[1]韩雪 张军△ 韩磊 耿进朝 崔莹.慢加速与快加速旋转手法对香草素受体4/一氧化氮通路抑制强度的比较[J].中国中医骨伤科杂志,2021,29(12):22-25.
　HAN Xue ZHANG Jun HAN Lei GENG Jinchao CUI Ying.Observation on the Efficacy of TRPV4/NO Expression in DRG by Slowly-Accelerating or Fastly-Accelerating Types of Spinal Manipulation on Acute Radiculopathy in Rat Model[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2021,29(12):22-25.
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慢加速与快加速旋转手法对香草素受体4/一氧化氮通路抑制强度的比较()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第29卷
期数:
2021年12期
页码:
22-25
栏目:
实验研究
出版日期:
2021-12-15

文章信息/Info

Title:
Observation on the Efficacy of TRPV4/NO Expression in DRG by Slowly-Accelerating or Fastly-Accelerating Types of Spinal Manipulation on Acute Radiculopathy in Rat Model
文章编号:
1005-0205(2021)12-0022-04
作者:
韩雪1 张军1△ 韩磊1 耿进朝1 崔莹1
Author(s):
HAN Xue ZHANG Jun HAN Lei GENG Jinchao CUI Ying
1Wangjing Hospital of Chinese Academy of Traditional Chinese Medical Sciences,Beijing 100102,China.
关键词:
旋转手法;  加速度;  香草素受体4/一氧化氮;  疼痛
Keywords:
rotating manipulation;  acceleration;  transient receptor potential vanilloid 4/nitric oxide;  pain
分类号:
R-33
文献标志码:
A
摘要:
目的:比较慢加速与快加速旋转手法对大鼠腰椎背根节(DRG)损伤模型机械痛敏、TRPV4通道蛋白和亚硝酸盐的影响,探讨不同加速度载荷旋转手法的消炎祛痛效果。方法:192只雄性SD大鼠随机分为低速-零加速旋转手法组、低速-慢加速旋转手法组、低速-快加速旋转手法组、阳性药物灌胃对照组,每组各48只。所有大鼠均经L5～6椎间孔外口先插入钢棒“致压”、后植入髓核“致炎”的方法建立DRG损伤疼痛模型,分别施行模拟L5～6关节假手法、模拟L5～6关节松动手法、模拟L5～6关节调整手法、COX-2抑制药Etoricoxib内服。每组各取6只大鼠于术前1 d及术后第1,7,15,21天行机械性撤足痛阈值(PWT)的测定; 每组各取24只大鼠于术后第9天和第21天时取出手术同侧的L5 DRG,其中18只用于TRPV4通道蛋白表达的检测; 6只用于NO代谢产物亚硝酸盐含量的检测。结果:低速-快加速组50%PWT 在术后第7天时开始高于低速-零加速组,差异有统计学意义(P<0.05); 第15天和第21天时更高,差异有统计学意义(P<0.01)。低速-慢加速组50%PWT在术后第1天时高于低速-快加速组,差异有统计学意义(P<0.05); 但第7,15,21 天时两者并无差别,差异无统计学意义(P>0.05)。低速-慢加速组与Etoricoxib组相比较并无差别,差异无统计学意义(P>0.05)。经腰椎旋转手法干预后,SNK检验显示在术后第9天和第21天时低速-慢加速组、低速-快加速组、Etoricoxib组的TRPV4蛋白平均灰度值、亚硝酸盐含量两两比较并无差异,但均明显低于低速-零加速组(P<0.01)。结论:慢加速与快加速模拟手法对TRPV4/NO通路的抑制强度无差异,均与口服Etoricoxib的效果相当。在其他力学影响因素不变的情况下,不同的加速度载荷并不影响最终的消炎祛痛效果。
Abstract:
Objective:To compare the efficacies of fastly-accelerating and slowly-accelerating types of spinal manipulation on mechanical hyperalgesia,TRPV4 channel protein expression and nitrite content in rat dorsal root ganglion(DRG)injury model with acute radiculopathy and to explore the anti-inflammatory and analgesic efficacies of rotating manipulation under different acceleration loads.Methods:192 male SD rats were randomly divided into low-speed and no-accelerating manipulation group,low-speed and fastly-accelerating manipulation group,low-speed and slowly-accelerating manipulation group and positive medicine gavage control group,with 48 rats in each group.In all rats,steel rods were inserted into the outer orifice of L5～6 intervertebral foramen to induce pressure,then nucleus pulposus were applied to induce inflammation,and finally DRG injury models were established.Four groups were performed Simulated L5～6 joint sham manipulation,simulated L5～6 joint loosening manipulation,simulated L5～6 joint adjustment manipulation and oral administration of Etoricoxib,a COX-2 inhibitor.Six rats in each group were selected to measure the 50% mechanical paw withdrawal threshold(PWT)one day before modeling and 1,7,15,21 d after modeling.The left L5 DRG of 24 rats in each group were collected on days 9,21 respectively after surgery to detect the expression of TRPV4 with 18 rats and nitrite content with 6 rats in each group.Results:The 50%PWT in the low-speed and fastly-accelerating group was higher than that in the low-speed and no-accelerating manipulation group on the 7th day after operation(P<0.05)and was higher at the 15th and 21st day(P<0.01).The 50% PWT in the low-speed and slowly-accelerating manipulation group was higher than that in the low-speed and fastly-accelerating group on the 1st day after operation(P<0.05),but there was no difference between the two groups on the 7th,15th and 21st days(P>0.05).There was no difference between the low-speed and slowly-accelerating manipulation group and positive drug gavaged control group(P>0.05).SNK test showed that there was no difference in the TRPV4 protein gray value and nitrite content among low-speed and fastly-accelerating manipulation group,low-speed and slowly-accelerating manipulation group and positive drug gavaged control group at 9 and 21 d after operation,but they were significantly lower than those in low-speed and no-accelerating manipulation group(P<0.01).Conclusion:There is no difference in the inhibition intensity of TRPV4/NO pathway between low-speed and slowly-accelerating and low-speed and fastly-accelerating manipulation,which are similar to Etoricoxib gavage.In the case of other mechanical factors unchanged,different accelerating load does not affect the final anti-inflammatory and analgesic efficacy.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金面上项目(81373657)国家自然科学基金青年项目(81503602)1中国中医科学院望京医院(北京,100102)△通信作者 E-mail:zhangjunmd@163.com
更新日期/Last Update: 1900-01-01