[1]吴建军△ 梁珪清 郑昌坤 徐强 徐佳隆.黄连素对脊髓损伤大鼠染色病理、骨形态发生蛋白表达及神经元活性的影响[J].中国中医骨伤科杂志,2021,29(02):5-9.
　WU Jianjun LIANG Guiqing ZHENG Changkun XU Qiang XU Jialong.Study of BBR on HE Pathology,BMP-4 mRNA Expression andNeuronal Activity in Rats with Spinal Cord Injury[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2021,29(02):5-9.
点击复制

黄连素对脊髓损伤大鼠染色病理、骨形态发生蛋白表达及神经元活性的影响()
分享到：

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第29卷
期数:
2021年02期
页码:
5-9
栏目:
实验研究
出版日期:
2021-02-15

文章信息/Info

Title:
Study of BBR on HE Pathology,BMP-4 mRNA Expression andNeuronal Activity in Rats with Spinal Cord Injury
文章编号:
1005-0205(2021)02-0005-05
作者:
吴建军1△ 梁珪清1 郑昌坤1 徐强2 徐佳隆2
1厦门大学附属福州第二医院(福州,350007)2福建中医药大学
Author(s):
WU Jianjun1△ LIANG Guiqing1 ZHENG Changkun1 XU Qiang2 XU Jialong2
1Fuzhou Second Hospital Affiliated of Xiamen University,Fuzhou 350007,China; 2Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China.
关键词:
黄连素;  神经元活性;  骨形态发生蛋白4(BMP-4);  脊髓损伤
Keywords:
berberine(BBR);  neuronal activity;  bone morphogenetic protein 4(BMP-4);  spinal cord injury
分类号:
R-33
文献标志码:
A
摘要:
目的:研究黄连素对脊髓损伤大鼠苏木精-伊红(HE)病理、骨形态发生蛋白4(BMP-4)mRNA表达及神经元活性的影响。方法:将广东省医学SD大鼠中心的大鼠随机分为脊髓损伤组(S组)、健康对照组(H组)、黄连素组(B组),每组6只。采用流式细胞法、TUNEL法、HE染色法、免疫组化法、qRT-PCR法检测S组、B组、H组大鼠脊髓细胞凋亡数量、脊髓细胞病变程度、脊髓细胞中BMP-4蛋白阳性表达、脊髓细胞中BMP-4 mRNA的表达量、脊髓细胞凋亡率的差异性来探究黄连素对脊髓损伤大鼠HE病理、BMP-4 mRNA表达及神经元活性的影响。结果:S组脊髓细胞凋亡数量显著多于B组(P<0.05),与H组相比较,B组脊髓细胞凋亡数量显著多于H组,差异有统计学意义(P<0.05)。B组神经元细胞的凋亡情况与S组相比较显著减少,差异有统计学意义(P<0.05)。与S组比较,B组大鼠脊髓水肿减轻,损伤程度有好转趋势,细胞坏死数目有所减少。与S组相比较,B组脊髓细胞中BMP-4阳性数显著降低,差异有统计学意义(P<0.05)。与B组相比较,H组细胞BMP-4阳性数显著下降,差异有统计学意义(P<0.05); 与S组相比较,B组大鼠BMP-4 mRNA表达量显著降低,BMP-2 mRNA表达量显著上升,差异有统计学意义(P<0.05); 与B组相比较,H组BMP-4 mRNA表达量显著降低,BMP-2 mRNA表达量显著上升,差异有统计学意义(P<0.05)。结论:黄连素对脊髓损伤大鼠具有一定治疗作用,黄连素干预后能够显著减少大鼠脊髓细胞的凋亡数量,降低凋亡率,同时改善大鼠脊髓细胞的病理形态,促进康复,黄连素对脊髓损伤大鼠的作用机制可能与下调脊髓细胞中BMP-4的表达有关。
Abstract:
To study the effects of berberine(BBR)on hematoxylin eosin(HE)pathology,bone morphogenetic protein 4(BMP-4)mRNA expression and neuronal activity in rats with spinal cord injury.Methods: The rats in the Guangdong medical SD rat center were randomly divided into spinal cord injury group(S group); healthy control group(H group); berberine group(B group),each with 6 rats. Flow cytometry,TUNEL method,HE staining method,immunohistochemistry method,and qRT-PCR method were used to detect the number of spinal cord cell apoptosis,the degree of spinal cord cytopathy,the expression BMP-4 protein and mRNA in spinal cord cells and the difference in the apoptosis rate of spinal cord cells in groups S,B,and H to explore the effects of berberine(BBR)on HE pathology,BMP-4 mRNA expression and neuronal activity in rats with spinal cord injury. Results:The number of apoptotic cells in B group was significantly more than that in S group(P<0.05). The apoptosis of neurons in B group was significant less than that in H group(P<0.05). Compared with S group,the spinal cord edema in B group was alleviated,the degree of injury tended to improve,and the number of cell necrosis decreased.Compared with S group,the number of BMP-4 positive cells in B group was significantly lower than S group(P<0.05). Compared with B group,the number of BMP-4 positive cells in H group was significantly decreased(P<0.05). Compared with S group,the expression of BMP-4 mRNA and BMP-2 mRNA in B group were significantly lower than S group(P<0.05). Compared with B group,the expression of BMP-4 mRNA and BMP-2 mRNA in H group were significantly decreased and significantly increased(P<0.05). Conclusion:Berberine(BBR)has a certain therapeutic efficacy for spinal cord injury of rats. The number of rat spinal cord apoptosis can be significantly reduced after BBR intervention,and the rate of apoptosis is reduced. At the same time,berberine(BBR)can improve the pathological morphology of rat spinal cord cells and promote recovery. The mechanism of BBR on spinal cord injury rats may be related to down-regulation of bone morphogenetic protein 4(BMP-4)expression in spinal cord cells.

