[1]冷明昊 张卫华 陈东 廖银平.红曲通过激活腺甘酸活化蛋白激酶上调骨形态发生蛋白2表达对去卵巢大鼠骨质疏松症的改善作用[J].中国中医骨伤科杂志,2021,29(03):7-11.
　LENG Minghao ZHANG Weihua CHEN Dong LIAO Yinping.Red Yeast Rice Improves Osteoporosis in Ovariectomized Rats by Activating AMPK and Up-Regulating BMP2 Expression[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2021,29(03):7-11.
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红曲通过激活腺甘酸活化蛋白激酶上调骨形态发生蛋白2表达对去卵巢大鼠骨质疏松症的改善作用()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第29卷
期数:
2021年03期
页码:
7-11
栏目:
实验研究
出版日期:
2021-03-15

文章信息/Info

Title:
Red Yeast Rice Improves Osteoporosis in Ovariectomized Rats by Activating AMPK and Up-Regulating BMP2 Expression
文章编号:
1005-0205(2021)03-0007-05
作者:
冷明昊 张卫华 陈东 廖银平
1湖北随州市中心医院骨科(湖北 随州,441300)2武汉市汉阳医院骨一科3武汉科技大学医学院基础医学部△通信作者
Author(s):
LENG Minghao ZHANG Weihua CHEN Dong LIAO Yinping
1Department of Orthopedics,Suizhou Central Hospital,Suizhou 441300,Hubei China; 2Department of Orthopedics,Wuhan Hanyang Hospital,Wuhan 430050,China; 3Department of Basic Medicine,School of Medicine,Wuhan University of Science and Technology,Wuhan 430081,China.
关键词:
红曲;  腺甘酸活化蛋白激酶/骨形态发生蛋白2;  去卵巢大鼠;  骨质疏松症
Keywords:
red yeast rice;  AMPK/BMP2;  ovariectomized rats;  osteoporosis
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨红曲对去卵巢大鼠骨质疏松症的作用,以及对腺甘酸活化蛋白激酶(AMPK)信号通路和骨形态发生蛋白2(BMP2)表达的影响。方法:采取双侧卵巢切除手术构建骨质疏松症大鼠模型,给予造模成功的大鼠结合雌激素和质量分数浓度的红曲(1.56,3.12,6.24 g/kg)每天灌胃治疗,分为去卵巢组,雌激素组,红曲低、中、高剂量组,另设假手术组,每组6只。连续干预12周后,处死动物,称量大鼠体质量,双能X线测量骨密度,苏木精-伊红(HE)染色观察大鼠骨组织病理形态的改变,ELISA检测骨代谢的生物标志物,包括碱性磷酸酶(ALP)、酒石性酸性磷酸酶(TRAP)、骨钙素(OC)、Ⅰ型前胶原羧基端肽(PICP)水平,Western Blot法检测骨组织AMPK和BMP2的表达。结果:与假手术组相比,去卵巢组大鼠体质量降低,骨密度显著降低,骨小梁排列稀疏、紊乱并连接不完整,骨量减少,骨代谢的生物标志物ALP,TRAP,OC,PICP水平显著升高,下调了AMPK和BMP2的表达; 与去卵巢组相比,红曲各剂量组大鼠体质量增加,骨密度显著增加,骨小梁排列有所改善,骨小梁数目增加且连接增强,骨代谢的生物标志物ALP,TRAP,OC,PICP水平显著降低,激活AMPK信号通路并上调BMP2的表达; 其中红曲中剂量组变化最显著。结论:红曲增加了去卵巢大鼠的体质量和骨密度,改善了骨组织的形态和排列,降低了骨代谢标志物ALP,TRAP,OC,PICP水平,进而对骨质疏松症有改善作用,可能是通过激活AMPK信号通路上调BMP2来调控的。
Abstract:
To explore the effect of red yeast rice on osteoporosis from ovariectomized rats,as well as the effects on AMPK signaling pathway and BMP2 protein expression.Methods:Bilateral oophorectomy was used to construct a rat model of osteoporosis.Rats with successful modeling were given intragastric treatment combined with estrogen and different concentrations of red yeast(1.56,3.12,6.24 g/kg),which can be divided into ovary group,estrogen group,red,low,medium and high dose groups,another sham operation group with 6 animals in each group.After 12 weeks of continuous intervention,the animals were sacrificed,the rats were weighed.The bone density was measured by dual-energy X-ray.Hematoxylin-eosin(HE)staining was used to observe the pathological changes of bone tissue in rats,and the biomarkers of bone metabolism were detected by ELISA Substances including ALP,TRAP,OC,PICP levels.Western Blot was used to detect bone tissue AMPK and BMP2 protein expression.Results:Compared with the sham-operated group,the ovariectomized group had lower body weight,significantly lower bone density,sparse trabecular arrangement,disorder and incomplete connection,reduced bone mass,and levels of bone metabolism biomarkers ALP,TRAP,OC and lower PICP,down-regulated the expression of AMPK and BMP2.Compared with the ovariectomized group,the weight of the red yeast rice group increased significantly,and the bone density was significantly increased.The trabecular bone arrangement was improved,and the number of trabecular bone was increased.The connection was enhanced,and the levels of bone metabolism biomarkers ALP,TRAP,OC and PICP were significantly reduced.The AMPK signaling pathway and up-regulating the protein expression of BMP2 was activated.Among them,the middle dose group of red yeast rice had the most significant changes.Conclusion:Red yeast rice increases the weight and bone density of ovariectomized rats,improves the morphology and arrangement of bone tissue,and reduces the levels of bone metabolism markers ALP,TRAP,OC and PICP,which in turn can improve osteoporosis,which may be regulated by activating the AMPK signaling pathway to upregulate BMP2.

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备注/Memo

备注/Memo:
基金项目:湖北省卫生健康委员会创新团队项目(WJ2019M038)
更新日期/Last Update: 2021-03-15