[1]陈宇 张智 汪凡栋 宋昭君 刘元彬 唐龙 廖伟 郑佳状.超声微泡联合骨髓间充质干细胞移植对脊髓损伤大鼠的神经保护作用研究[J].中国中医骨伤科杂志,2021,29(03):1-6.
　CHEN Yu ZHANG Zhi WANG Fandong SONG Zhaojun LIU Yuanbin TANG Long LIAO Wei ZHENG Jiazhuang.The Neuroprotective Effect of Ultrasound Microbubbles Combined with Bone Marrow Mesenchymal Stem Cell Transplantation on Spinal Cord Injury Rats[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2021,29(03):1-6.
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超声微泡联合骨髓间充质干细胞移植对脊髓损伤大鼠的神经保护作用研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第29卷
期数:
2021年03期
页码:
1-6
栏目:
实验研究
出版日期:
2021-03-15

文章信息/Info

Title:
The Neuroprotective Effect of Ultrasound Microbubbles Combined with Bone Marrow Mesenchymal Stem Cell Transplantation on Spinal Cord Injury Rats
文章编号:
1005-0205(2021)03-0001-06
作者:
陈宇 张智 汪凡栋 宋昭君 刘元彬 唐龙 廖伟 郑佳状
1四川遂宁市中心医院脊柱外科(四川 遂宁,629000)△通信作者 E-mail:aabu123@163.com
Author(s):
CHEN Yu ZHANG Zhi WANG Fandong SONG Zhaojun LIU Yuanbin TANG Long LIAO Wei ZHENG Jiazhuang
1Spine Surgery,Suining Central Hospital Suining,Sichuan 629000,China.
关键词:
超声微泡;  骨髓间充质干细胞;  脊髓损伤;  神经保护;  炎症反应
Keywords:
ultrasound microbubbles;  bone marrow mesenchymal stem cells;  spinal cord injury;  neuroprotection;  inflammatory response
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨超声微泡联合骨髓间充质干细胞(BMSCs)移植对脊髓损伤大鼠的神经保护作用。方法:采用Allen’s 打击法构建SD大鼠脊髓损伤模型并分组进行对应处理,术后进行BBB评分,HE染色和尼氏染色检测脊髓组织与尼氏小体形态变化,ELISA检测炎症因子水平,免疫荧光染色和qRT-PCR检测神经相关因子与凋亡相关因子表达。结果:与模型组比较,超声微泡组、BMSCs组和超声微泡+BMSCs组术后第7天和第21天BBB评分增加,脊髓组织病理损伤减轻,尼氏小体形态得到改善,炎症反应减轻,Nestin,GFAP表达及BDNF,NT3,Bcl-2 mRNA表达升高,Bax,Caspase-3 mRNA表达下降,差异有统计学意义(P<0.05),且超声微泡和BMSCs联用效果更佳。结论:超声微泡联合BMSCs移植能够对脊髓损伤大鼠发挥神经保护的作用。
Abstract:
To investigate the neuroprotective effect of ultrasound microbubbles combined with bone marrow mesenchymal stem cells(BMSCs)transplantation on spinal cord injury rats.Methods:The SD rat spinal cord injury model was constructed by Allen’s strike method and divided into groups for corresponding treatment.After the operation BBB score was performed.HE staining and Nissl staining were used to detect the morphological changes of spinal cord tissue and Nissl body.ELISA was used to detect inflammatory factor levels,and immunofluorescence staining and qRT-PCR detects the expression of nerve-related factors and apoptosis-related factors.Results:Compared with the model group,the BBB scores in the ultrasound microbubble group,BMSCs group and ultrasound microbubble+BMSCs group increased on the 7th and 21th day after operation.The pathological damage of the spinal cord tissue was reduced,and the morphology of Nissl bodies was improved.Inflammation was reduced,Nestin,GFAP expression and BDNF,NT3,Bcl-2 mRNA expression increased,Bax and Caspase-3 mRNA expression decreased,and the combined effect of ultrasound microbubbles and BMSCs was better.Conclusion:Ultrasound microbubble combined with BMSCs transplantation exerts neuroprotective effect on spinal cord injury rats.

