[1]刁志君,姜宏,刘锦涛△,等.益气活血方介导促炎因子促进破裂型腰椎间盘突出后重吸收的机制研究[J].中国中医骨伤科杂志,2019,27(05):1-6.
　DIAO Zhijun,JIANG Hong,LIU Jintao,et al.Mechanism of Yiqi Huoxue Recipe in Promoting Spontaneous Resorption of Ruptured Lumbar Disc Herniation[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2019,27(05):1-6.
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益气活血方介导促炎因子促进破裂型腰椎间盘突出后重吸收的机制研究()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第27卷
期数:
2019年05期
页码:
1-6
栏目:
实验研究
出版日期:
2019-05-15

文章信息/Info

Title:
Mechanism of Yiqi Huoxue Recipe in Promoting Spontaneous Resorption of Ruptured Lumbar Disc Herniation
文章编号:
1005-0205(2019)05-0001-06
作者:
刁志君1; 2; 姜宏2; 刘锦涛2△; 刘建文3; 李月琪3; 李红卫2; 李晓春2
1上海中医药大学(上海,201203) 2南京中医药大学附属苏州医院 3华东理工大学
Author(s):
DIAO Zhijun1; 2; JIANG Hong2; LIU Jintao2△; LIU Jianwen3; LI Yueqi3; LI Hongwei2; LI Xiaochun2
1Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China; 2Suzhou Hospital,Nanjing University of Traditional Chinese Medicine,Suzhou 215009,Jiangsu China; 3East China University of Science and Technology,Shanghai 200237,China.
关键词:
益气活血方;  破裂型;  腰椎间盘突出;  重吸收
Keywords:
Yiqi Huoxue recipe;  ruptured;  lumbar disc herniation;  spontaneous resorption
分类号:
R-33
文献标志码:
A
摘要:
目的:通过比较益气活血方介导对趋化因子5(CCL5)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)在破裂及非破裂型髓核细胞中表达的影响,探索益气活血方促进破裂型腰椎间盘突出后重吸收的机制。方法:收集破裂型与非破裂型腰椎间盘突出症患者的髓核组织各6例,采用酶消法分离细胞,放入含20%胎牛血清的DMEM/F12培养基中培养,定期用倒置相差显微镜观察细胞贴壁及生长情况。用10只3个月龄SD大鼠制备含药(益气活血方)血清及无药(生理盐水)血清,用两种血清分别培养破裂型及非破裂髓核细胞。提取两种血清体外给药后的破裂及非破裂原代髓核细胞RNA。利用实时荧光定量PCR(实时qPCR)技术分别检测体外含药血清给药培养后及非含药血清培养后的破裂及非破裂型髓核细胞中CCL5,TNF-α,IL-1β mRNA的表达,记录统计分析数据。提取含药血清及非含药血清体外给药培养后的破裂及非破裂型原代髓核细胞蛋白,利用免疫蛋白印迹(Western Blotting)技术分别检测两种血清体外给药培养后的破裂及非破裂型原代髓核细胞中CCL5,TNF-α及IL-1β蛋白的表达,记录统计分析数据。结果:CCL5,TNF-α及IL-1β在破裂型髓核中的表达明显高于非破裂型(P<0.05),在破裂型含药组髓核细胞中的表达显著高于无药组(P<0.05)。结论:益气活血方可提高促炎因子在破裂型髓核细胞中高表达,有利于髓核细胞的炎性反应,加速细胞外基质(ECM)的降解,从而有利于重吸收现象的发生,且破裂型髓核比非破裂型髓核更容易发生重吸收现象。
Abstract:
Objective:Investigating the mechanism of Yiqi Huoxue recipe in promoting resorption of ruptured lumbar disc herniation, by comparing the expression of CCL5, TNF-α, IL-1β in ruptured and unruptured nucleus pulposus cells mediated by Yiqi Huoxue recipe.Methods:Collecting nucleus pulposus of 6 patients with ruptured lumbar disc herniation and 6 patients with unruptured lumbar disc herniation, separationing of cells by enzymatic elimination, primarying culture in DMEM/F12 medium containing 20% fetal bovine serum, observing cell attachment and growth regular by inverted phase contrast microscope. Ten three-month-old SD rats were prepared for the preparation of drug-containing serum and drug-free serum, cultured ruptured and unruptured nucleus pulposus cells by these serum. Extraction of ruptured and unruptured primary nucleus pulposus cells RNA used two kinds of serum. Detecting the expression of CCL5, TNF-α, IL-1β mRNA in ruptured and unruptured primary nucleus pulposus cells RNA used two kinds of serum by real-time qPCR technology, recording statistical analysis data.Extraction of ruptured and unruptured primary nucleus pulposus cells protein used two kinds of serum, detecting the expression of CCL5, TNF-α, IL-1β mRNA in ruptured and unruptured primary nucleus pulposus cells RNA used two kinds of serum by Western Blotting technology, recording statistical analysis data. Results:The expression of CCL5, TNF-α and IL-1β in ruptured nucleus pulposus was significantly higher than that in unruptured(P<0.05). The expression in the ruptured nucleus pulposus cells of the drug-containing group was significantly higher than that in the drug-free group(P<0.05).Conclusion:Yiqi Huoxue Recipe can increase the expression of pro-inflammatory factors in ruptured nucleus pulposus cells, is useful for inflammatory response of nucleus pulposus cells,can accelerate the degradation of extracellular matrix. Thereby facilitating the occurrence of resorption.The ruptured nucleus pulposus is more prone to resorption than the unruptured nucleus pulposus.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(81473691)△通信作者 E-mail:okdoctor@163.com
更新日期/Last Update: 2019-05-15