参考文献/References:

[1] MA J K,MCCRACKEN L A,VOSS C,et al.Physical activity measurement in people with spinal cord injury:comparison of accelerometry and self-report(the physical activity recall assessment for people with spinal cord injury)[J].Disability & Rehabilitation,2020,42(2):240-246.
[2] PRZYDACZ M,CHLOSTA P,CORCOS J.Recommendations for urological follow-up of patients with neurogenic bladder secondary to spinal cord injury[J].Int Urol Nephrol,2018,50:1005-1016.
[3] 王军建,刘鑫,刘方娜,等.孕期尼古丁暴露所致子代成骨细胞BMP-4/Smad信号通路的变化[J].医学研究杂志,2018,47(11):63-67.
[4] 李珏丹,王敏,代泉,等.骨形态发生蛋白-4和-7对人牙周膜干细胞增殖和成骨分化的影响[J].山西医科大学学报,2019,50(5):614-620.
[5] ZHANG T,NI S F, LUO Z X,et al.The protective effect of microRNA-21 in neurons after spinal cord injury[J].Spinal Cord,2019,57:141-149.
[6] SUN G D,LI G Q,LI D H,et al.hucMSC derived exosomes promote functional recovery in spinal cord injury mice via attenuating inflammation[J].Materials Science& Engineering,C:Materials for Biogical Applications,2018,89:194-204.
[7] WANG T,FANG X,YIN Z S.Endothelial progenitor cell-conditioned medium promotes angiogenesis and is neuroprotective after spinal cord injury[J].Neural Regeneration Research,2018,52(53):1439-1447.
[8] 王奕,于荣华,徐炜,等.黄连素在脊髓损伤中对线粒体的保护作用及机制[J].脊柱外科杂志,2019,17(2):121-126.
[9] 岳旭珂,刘合玉,夏彬,等.黄连素对大鼠脊髓损伤后细胞自噬与凋亡的影响[J].中华实验外科杂志,2015,23(32):1516-1518.
[10] 王臻,丁慧,杨瑞.黄连素激活核因子E2相关因子/血红素氧合酶-1增强内皮细胞抗过氧化氢损伤[J].中国体外循环杂志,2019,17(1):48-54.
[11] 柴红,吴平亚,杨政,等.黄连素对大鼠结肠敏感性和动力学异常引起肠易激综合征的影响及其机制探讨[J].微循环学杂志,2019,43(4):20-25.
[12] 谭文倩,李锐,于鑫,等.低剂量黄连素对小鼠肠道短链脂肪酸和胆汁酸成分以及血脂的影响[J].复旦学报(医学版),2020,46(78):864-869.
[13] 魏晓,侯静,吴蔚桦,等.血清BMP-2、BMP-4检测在慢性肾脏病大鼠主动脉钙化中的意义[J].中国动脉硬化杂志,2018,13(9):89-92.
[14] 白广超,金宏亮,雷堃,等.骨形态发生蛋白7促进大鼠急性脊髓损伤后神经功能修复的研究[J].新疆医学,2018,48(11):1153-1157.
[15] 王莹,王辉,杨益民,等.脊髓损伤大鼠膝关节软骨IL-1、BMP-2表达及肢体功能评价[J].山西医科大学学报,2019,50(5):66-72.
[16] 张玉玲,白广超,金宏亮,等.BMP7蛋白对大鼠急性脊髓损伤后神经元修复作用的研究[J].生物骨科材料与临床研究,2018,15(4):7-11.
[17] 吴茉莉,李萍,汪黎鸿,等.骨形态发生蛋白4对反应性星形胶质细胞增殖的影响[J].大连医科大学学报,2015,37(4):328-330.
[18] 赵鹏,饶耀剑,崔泽升,等.补阳还五汤对BMP2/4介导轴突再髓鞘化的影响[J].中国中医药现代远程教育,2015,13(3):144-145.
[19] 马敏杰,贺西京,王国毓,等.大鼠脊髓损伤后骨形态发生蛋白2表达变化的规律及意义[J].中国脊柱脊髓杂志,2011,13(4):329-333.

备注/Memo

备注/Memo:
基金项目:福建省自然科学基金项目(2018J01364)
△通信作者 E-mail:wjj7101522@163.com
更新日期/Last Update: 2021-02-15