[1]洪建仁 任翠花 姜砚劼 柯利平△.利拉鲁肽通过抑制线粒体依赖性凋亡及缺氧诱导因子1α表达对脊髓损伤的保护作用及其机制[J].中国中医骨伤科杂志,2020,28(11):1-5.
　HONG Jianren REN Cuihua JIANG Yanjie KE Liping.iraglutide Protects Spinal Cord Injury via SuppressingMitochondrial-dependent Apoptosis and HIF-1α Expression[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2020,28(11):1-5.
点击复制

利拉鲁肽通过抑制线粒体依赖性凋亡及缺氧诱导因子1α表达对脊髓损伤的保护作用及其机制()
分享到：

《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第28卷
期数:
2020年11期
页码:
1-5
栏目:
实验研究
出版日期:
2020-11-10

文章信息/Info

Title:
iraglutide Protects Spinal Cord Injury via SuppressingMitochondrial-dependent Apoptosis and HIF-1α Expression
文章编号:
1005-0205(2020)11-0001-05
作者:
洪建仁1 任翠花2 姜砚劼1 柯利平1△
1湖北黄石市第四医院骨科(湖北 黄石,435006)2黄石市第四医院功能科
Author(s):
HONG Jianren1 REN Cuihua2 JIANG Yanjie1 KE Liping1△
1Department of Orthopedics, the Fourth Hospital of Huangshi, Huangshi 435006,Hubei China; 2Department of Functional, the Fourth Hospital of Huangshi, Huangshi 435006,Hubei China.
关键词:
利拉鲁肽;  脊髓损伤;  线粒体依赖性凋亡;  缺氧诱导因子1α
Keywords:
Liraglutide;  spinal cord injury;  mitochondrial-dependent apoptosis;  HIF-1α
分类号:
R-33
文献标志码:
A
摘要:
目的：探索利拉鲁肽(Lir)对脊髓损伤(SCI)大鼠的保护作用及相关机制。方法:36只SD大鼠随机分为对照组、SCI组和SCI+Lir组,每组各12只,术后利用BBB评分评价大鼠下肢运动功能,ELISA法检测脊髓组织中Smac/Diablo、细胞色素c(Cyt-c)、缺氧诱导因子1α(HIF-1α)、凋亡相关因子配体(FASL)、肿瘤坏死因子α(TNF-α)及Caspase3表达,Western Blot法检测凋亡相关蛋白Smac/Diablo,Cyt-c,P53,Caspase3,Bax,Bcl-2以及HIF-1α,FASL,利用组织学染色观察脊髓组织变化。结果:BBB评分显示利拉鲁肽14 d后开始能明显改善大鼠下肢运动功能; ELISA结果显示利拉鲁肽能显著抑制脊髓损伤后Smac/Diablo,Cyt-c,P53,HIF-1α,FASL,TNF-α及Caspase3表达; Western Blot法检测结果显示利拉鲁肽能明显逆转脊髓损伤后凋亡蛋白Smac/Diablo,Cyt-c,P53,Caspase3,Bax,Bcl-2表达,抑制HIF-1α及FASL表达。结论:利拉鲁肽可通过抑制线粒体依赖性凋亡及HIF-1α表达促进脊髓损伤的恢复。
Abstract:
Objective:To explore the protective effect of Liraglutide(Lir)on spinal cord injury(SCI)in rats and its related mechanism. Methods:36 SD rats were divided into 3 groups: the control group(n=12), SCI group(n=12)and SCI+Lir group(n=12). Lower limb motor function was valued via BBB score, and the content of Smac/Diablo, Cytochrome C(Cyt-c), hypoxia-inducible factor-1α(HIF-1α), apoptosis-related factor ligand(FASL), tumor necrosis factor-α(TNF-α)and caspase-3 were detected by ELISA. Western Blot was used to detect the apoptosis-related proteins, such as Smac/Diablo, Cyt-c, p53, caspase-3, Bax, Bcl-2 and HIF-1α, FASL were also valued. Changes of the spinal cord were observed by histological staining. Results:BBB score revealed that Liraglutide could significantly improve the lower limb motor function since 14th day. ELISA demonstrated that Liraglutide could markedly suppress the protein expression of Smac/Diablo, Cyt-c, p53, HIF-1α, FASL, TNF-α and caspase-3. Western Blot revealed that Liraglutide could significantly decrease the apoptosis-related proteins, such as Smac/Diablo, Cyt-c, p53, caspase-3, Bax, Bcl-2 and HIF-1α, FASL expression. Conclusion:Liraglutide can protect spinal cord injury probably via suppressing mitochondrial-dependent apoptosis and HIF-1α expression.

