[1]高伟华 李玉杰 杨鸿冉 李志超 杨振国 齐鲁 董建文△ 王文波△.加味阳和汤对膝骨性关节炎软骨细胞凋亡的影响研究[J].中国中医骨伤科杂志,2021,29(01):1-5.
　GAO Weihua LI Yujie YANG Hongran LI ZhichaoYANG Zhenguo QI Lu DONG Jianwen WANG Wenbo.Study on the Apoptosis Mechanism of Jiawei YangheDecoction on Chondrocytes in Osteoarthritis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2021,29(01):1-5.
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加味阳和汤对膝骨性关节炎软骨细胞凋亡的影响研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第29卷
期数:
2021年01期
页码:
1-5
栏目:
实验研究
出版日期:
2021-01-15

文章信息/Info

Title:
Study on the Apoptosis Mechanism of Jiawei YangheDecoction on Chondrocytes in Osteoarthritis
文章编号:
1005-0205(2021)01-0001-05
作者:
高伟华1 李玉杰1 杨鸿冉1 李志超1 杨振国1 齐鲁1 董建文1△ 王文波1△
Author(s):
GAO Weihua1 LI Yujie1 YANG Hongran1 LI Zhichao1YANG Zhenguo1 QI Lu1 DONG Jianwen1△ WANG Wenbo1△
1The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250001,China.
关键词:
膝骨关节炎;  加味阳和汤;  凋亡;  骨形态发生蛋白-2;  Sry相关组蛋白9
Keywords:
knee osteoarthritis;  Jiawei Yanghe decoction;  apoptosis;  bone morphogenetic protein-2(BMP2);  Sry-related HMG-box gene 9(Sox9)
分类号:
R684.3
文献标志码:
A
摘要:
目的:观察加味阳和汤对膝骨关节炎兔关节软骨骨形态发生蛋白-2(BMP2)及Sry相关组蛋白9(Sox9)表达的影响。方法:将健康新西兰大白兔随机分为正常组、模型组和实验组。除正常组外,其余2组动物均按照改良赫尔特法(Hulth法)制作膝骨关节炎模型后,分组处理,4,6,8周后取材,制作标本,行免疫组化染色观察Ⅱ型胶原(Col Ⅱ)的变化、 实时聚合酶链反应(PCR)监测软骨组织中BMP2和Sox9的表达情况、TUNEL监测软骨组织中软骨细胞凋亡情况。结果:正常组Ⅱ型胶原的表达量最高,模型组表达量最少,骨性关节炎时间越长阳性染色越浅,同一时间段内实验组染色较好,且分布较均匀; BMP2在正常软骨中少量表达,而在骨性关节炎软骨中有大量表达,骨性关节炎时间越久,BMP2的表达量越多,同一时间段内实验组的BMP2的表达量比模型组低; 而Sox9在正常组中有大量表达,而在模型组中表达量明显减少,在同一时间段内实验组均较模型组的表达量增高。正常组软骨中凋亡的软骨细胞数量较少,而在模型组中同一时间段内软骨细胞凋亡数量较正常组明显增加,骨性关节炎时间越久凋亡细胞数量越多,实验组经治疗后细胞调亡数量减少。结论:加味阳和汤能够下调膝关节中BMP2的表达量,上调Sox9的表达量,从而减缓骨性关节炎软骨退变,促进Ⅱ型胶原的表达,进而减少软骨细胞的凋亡。
Abstract:
To observe the effect of Jiawei Yanghe decoction on the expression of bone morphogenetic protein-2(BMP2)and Sry-related HMG-box gene 9(Sox9)in articular cartilage of rabbits with knee osteoarthritis.Methods:Healthy New Zealand white rabbits were randomly divided into normal group,model group and experimental group.In addition to the normal group,knee osteoarthritis model was made according to the modified Hulth method,after 4,6 and 8 weeks of treatment,samples were taken and specimens were made,the changes of typeⅡcollagen(Col Ⅱ)were observed by immunohistochemistry,the expression of BMP2 and Sox9 in cartilage was monitored by PCR,and the apoptosis of chondrocytes was monitored by TUNEL.Results:The expression of type Ⅱ collagen was the highest in the normal group,the least in the model group,the longer the osteoarthritis time,the lighter the positive staining,the staining in the experimental group was better in the same period,and more evenly distributed.BMP2 was scarcely present in normal articular cartilage,but there was a lot of expression in osteoarthritis,the longer the osteoarthritis time,the more BMP2 expression,and the expression of BMP2 in the experimental group was lower than that in the model group in the same period.However,Sox9 was largely expressed in normal articular cartilage,but significantly reduced in the model group,and amount of expression in the experimental group was higher than that in the model group in the same period.The number of apoptotic chondrocytes in the cartilage of the normal group was little,but the number of apoptotic chondrocytes in the same period of time in the model group was significantly higher than that in the normal group,the number of apoptosis increased with osteodystrophy time,and the number of apoptosis decreased in the experimental group after treatment.Conclusion:Jiawei Yanghe decoction can decrease the expression of BMP2 and up regulate the expression of Sox9 in knee joint,which can slow down the degeneration of osteoarthritis cartilage,promote the expression of type Ⅱ collagen,and reduce the apoptosis of chondrocytes.

