头细胞损伤,丹参酮2A给药处理; 细胞计数试剂盒(CCK8)实验、膜联蛋白V-异硫氰酸荧光素-碘化丙锭(Annexin V-fluorescein isothiocyanate/propidium iodide,Annexin V-FITC/PI)细胞凋亡检测实验检测细胞增殖率、凋亡率。结果:与对照组相比,模型组小鼠股骨头坏死率(χ2=12.929,P<0.05)、空骨陷窝率(t=8.027,P<0.05)均显著升高,OPG、OCN蛋白表达显著降低,NLRP3蛋白表达显著升高,p-PI3K、p-Akt蛋白表达显著降低,差异有统计学意义(P<0.05)。与模型组相比,观察组小鼠以上指标均发生反向变化,趋于对照组。与空白组相比,Dex组hFOB1.19细胞增殖率显著降低,凋亡率显著升高,OPG、OCN蛋白表达显著降低,NLRP3蛋白表达显著升高,p-PI3K、p-Akt蛋白表达显著降低,差异有统计学意义(P<0.05)。与Dex组相比,丹参酮2A组细胞上述指标均反向变化。结论:丹参酮2A可减轻股骨头坏死的损伤,其作用机制与PI3K/Akt信号通路的活性有关。
Abstract:
Objective:To investigate the efficacy and mechanism of Tanshinone 2A on mice with femoral head necrosis,and the relationship with phosphatidylinositol 3 kinase(PI3K)-serine/threonine kinase(Akt)signal pathway.Methods:Lipopolysaccharide combined with methylprednisolone was used to establish a femoral head necrosis mouse model,and Tanshinone 2A was administered to the mouse model.Hematoxylin eosin(HE)was used to observe the necrosis of femoral head and hollow bone.Western Blotting(WB)was used to detect osteoprotegerin(OPG),osteocalcin(OCN),PYRIN domain protein 3(nucleotide binding oligomerization domain,Leucine-rich repeat and PYRIN domain-containing 3,NLRP3),p-PI3K,PI3K,p-Akt,Akt protein expression.Dexamethasone(Dex)was used to treat hFOB1.19 cells to simulate the injury of femoral head cells,and Tanshinone 2A was used for treatment.Cell counting kit(CCK8)experiment and Annexin V-fluorescein isothiocyanate/propidium iodide(Annexin V-FITC/PI)apoptosis detection experiment were used to detect the cell proliferation rate and apoptosis rate.Results:Compared with the control group,the necrosis rate of femoral head(χ2=12.929)and empty bone lacuna rate(t=8.027)were significantly increased,the protein expression of OPG and OCN were significantly decreased,the protein expression of NLRP3 was significantly increased,and the protein expression of p-PI3K and p-Akt were significantly decreased(P<0.05)in the model group.Compared with the model group,the above indexes of micechanged inversely in the observation group and tended to the control group.Compared with the blank group,the proliferation rate,apoptosis rate,OPG and OCN protein expression,NLRP3 protein expression,p-PI3K and p-Akt protein expression were significantly decreased in Dex group(P<0.05).Compared with Dex group,the above indexes changed inversely in Tanshinone 2A group.Conclusion:Tanshinone 2A could reduce the injury of femoral head necrosis,and its mechanism may be related to the activity of PI3K/Akt signal pathway.

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备注/Memo

备注/Memo:
基金项目:河北省中医药管理局中医药类科研计划课题(2020468)
更新日期/Last Update: 1900-01-01