[1]彭莎 姚楠 卢岩岩 许学猛△ 吴淮 刘文刚 黄雪君 陈国材.补肾强筋胶囊对膝骨关节炎的治疗作用及分子机制研究[J].中国中医骨伤科杂志,2021,29(04):13-19.
　PENG Sha YAO Nan LU Yanyan XU Xuemeng WU HuaiLIU Wengang HUANG Xuejun CHEN Guocai.Study on the Effect and Molecular Mechanism of BushenqiangjinCapsule on Knee Osteoarthritis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2021,29(04):13-19.
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补肾强筋胶囊对膝骨关节炎的治疗作用及分子机制研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第29卷
期数:
2021年04期
页码:
13-19
栏目:
实验研究
出版日期:
2021-04-15

文章信息/Info

Title:
Study on the Effect and Molecular Mechanism of BushenqiangjinCapsule on Knee Osteoarthritis
文章编号:
1005-0205(2021)04-0013-07
作者:
彭莎1; 2; 3 姚楠1; 2; 3 卢岩岩1 许学猛1; 2△ 吴淮1; 2 刘文刚1; 2 黄雪君1; 2; 3 陈国材1
1广州中医药大学第五临床医学院(广州,510095)
2广东省第二中医院(广东省中医药工程技术研究院)
3广东省中医药研究开发重点实验室
Author(s):
PENG Sha1; 2; 3 YAO Nan1; 2; 3 LU Yanyan1 XU Xuemeng1; 2△ WU Huai1; 2LIU Wengang1; 2 HUANG Xuejun1; 2; 3 CHEN Guocai1
1The Fifth Clinical College of Guangzhou University of Chinese Medicine,Guangzhou 510095,China; 2Guangdong Second Traditional Chinese Medicine Hospital(Guangdong Province Engineering Technology Research Institute of Traditional Chinese Medicine),Guangzhou 510095,China; 3Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine,Guangzhou 510095,China.
关键词:
补肾强筋胶囊;  膝骨关节炎;  自噬;  分子机制
Keywords:
Bushenqiangjin capsule;  knee osteoarthritis;  autophagy;  molecular mechanism
分类号:
R684.3
文献标志码:
A
摘要:
目的:探讨补肾强筋胶囊对膝骨关节炎(KOA)的治疗作用及相关分子机制。方法:将40只SD大鼠随机分为4组,每组10只。采用关节腔注射木瓜蛋白酶和半胱氨酸建立KOA大鼠模型。正常组和模型组给予生理盐水灌胃,补肾高、低剂量组分别灌胃0.486 g/kg和0.243 g/kg补肾强筋胶囊。6周后处死大鼠并采集膝关节软骨和血清进行检测。苏木精-伊红(HE)染色和番红-固绿染色观察各组大鼠膝关节软骨病理变化并进行改良Mankin评分; 酶联免疫吸附测定(ELISA)检测大鼠血清白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)水平; 实时荧光定量PCR和蛋白免疫印迹(Western Blot)检测MMP13、Beclin1、LC3B、ULK1、p-AMPK、p-mTOR mRNA和蛋白的表达。结果:与模型组相比,补肾高、低剂量组大鼠膝关节软骨退变程度明显减轻且Mankin评分显著降低,差异有统计学意义(P<0.01)。补肾高、低剂量组大鼠血清IL-1β和TNF-α水平显著降低,差异有统计学意义(P<0.01)。补肾高、低剂量组大鼠软骨MMP13 mRNA和蛋白表达显著降低,Beclin1、LC3B-Ⅱ/Ⅰ、ULK1 mRNA和蛋白表达显著升高,p-mTOR蛋白表达显著降低,差异有统计学意义(P<0.01)。补肾高剂量组大鼠软骨p-AMPK蛋白表达显著升高,差异有统计学意义(P<0.01)。结论:补肾强筋胶囊可能通过AMPK/mTOR通路上调KOA模型大鼠膝关节软骨自噬水平从而延缓病程。
Abstract:
To explore the effect and relative molecular mechanism of Bushenqiangjin capsule on knee osteoarthritis(KOA).Methods:40 SD rats were randomly divided into 4 groups,with 10 rats in each group.The KOA rat model was established by injection of papain and cysteine into joints.After 4 weeks,normal group and model group were given oral administration of normal saline,the high and low dose groups of bushenqiangjin capsule were given oral administration of 0.486 g/kg and 0.243 g/kg Bushenqiangjin capsule respectively.After 6 weeks,all the rats were killed,knee cartilage and serum were collected and tested.Hematoxylin-eosin(HE)staining and Safranin o-fast green staining were used to observe the pathological changes of knee cartilage in each group and modified Mankin score was calculated.The levels of serum interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in rats were detected by enzyme linked immunosorbent assay(ELISA).The mRNA and protein expressions of MMP13,Beclin1,LC3B,ULK1,p-AMPK and p-mTOR were detected by Real-time PCR and Western Blot.Results:Compared with the model group,the degeneration degree of knee cartilage and Mankin score of rats in the high and low dose groups of bushenqiangjin capsule were reduced significantly(P<0.01).Compared with the model group,the levels of serum IL-1β and TNF-α in the high and low dose groups of bushenqiangjin capsule were decreased significantly(P<0.01).Compared with the model group,the mRNA and protein expression of MMP13 were decreased significantly,the mRNA and protein expressions of Beclin1,LC3B-Ⅱ/Ⅰ and ULK1 were increased significantly,and the protein expression of p-mTOR was decreased significantly in the high and low dose groups of bushenqiangjin capsule(P<0.01).In addition,the protein expression of p-AMPK was increased significantly in the high dose group of bushenqiangjin capsule(P<0.01).Conclusion:Bushenqiangjin capsule may up-regulate the autophagy level of knee cartilage in KOA model rats through AMPK/mTOR pathway,which delays the course of KOA.

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备注/Memo

备注/Memo:
基金项目:广东省基础与应用基础研究基金项目(2018A030313584)广东省中医药局科研项目(20194002,20183001)
△通信作者 E-mail:xuxuemeng@163.com
更新日期/Last Update: 2021-04-15