参考文献/References:

[1] REN H,CHEN X,TIAN M,et al.Regulation of inflammatory cytokines for spinal cord injury repair through local delivery of therapeutic agents[J].Adv Sci(Weinh),2018,5(11):1800529.
[2] YAO X,ZHANG Y,HAO J,et al.Deferoxamine promotes recovery of traumatic spinal cord injury by inhibiting ferroptosis[J].Neural Regen Res,2019,14(3):532-541.
[3] FOULAD DP,KORB MK,SKUPSKY H,et al.Crusted scabies triggering autonomic dysreflexia in a patient with spinal cord injury[J].JAAD Case Rep,2020,6(7):640-642.
[4] KAFKA J,LUKACOVA N,SULLA I,et al.Hypothermia in the course of acute traumatic spinal cord injury[J].Acta Neurobiol Exp,2020,80(2):172-178.
[5] IMURA T, TOMIYASU M, OTSURU N, et al. Hypoxic preconditioning increases the neuroprotective effects of mesenchymal stem cells in a rat model of spinal cord injury[J]. Journal of Stem Cell Research & Therapy, 2017, 7(7):1-7.
[6] BOUAKAZ A,ZEGHIMI A,DOINIKOV A A.Sonoporation:concept and mechanisms[J].Adv Exp Med Biol,2016,880:175-189.
[7] RIX A,CURAJ A,LIEHN E,et al.Ultrasound microbubbles for diagnosis and treatment of cardiovascular diseases[J].Semin Thromb Hemost,2020,46(5):545-552.
[8] CHOWDHURY S M,ABOU-ELKACEM L,LEE T,et al.Ultrasound and microbubble mediated therapeutic delivery:underlying mechanisms and future outlook[J].J Control Release,2020,326:75-90.
[9] YACOUB A,HAJEC M C,STANGER R,et al.Neuroprotective effects of perflurocarbon(oxycyte)after contusive spinal cord injury[J].J Neurotrauma,2014,31(3):256-267.
[10] BASSO D M,BEATTIE M S,BRESNAHAN J C.A sensitive and reliable locomotor rating scale for open field testing in rats[J].J Neurotraum,1995,12(1):1-21.
[11] 张月,周厚妊,辛莹,等.超声靶向微泡破坏技术介导基因转染的研究进展[J].临床超声医学杂志,2020,22(7):523-525.
[12] PERENO V,LEI J,CARUGO D,et al.Microstreaming inside model cells induced by ultrasound and microbubbles[J].Langmuir,2020,36(23):6388-6398.
[13] 薛静,张周龙,陈胜江.超声微泡联合干细胞移植对心肌梗死大鼠心肌微环境的影响及循环Irisin水平的相关性研究[J].中国循证心血管医学杂志,2019,11(4):448-451.
[14] KARKI R,KANNEGANTI T D.Diverging inflammasome signals in tumorigenesis and potential targeting[J].Nat Rev Cancer,2019,19(4):197-214.
[15] PATERNITI I,CAMPOLO M,CORDARO M,et al.PPAR-alpha modulates the anti-inflammatory effect of melatonin in the secondary events of spinal cord injury[J].Mol Neurobiol,2017,54(8):5973-5987.
[16] OKADA S.The pathophysiological role of acute inflammation after spinal cord injury[J].Inflamm Regen,2016,36:20.
[17] NIAZI I F,LYLE M A,RISING A,et al.Redistribution of inhibitory force feedback between a long toe flexor and the major ankle extensor muscles following spinal cord injury[J].J Neurosci Res,2020,98(8):1646-1661.
[18] 姬文晨,蒋婉婷,马兴,等.稳定过表达 BDNF 和 NT-3 基因的脂肪干细胞系的建立及其意义[J].西安交通大学学报(医学版),2019,40(3):393-398.
[19] CHEN B,WANG G,LI W,et al.Memantine attenuates cell apoptosis by suppressing the calpain-caspase-3 pathway in an experimental model of ischemic stroke[J].Exp Cell Res,2017,351(2):163-172.

备注/Memo

备注/Memo:
基金项目:四川省卫生计生委科研课题(16PJ523)
更新日期/Last Update: 2021-03-15