参考文献/References:

[1] 杨鸿冉,董建文,李志超,等.加味阳和汤治疗膝骨性关节炎临床研究[J].云南中医中药杂志,2014,35(4):8-10.
[2] 王文波,董建文,杨振国,等.加味阳和汤对早期膝骨关节炎兔关节软骨的影响[J].中医正骨,2015,27(1):1-4.
[3] 高伟华,李玉杰,杨鸿冉,等.加味阳和汤对不同时期膝骨关节炎兔关节软骨结构的影响[J].中国中医骨伤科杂志,2019,27(5):7-13.
[4] SHI S,WANG C,ACTON A J,et al.Role of sox9 in growth factor regulation of articular chondrocytes[J].J Cell Biochem,2015,116(7):1391-400.
[5] 刘献祥,李西海,周江涛.改良Hulth造模法复制膝骨性关节炎的实验研究[J].中国中西医结合杂志,2005,25(12):1104-1108.
[6] 区广鹏,肖军,郑佐勇,等.老年膝关节骨性关节炎患病危险因素的调查[J].中国组织工程研究,2012,16(50):9463-9470.
[7] LOESER R.Osteoarthritis:a disease of the joint as an organ[J].Arthritis Rheum,2012,64(6):1697-1707.
[8] HALL A C.The role of chondrocyte morphology and volume in controlling phenotype-implications for osteoarthritis,cartilage repair,and cartilage engineering[J].Current Rheumatology Reports,2019,21(8):38.
[9] 张清,杨功旭,钟传棋,等.基于数据挖掘的杨功旭治疗膝关节骨性关节炎用药规律研究[J].中国中医骨伤科杂志,2018,26(9):46-49.
[10] 彭时雨,谢雁鸣,黎元元,等.基于文献计量学方法对中医药治疗膝关节骨性关节炎主要流派的研究[J].中国中医骨伤科杂志,2016,24(9):36-41.
[11] LEFEBVRE V,DVIR-GINZBERG M.Sox9 and the many facets of its regulation in the chondrocyte lineage[J].Connective Tissue Research,2016,58(1):2-14.
[12] ZEHENTNER B K,DONY C,BURTSCHER H.The transcription factor Sox9 is involved in BMP-2 signaling[J].J Bone Miner Res,1999,14(10):1734-1741.
[13] GAMER L W,PREGIZER S,GAMER J,et al.Role of BMP2 in the maturation and maintenance of the knee joint[J].J Bone Miner Res,2018,33(9):1708-1717.
[14] VAN DER KRAAN P M,BLANEY DAVIDSON E N,VAN DEN BERG W B.Bone morphogenetic proteins and articular cartilage:to serve and protect or a wolf in sheep clothing’s?[J].Osteoarthritis Cartilage,2010,18(6):735-741.
[15] NAKASE T,MIYAJI T,TOMITA T,et al.Localization of bone morphogenetic protein-2 in human osteoarthritic cartilage and osteophyte[J].Osteoarthritis and Cartilage,2003,11(4):278-284.
[16] YIN W.Correlation of bone morphogenetic protein-2 levels in serum and synovial fluid with disease severity of knee osteoarthritis[J].Med Sci Monit,2015,21:363-370.
[17] BLANEY DAVIDSON E N,VITTERS E L,BENNINK M B,et al.Inducible chondrocyte-specific overexpression of BMP2 in young mice results in severe aggravation of osteophyte formation in experimental OA without altering cartilage damage[J].Ann Rheum Dis,2015,74(6):1257-1264.
[18] 刘艳,尹若峰,侯睿智.BMP-2诱导软骨细胞凋亡及增殖的研究[J].中华骨与关节外科杂志,2016,9(3):251-254.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(81503594,81800740,81173286)济南市科技发展计划项目(201102063)中国博士后基金项目(2018M630761)
1山东中医药大学第二附属医院(济南,250001)
△通信作者 E-mail:dongjianwen2006@sina.com(董建文)wangvenbo@126.com(王文波)
更新日期/Last Update: 2021-01-15