[1]刘凡杰 殷颖 安建鹏 王丹丹 甘东浩 师彬△.网络药理学视角下杜仲抗骨质疏松的作用机制研究[J].中国中医骨伤科杂志,2020,28(01):43-48.
　LIU Fanjie YIN Ying AN Jianpeng WANG Dandan GAN Donghao SHI Bin.Anti-osteoporosis Mechanism of Eucommia Ulmoides from the Perspective of Network Pharmacology[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2020,28(01):43-48.
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网络药理学视角下杜仲抗骨质疏松的作用机制研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第28卷
期数:
2020年01期
页码:
43-48
栏目:
文献研究
出版日期:
2020-01-15

文章信息/Info

Title:
Anti-osteoporosis Mechanism of Eucommia Ulmoides from the Perspective of Network Pharmacology
文章编号:
1005-0205(2020)01-0043-06
作者:
刘凡杰1 殷颖2 安建鹏1 王丹丹1 甘东浩3 师彬1△
1山东省罕少见病重点实验室,山东省医学科学院颈肩腰腿 痛医院,山东省医药生物技术研究中心,山东第一医科大学 (山东省医学科学院)(济南,250012) 2山东中医药大学附属医院 3山东中医药大学
Author(s):
LIU Fanjie1 YIN Ying2 AN Jianpeng1 WANG Dandan1 GAN Donghao3 SHI Bin1△
1Key Laboratory for Rare & Uncommon Diseases of Shandong Province,Back and Neck Pain Hospital,Shandong Medicinal Biotechnology Center,Shandong First Medical University & Shandong Academy of Medical Sciences,Jinan 250012,China; 2Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,China; 3Shandong University of Traditional Chinese Medicine,Jinan 250355,China.
关键词:
杜仲;  骨质疏松;  网络药理学;  作用机制
Keywords:
Eucommia ulmoides;  osteoporosis;  network pharmacology;  mechanism
分类号:
R274.39
文献标志码:
A
摘要:
目的:基于网络药理学方法,根据杜仲活性化合物结构预测其作用靶点,与骨质疏松(OP)相关治疗靶点进行扩展后映射,拓扑出Hub节点并进行富集分析,揭示杜仲抗OP的物质基础及作用机制。方法:通过中药系统药理学数据库和分析平台(TCMSP),根据药代动力学特征筛选出杜仲的主要活性成分,并使用有机小分子生物活性数据库(PubChem)和Swiss Target Prediction数据库,根据化合物结构相似性预测出可能作用靶点,然后通过Drugbank搜集目前OP治疗药物及其相关靶点,结合预测靶点分别进行扩展后取交集,将所得重合靶点借助CytoNCA软件,根据相关节点参数拓扑出相互作用的关键节点,再导入String构建蛋白质相互作用网络图,最后通过DAVID数据库对关键节点进行生物功能及代谢通路分析。结果:筛选出15个杜仲活性成分,根据靶点预测技术预测出相关靶点196个; 检索出OP相关靶点43个,经扩展-取交集-拓扑分析后共得152个Hub节点,根据String数据库构建蛋白相互作用网络,富集分析显示杜仲可能通过多条主流信号通路及细胞代谢全过程对OP产生调控作用。结论:杜仲可能通过直接或间接作用于靶点,参与调控上下游多条信号通路以干预OP的进展,为揭示其抗OP的机制及后续研究奠定了基础。
Abstract:
Objective:Based on the network pharmacology method,the target was predicted according to the active compound structure of Eucommia ulmoides,and the therapeutic targets related to osteoporosis(OP)were expanded and intersected with those.The Hub node was topologically analyzed and enriched to reveal the material basis of anti-OP of Eucommia ulmoides.Methods:The main active ingredients of Eucommia ulmoides was screened according to the pharmacokinetic characteristics by traditional Chinese medicine database and analysis platform system pharmacology(TCMSP),and or-ganic small molecule biological active database(PubChem)and Swiss Target Prediction database was used to predict possible targets according to the compound structure similarity.Current OP therapeutic drugs and its related targets were collected by means of the Drugbank,and then combine the predicted targets to expand respectively to take the intersection.The Hub nodes were extracted from the obtained coincident targets of the interaction according to the relevant node parameters by CytoNCA software and imported into String to construct the protein interaction network diagram.Finally the DAVID database was used to perform analysis of biological functions and metabolic pathways of Hub nodes.Results:15 active components of Eucommia ulmoides were selected and 196 targets were predicted according to the target prediction technology.43 OP-related targets were retrieved,and 152 Hub nodes were obtained after extension-intersection and topological analysis.Protein interaction network was constructed according to String database.Enrichment analysis showed that Eucommia ulmogenes may exert regulatory effects on OP through multiple mainstream signal pathways and the whole process of cell metabolism.Conclusion:Based on the network pharmacology research,it is suggested that Eucommia ulmoides may be involved in the regulation of multiple signaling pathways in the upstream and downstream to interfere with the progress of OP through direct or indirect targets,which lays a foundation for revealing and further studying the anti-OP mechanism.

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备注/Memo

备注/Memo:
基金项目:泰山学者特聘专家计划(ts201511074) 山东省自然科学基金面上项目(ZR2019MH134) 山东省重点研发计划项目(2016GSF202042) 山东第一医科大学学术提升计划(2019QL003) △通信作者 E-mail:sdyky-shibin@163.com(收稿日期:2019-08-01)
更新日期/Last Update: 2020-01-15