[1]劳杨骏 裘一丹 徐彬 盛红枫 张树良 刘亦杨 沈立锋 吴承亮.轴向负载诱导肾虚小鼠椎间盘退变的实验研究[J].中国中医骨伤科杂志,2020,28(01):13-17.
　LAO Yangjun QIU Yidan XU Bin SHENG Hongfeng ZHANG Shuliang LIU Yiyang SHEN Lifeng WU Chengliang.Experimental Study on the Intervertebral Disc Degeneration Induced by Spinal Axial Loading in Mice with Kidney Deficiency[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2020,28(01):13-17.
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轴向负载诱导肾虚小鼠椎间盘退变的实验研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第28卷
期数:
2020年01期
页码:
13-17
栏目:
实验研究
出版日期:
2020-01-15

文章信息/Info

Title:
Experimental Study on the Intervertebral Disc Degeneration Induced by Spinal Axial Loading in Mice with Kidney Deficiency
文章编号:
1005-0205(2020)01-0013-05
作者:
劳杨骏1 裘一丹2 徐彬1 盛红枫1 张树良1 刘亦杨1 沈立锋1 吴承亮2
1浙江省立同德医院(杭州,310012) 2浙江中医药大学
Author(s):
LAO Yangjun1 QIU Yidan2 XU Bin1 SHENG Hongfeng1 ZHANG Shuliang1 LIU Yiyang1 SHEN Lifeng1 WU Chengliang2
1Tongde Hospital of Zhejiang Province,Hangzhou 310012,China; 2Zhejiang Chinese Medical University,Hangzhou 310053,China.
关键词:
肾虚;  椎间盘退变;  热板鼠笼;  轴向力学负载
Keywords:
kidney deficiency;  intervertebral disc degeneration;  hot plate cage;  spinal axial loading
分类号:
R-33
文献标志码:
A
摘要:
目的:探究采用自制的热板鼠笼诱导的间断轴向力学负载环境对肾虚小鼠腰椎间盘退变的影响。方法:选择16个月龄老年小鼠36只,随机分为对照组、模型组及肾虚模型组,每组12只。每日于固定时间段将肾虚模型组及模型组小鼠放入自制的热板鼠笼,开始跳跃后计时10 min,对照组小鼠不予处理。分别在造模1个月和3个月后,通过HE、ABH染色分别比较三组小鼠腰椎间盘组织学差异,通过免疫组化测定Ⅱ型胶原来说明三组小鼠椎间盘退变的不同情况。结果:染色结果表明模型组和肾虚模型组小鼠椎间盘高度明显小于对照组,模型组与肾虚模型组样本软骨终板都存在更严重的骨化,模型组较对照组明显。模型组与肾虚模型组椎间盘内软骨老化较对照组明显升高,且肾虚模型组髓核的软骨细胞退变作用更为严重,其阳性染色较模型组有明显的升高。结论:间断的轴向力学负载能够促进小鼠椎间盘内软骨细胞退变和基质分解酶的表达,从而诱导小鼠腰椎间盘退变的发生和发展,且其对肾虚小鼠腰椎间盘能造成更为严重的椎间盘退变。
Abstract:
Objective:To investigate the efficacy of intermittent spinal axial mechanical loading induced by self-made hot plate cage on lumbar intervertebral disc degeneration in mice with kidney deficiency.Methods:Thirty-six 16 months-old mice were randomly divided into kidney deficiency model group,model group and control group with 12 mice in each group.The mice of kidney deficiency model group and model group were put into the self-made hot-plate cage at the same time every day,the control group was not treated.Histological differences of lumbar intervertebral disc in three groups were compared by HE and ABH staining,and the type Ⅱ Collagen was detected by immunohistochemistry to explain the different conditions of intervertebral disc degeneration in the three groups of mice after modeling for 1 month and 3 months respectively.Results:The results of staining showed that the height of intervertebral disc in model group and kidney deficiency model group was significantly lower than that in control group.The cartilage endplates in the model group and the kidney deficiency model group were more ossified than those in the control group.The aging of intervertebral disc cartilage in model group and kidney-deficiency model group was higher than that in control group,and the degeneration of nucleus pulposus in kidney-deficiency model group was more serious,and the positive staining was higher than that in model group.Conclusion:Intermittent spinal axial mechanical loading can promote the degeneration of chondrocyte and increase expression of decomposing enzyme in intervertebral disc of mice,which induce the initiation and development of lumbar disc degeneration in mice.And it can cause more serious intervertebral disc degeneration in kidney deficiency mice.

