[1]高伟华,李玉杰,杨鸿冉,等.加味阳和汤对不同时期膝骨关节炎兔关节软骨结构的影响[J].中国中医骨伤科杂志,2019,27(05):7-13.
　GAO Weihua,LI Yujie,YANG Hongran,et al.Study on the Effect of Jiawei Yanghe Decoction on Articular Cartilage Structure of Rabbits with Knee Osteoarthritis at Different Stages[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2019,27(05):7-13.
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加味阳和汤对不同时期膝骨关节炎兔关节软骨结构的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第27卷
期数:
2019年05期
页码:
7-13
栏目:
实验研究
出版日期:
2019-05-15

文章信息/Info

Title:
Study on the Effect of Jiawei Yanghe Decoction on Articular Cartilage Structure of Rabbits with Knee Osteoarthritis at Different Stages
文章编号:
1005-0205(2019)05-0007-07
作者:
高伟华1; 李玉杰1; 杨鸿冉1; 李志超1; 齐鲁1; 刘文斌1; 王文波1△; 董建文1△
1山东中医药大学第二附属医院骨科(济南,250001)
Author(s):
GAO Weihua1; LI Yujie1; YANG Hongran1; LI Zhichao1; QI Lu1;  LIU Wenbin1; WANG Wenbo1△; DOGN Jianwen1△
1Orthopedics Department,the Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250001, China.
关键词:
膝骨关节炎;  软骨;  加味阳和汤;  动物实验研究
Keywords:
knee osteoarthritis;  articular cartilage;  Jiawei Yanghe decoction;  animal experimental study
分类号:
R-33
文献标志码:
A
摘要:
目的:观察加味阳和汤对膝骨关节炎兔关节软骨结构的影响。方法:将60只健康新西兰大白兔随机分为正常组、模型组和实验组。除正常组外,其余两组动物均按照改良Hulth法进行膝骨关节炎造模后,分组处理,4,6及8周后取材,制作标本,采用X线、光学显微镜、透射电镜等观察关节软骨中超微结构的变化。结果:在干预的4,6及8周的不同时间内,模型组和实验组均可见膝关节滑膜增生,内侧间隙变窄,并见增生骨赘,同一时间段内实验组程度较模型组轻; 两组均出现关节软骨光滑度降低、软骨细胞排列紊乱、软骨细胞数量减少、粗面内质网数量减少、胶原蛋白的排列紊乱及细胞核膜部分破裂等变化,同一时间段内模型组表现更重,同一组内骨性关节炎时间越久,则表现越重。结论:加味阳和汤可抑制不同时间段内兔膝骨关节炎关节软骨的退变,减轻软骨细胞的退变、减轻内质网的退变、延缓胶原蛋白的紊乱,这可能是其治疗膝骨关节炎的作用机制之一。
Abstract:
Objective:To observe the impact of Jiawei Yanghe decoction on the osteoarthritis knee cartilage of rabbit. Methods:60 healthy New Zealand white rabbits were randomly divided into three groups, normal group,control group and the experimental group. In addition to the normal group, the others were given modified Hulth method of modeling, driving to get early osteoarthritis model for 4,6 and 8 weeks, observing the changes on the microstructure according to modeling,X-ray,light microscope,transmission electron microscope. Results:In the interfering weeks of 4,6 or 8, there were some different changes of different kinds of symptoms in both model group and experimental group. Conclusion: Jiawei Yanghe decoction delay degeneration osteoarthritis knee, of the rabbit and effectively improve the rabbit knee function, and delay cartilage degeneration and alleviate the degeneration of chondrocyte and endoplasmic reticulum at different times.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(8150150763,81173286)山东省中医药科技发展计划(2013ZDZK-069)济南市科技发展计划项目(201102063)△通信作者 E-mail:董建文 dongjianwen2006@sina.com王文波 wangvenbo@126.com
更新日期/Last Update: 2019-05-15