[1]浦飞飞 陈凤霞 邵增务△ 刘建湘 张志才.白花蛇舌草总黄酮对骨肉瘤小鼠免疫相关因子的调节作用[J].中国中医骨伤科杂志,2021,29(02):1-4.
　PU Feifei CHEN Fengxia SHAO Zengwu LIU Jianxiang ZHANG Zhicai.Effect of Total Flavones of Oldenlandia Diffusa on Immune-RelatedFactors in Mouse Model of Osteosarcoma[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2021,29(02):1-4.
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白花蛇舌草总黄酮对骨肉瘤小鼠免疫相关因子的调节作用()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第29卷
期数:
2021年02期
页码:
1-4
栏目:
实验研究
出版日期:
2021-02-15

文章信息/Info

Title:
Effect of Total Flavones of Oldenlandia Diffusa on Immune-RelatedFactors in Mouse Model of Osteosarcoma
文章编号:
1005-0205(2021)02-0001-04
作者:
浦飞飞1; 2 陈凤霞3 邵增务1△ 刘建湘1 张志才1
1华中科技大学同济医学院附属协和医院骨科(武汉,430022)2华中科技大学同济医学院附属武汉中西医结合医院骨科3武汉大学中南医院肿瘤放化疗科
Author(s):
PU Feifei1; 2 CHEN Fengxia3 SHAO Zengwu1△ LIU Jianxiang1 ZHANG Zhicai1
1Department of Orthopedics,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China; 2Department of Orthopedics,Wuhan Integrated TCM & Western Medicine Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China; 3Department of Radiation Oncology and Medical Oncology,Zhongnan Hospital,Wuhan University,Wuhan 430071,China.
关键词:
白花蛇舌草;  总黄酮;  骨肉瘤;  免疫相关因子
Keywords:
oldenlandia diffusa;  total flavones;  osteosarcoma;  immune-related factors
分类号:
R-33
文献标志码:
A
摘要:
目的:研究白花蛇舌草总黄酮(FOD)对骨肉瘤胫骨原位移植瘤小鼠模型免疫相关因子的调节作用。方法:选用40只SPF级4～6周龄18～20 g的BALB/c小鼠,雌雄各半。构建骨肉瘤胫骨原位移植瘤小鼠模型,随机分为空白对照组和FOD组,每组各20只。按照分组干预2周时间,观察小鼠的一般状况,处死小鼠后检测胸腺指数和脾脏指数,采用流式细胞仪检测淋巴细胞亚群,ELISA法检测小鼠血清免疫相关因子水平。结果:与空白对照组相比,FOD组小鼠胸腺指数和脾脏指数显著增加,T淋巴细胞亚群(CD+4,CD+8,CD+4/CD+8)比例显著升高,免疫相关因子IL-2,TNF-α和IFN-γ显著升高,IL-4和IL-6显著降低,各组数据差异均有统计学意义(P<0.05)。结论:FOD能够调节骨肉瘤胫骨原位移植瘤小鼠模型免疫相关因子表达,可为骨肉瘤的综合治疗提供新的治疗手段。
Abstract:
To study the effect of total flavone of oldenlandia diffusa(FOD)on immune-related factors in mouse model of osteosarcoma tibial orthotopic transplantation tumor.Methods:40 BALB/c mice with SPF grades of 4 to 6 weeks and weighing 18 to 20 g were selected,half male and half female.Mouse models of osteosarcoma tibial orthotopic xenograft tumor were established and randomly divided into blank control group and FOD group,with 20 mice in each group.The general conditions of the mice were observed after 2 weeks of intervention according to the grouping conditions.Thymic index and spleen index were detected after the mice were killed,lymphocyte subsets were detected by flow cytometry,and serum immune-related factors of the mice were detected by ELISA.Results:Compared with the blank control group,the thymus and spleen index of mice in FOD group were significantly increased,the T lymphocyte subsets(CD+4,CD+8,CD+4/CD+8)rate significantly increased,the serum immune-related factors IL-2,TNF-α and IFN-γ were significantly increased,while IL-4 and IL-6 were significantly decreased,and the data of each group were statistically different(P<0.05).Conclusion:FOD can regulate the expression of immune-related factors in the mouse model of osteosarcoma tibial orthotopic xenograft tumor,this study provid a new treatment method for osteosarcoma.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(81904231)中国博士后科学基金项目(2020M672369)湖北省博士后创新实践岗位项目(34)武汉市医学科研项目(WZ18Q05)
△通信作者 E-mail:shaozengwupro@163.com
更新日期/Last Update: 2021-02-15