[1]赵宝峰 张军 余磊 齐明 孙冶智 梁宏伟△.骨炎方治疗骨感染作用机制的实验研究[J].中国中医骨伤科杂志,2020,28(09):12-15.
　ZHAO Baofeng ZHANG Jun YU Lei QI Ming SUN Yezhi LIANG Hongwei.Mechanisms of Guyan Prescription Research on Experimental Osteomyelitis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2020,28(09):12-15.
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骨炎方治疗骨感染作用机制的实验研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第28卷
期数:
2020年09期
页码:
12-15
栏目:
实验研究
出版日期:
2020-09-10

文章信息/Info

Title:
Mechanisms of Guyan Prescription Research on Experimental Osteomyelitis
文章编号:
1005-0205(2020)09-0012-04
作者:
赵宝峰1 张军1 余磊1 齐明1 孙冶智1 梁宏伟1△
1武警北京市总队医院外二科(北京,100027)
Author(s):
ZHAO Baofeng1 ZHANG Jun1 YU Lei1 QI Ming1 SUN Yezhi1 LIANG Hongwei1△
1The Second Surgical Department,Hospital of Beijing Armed Police Forces,Beijing 100027,China.
关键词:
骨炎方;  兔;  慢性骨髓炎;  炎症反应;  促炎/抗炎细胞因子
Keywords:
Guyan prescription;  rabbit;  chronic osteomyel-itis;  inflammatory response;  proinflammatory and anti-inflammatory cytokines
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨中药复方骨炎方对家兔慢性骨髓炎的抗炎效应。方法:采用家兔右胫骨骨髓腔内注射金黄色葡萄球菌的方式以诱导慢性骨髓炎模型,动物分为模型组、阳性药克林霉素组(60 mg/kg)、骨炎方两个剂量组(生药量分别为3.6和1.8 g/kg)以及假手术组。各组灌胃给药,分别于给药后第2,4,6周对右胫骨行X线片检查并予诊断分型,采血测定白细胞计数和血沉,取血清测定白介素(IL)-1β,IL-4,IL-6,IL-10等细胞因子含量。结果:骨炎方高剂量组第2,4,6周的X线片诊断分型与模型组比较差异有统计学意义(P<0.01); 骨炎方低剂量组第4,6周的X线片诊断分型与模型组比较差异有统计学意义(P<0.05)。与模型组比较,骨炎方高、低剂量组白细胞计数和血沉在给药后第2,4,6周均有明显降低,差异有统计学意义(P<0.05)。与模型组比较,骨炎方高、低剂量组第2,4,6周的促炎细胞因子IL-1β和IL-6水平均有明显降低,差异有统计学意义(P<0.05),第6周的抗炎细胞因子IL-4水平有明显增高,差异有统计学意义(P<0.01); 高剂量组抗炎细胞因子IL-10水平则在第4,6周有明显增高,差异有统计学意义(P<0.05)。结论:骨炎方抗家兔慢性骨髓炎的作用机制可能与抑制致病菌、控制炎症反应、恢复促炎/抗炎细胞因子平衡等有关。
Abstract:
Objective:To investigate the anti-inflammatory effect of Guyan prescription against rabbits with chronic osteomyelitis(COM).Methods:Injection of staphylococcus aureus in the right tibia was used to established the experimental model of COM.Then rabbits with COM were divided randomly into 5 groups:model,clindamycin(60 mg/kg),Guyan prescription high and low dose(3.6 and 1.8 g/kg,respectively)and sham groups.Animals were treated by oral administration for 6 weeks.The X-ray determinationin the right tibia of rabbits were graded,white blood cell count(WBC)and erythrocyte sedimentation rate(ESR)were measured and interleukin(IL)-1β,IL-4,IL-6 and IL-10 levels in sera were determined by ELISA on the 2nd,4th and 6th week after treatment.Results:For Guyan prescription groups(3.6 g/kg on the 2nd,4th and 6th week and 1.8 g/kg on the 4th and 6th week,respectively),the X-ray grades were significantly improved as compared with the model group(P<0.05).As compared with the model group,WBC and ESR in Guyan prescription groups were markedly decreased on the 2nd,4th and 6th week respectively(P<0.05).The serum levels of proinflammatory cytokines(IL-1β and IL-6)in Guyan prescription groups(3.6 and 1.8 g/kg)were significantly reduced at the 2nd,4th and 6th week respectively(P<0.05),while anti-inflammatory cytokine(IL-4)was significantly enhanced on the 6th week(P<0.01).Besides,the serum levels of anti-inflammatory cytokine(IL-10)in Guyan prescription high dose group was markedly increased at the 4th and 6th week respectively(P<0.05).Conclusion:The mechanism of Guyan prescription against chronic osteomyelitis in rabbits may be related to the inhibition of pathogenic bacteria,the control of inflammatory reaction and the restoration of proinflammatory/anti-inflammatory cytokine balance.

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备注/Memo

备注/Memo:
(收稿日期:2020-04-13) △通信作者 E-mail:158130707@qq.com
更新日期/Last Update: 2020-09-10