参考文献/References:

[1] WANG J Y,LI H Y Z,REN Y C,et al.Local delivery of β-elemene improves locomotor functional recovery by alleviating endoplasmic reticulum stress and reducing neuronal apoptosis in rats with spinal cord injury[J].Cell Physiol Biochem,2018,49(2):595-609.
[2] 刘伟,陈武桂,孙靖,等.完全性脊髓损伤后脊髓组织病理学变化及其意义[J].中华创伤杂志,2018,34(2):138-144.
[3] 汪九龄,何波,储瑞亮,等.大鼠急性脊髓损伤后肾脏的病理变化及其意义[J].中华创伤杂志.2017,33(5):459-464.
[4] KANEKO S,UEDA Y,TAHARA Y.GLP1 receptor agonist Liraglutide is an effective therapeutic option for perioperative glycemic control in type 2 diabetes within enhanced recovery after surgery(ERAS)protocols[J].Eur Surg Res,2018,59(5/6):349-360.
[5] CHEN J,WANG Z,MAO Y,et al.Liraglutide activates autophagy via GLP-1R to improve functional recovery after spinal cord injury[J].Oncotarget,2017,8(49):85949-85968.
[6] MENG X L,HAI Y,ZHANG X N,et al.Hyperbaric oxygen improves functional recovery of rats after spinal cord injury via activating stromal cell-derived factor-1/CXC chemokine receptor 4 axis and promoting brain-derived neurothrophic factor expression[J].Chin Med J(Engl),2019,132(6):699-706.
[7] KARAOZ E,TEPEKOY F,YILMAZ I,et al.Reduction of inflammation and enhancement of motility after pancreatic islet derived stem cell transplantation following spinal cord injury[J].J Korean Neurosurg Soc,2019,62(2):153-165.
[8] ZHANG H,LI D,ZHANG Y,et al.Knockdown of lncRNA BDNF-AS suppresses neuronal cell apoptosis via downregulating miR-130b-5p target gene PRDM5 in acute spinal cord injury[J].RNA Biol,2018,15(8):1071-1080.
[9] LI Y,DU J,ZHU E,et al.Liraglutide suppresses proliferation and induces adipogenic differentiation of 3T3-L1 cells via the Hippo-YAP signaling pathway[J].Mol Med Rep,2018,17(3):4499-4507.
[10] ZHU H,ZHANG Y,SHI Z,et al.The neuroprotection of Liraglutide against ischaemia-induced apoptosis through the activation of the PI3K/AKT and MAPK pathways[J].Sci Rep,2016,6:26859.
[11] YU W R,FEHLINGS M G.Fas/FASL-mediated apoptosis and inflammation are key features of acute human spinal cord injury:implications for translational,clinical application[J].Acta Neuropathol,2011,122(6):747-761.
[12] YIN V,MIAN S H,KONERMANN L.Lysine carbonylation is a previously unrecognized contributor to peroxidase activation of cytochrome c by chloramine-T[J].Chem Sci,2019,10(8):2349-2359.
[13] GONG Z M,TANG Z Y,SUN X L.miR-411 suppresses acute spinal cord injury via downregulation of Fas ligand in rats[J].Biochem Biophys Res Commun,2018,501(2):501-506.
[14] CHEN H,ZHENG J,MA J.Vanillin ameliorates changes in HIF-1α expression and neuronal apoptosis in a rat model of spinal cord injury[J].Restor Neurol Neurosci,2019,37(1):21-29.
[15] CHEN H,LI J,LIANG S,et al.Effect of hypoxia-inducible factor-1/vascular endothelial growth factor signaling pathway on spinal cord injury in rats[J].Exp Ther Med,2017,13(3):861-866.

备注/Memo

备注/Memo:
(收稿日期:2020-04-17)△通信作者 E-mail:1871646288@qq.com
更新日期/Last Update: 2020-11-10