参考文献/References:

[1] 劳杨骏,徐涛涛,杨笑奇,等.小鼠椎间盘退变模型的构建策略[J].中国中医急症,2015,24(10):1783-1786.
[2] 余城墙,张宇,谢程欣,等.椎间盘退变分子生物学机制及再生治疗的优势与未来[J].中国组织工程研究,2019,23(30):4889-4896.
[3] SHOWALTER B L,BECKSTEIN J C,MARTIN J T,et al.Comparison of animal discs used in disc research to human lumbar disc:torsion mechanics and collagen content[J].Spine(Phila Pa 1976),2012,37(15):900-907.
[4] 张驰,吕浩源,章晓云,等.长非编码RNA在椎间盘退变中的机制[J].中国组织工程研究,2019,23(27):4280-4285.
[5] SAKAI D,NISHIMURA K,TANAKA M,et al.Migration of bone marrow-derived cells for endogenous repair in a new tail-looping disc degeneration model in the mouse:a pilot study[J].Spine J,2015,15(6):1356-1365.
[6] PAPUGA M O,KWOK E,YOU Z,et al.TNF is required for the induction but not the maintenance of compression-induced BME signals in murine tail vertebrae:limitations of anti-TNF therapy for degenerative disc disease[J].J Orthop Res,2011,29(9):1367-1374.
[7] HSIEH A H,LOTZ J C.Prolonged spinal loading induces matrix metalloproteinase-2 activation in intervertebral discs[J].Spine(Phila Pa 1976),2003,28(16):1781-1788.
[8] LIU L T,HUANG B,LI C Q,et al.Characteristics of stem cells derived from the degenerated human intervertebral disc cartilage endplate[J].PloS One,2011,6(10):e26285.
[9] CALVO-ECHENIQUE A,CEGONINO J,CORREA-MARTIN L,et al.Intervertebral disc degeneration:an experimental and numerical study using a rabbit model[J].Med Biol Eng Comput,2018,56(5):865-877.
[10] TAM V,CHAN W C W,LEUNG V Y L,et al.Histological and reference system for the analysis of mouse intervertebral disc[J].J Orthop Res,2018,36(1):233-243.
[11] 劳杨骏,裘一丹,徐彬,等.轴向负载诱导小鼠椎间盘退变模型的建立[J].中医正骨,2019,31(5):1-6.
[12] ZHANG J,LI Z,CHEN F,et al.TGF-beta1 suppresses CCL3/4 expression through the ERK signaling pathway and inhibits intervertebral disc degeneration and inflammation-related pain in a rat model[J].Exp Mol Med,2017,49(9):e379.
[13] SAKAI D,ANDERSSON G B.Stem cell therapy for intervertebral disc regeneration:obstacles and solutions[J].Nat Rev Rheumatol,2015,11(4):243-256.
[14] 冯帅华,吴官保,杨届,等.去卵巢致大鼠腰椎间盘退变模型初探[J].中国医学创新,2015,12(15):19-22.
[15] 李震,张柳,穆树林,等.仙灵骨葆预防卵巢切除大鼠的腰椎间盘退变[J].中国组织工程研究与临床康复,2010,14(15):2722-2726.
[16] 姚长风,赵永见,牛凯,等.去卵巢致肾虚的腰椎间盘退变模型的实验研究[J].中国骨伤,2013,26(12):1015-1022.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81573994) 国家自然科学基金资助项目(81804113) 浙江省中医药科技计划项目(2017ZA017) 浙江省基础公益研究计划项目(LGF19H60007)(收稿日期:2019-07-03)
更新日期/Last Update: 2020-01-15