[1]宋奕 朱寅△ 丁道芳.丹参酮ⅡA对软骨细胞Ⅱ型胶原及Wnt/β-catenin信号通路的影响[J].中国中医骨伤科杂志,2018,26(09):1-4.
　SONG Yi ZHU Yin DING Daofang.Effect of Tanshinone ⅡA on Collagen Ⅱ and Wnt/β-catenin Signal Pathway of Rat Chondrocytes[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2018,26(09):1-4.
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丹参酮ⅡA对软骨细胞Ⅱ型胶原及Wnt/β-catenin信号通路的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第26卷
期数:
2018年09期
页码:
1-4
栏目:
实验研究
出版日期:
2018-09-06

文章信息/Info

Title:
Effect of Tanshinone ⅡA on Collagen Ⅱ and Wnt/β-catenin Signal Pathway of Rat Chondrocytes
文章编号:
1005-0205(2018)09-0001-04
作者:
宋奕1 朱寅1△ 丁道芳2; 3
1苏州市中西医结合医院(江苏 苏州,215000) 2上海中医药大学附属曙光医院石氏伤科医学中心 3上海市中医药研究院骨伤科研究所
Author(s):
SONG Yi1 ZHU Yin1△ DING Daofang2; 3
1Suzhou Hospital of Integrated Chinese and Western,Suzhou 215000,Jiangsu China; 2Shi's Center of Orthopedics and Traumatology, Shuguang Hospital Affiliated to Shanghai University of TCM,Shanghai 201203,China; 3Institute of Traumatology and Orthopedics, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203.
关键词:
丹参酮ⅡA;  软骨细胞;  Ⅱ型胶原;  Wnt/β-catenin 信号通路
Keywords:
Keywords:tanshinone ⅡA;  chondrocytes;  collagen Ⅱ;  Wnt/β-catenin signal pathway
分类号:
R-33
文献标志码:
A
摘要:
目的:探讨不同浓度丹参酮ⅡA对软骨细胞Ⅱ型胶原及Wnt/β-catenin信号通路的影响。方法:提取新生SD大鼠原代软骨细胞,并通过Ⅱ型胶原蛋白免疫荧光进行鉴定,取P1代细胞进行实验,共设5组,其中1组设为空白对照组,另外4组分别用6.25 μmol/L,12.5 μmol/L,25 μmol/L及50 μmol/L浓度丹参酮ⅡA对其进行干预,24 h后通过蛋白印迹分析法(Western Blot)检测软骨细胞Ⅱ型胶原及β-catenin蛋白的表达情况。结果:随着丹参酮ⅡA浓度的增加,Ⅱ型胶原蛋白表达增加,12.5 μmol/L,25 μmol/L及50 μmol/L丹参酮ⅡA组Ⅱ型胶原蛋白量均较对照组增高(P<0.05),且Ⅱ型胶原蛋白量与丹参酮ⅡA浓度呈正相关(r=0.949,P<0.001),各丹参酮ⅡA组β-catenin蛋白量均较对照组降低(P<0.05),且β-catenin蛋白量与丹参酮ⅡA浓度呈负相关(r=-0.949,P<0.001)。结论:丹参酮ⅡA可抑制Wnt/β-catenin信号通路,促进软骨细胞Ⅱ型胶原表达,延缓软骨细胞退变。
Abstract:
Abstract Objective:To investigate the effect of Tanshinone ⅡA(TAN ⅡA)on collagen Ⅱ and Wnt/β-catenin signal pathway of rat chondrocytes in vitro. Methods: SD rat articular chondrocytes were isolated, and immunofluorescence was performed to identify the cells. A total of 5 groups were set up, 1 of which was a blank control group, and the other 4 groups were treated with different concentrations of TAN ⅡA(6.25 μmol/L, 12.5 μmol/L, 25 μmol/L and 50 μmol/L). The expression of β-catenin and collagen Ⅱ were examined by Western-Blot after 24 h.Results: The groups of 12.5 μmol/L, 25 μmol/L, 50 μmol/L concentration of TAN ⅡA expressed higher collagen Ⅱ than the control group(P<0.05). The expression of collagen Ⅱ was increased as the concentration of TAN ⅡA had a rising trend(r=0.949, P<0.001). All the groups treated with TAN ⅡA expressed lower β-catenin than the control group(P<0.05). The expression of β-catenin was decreased as the concentration of TAN ⅡA had a rising trend(r=-0.949,P<0.001). Conclusion: TAN ⅡA can inhibit the Wnt/β-catenin signaling pathway, promote the expression of type Ⅱ collagen in chondrocytes, and delay the degeneration of chondrocytes.

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备注/Memo

备注/Memo:
基金项目:苏州市科技局指导性项目(SYSD2016051, SYSD2016050) △通信作者 E-mail:395344510@qq.com
更新日期/Last Update: 2018-09-06