[1]贾庆运 王拥军 梁倩倩 施杞△.防己黄芪汤对胶原诱导性关节炎小鼠的影响[J].中国中医骨伤科杂志,2018,26(05):6-9.
　JIA Qingyun WANG Yongjun LIANG Qianqian SHI Qi.Effect of Fangji Huangqi Decoction on Arthritis Mice Induced by Collagen[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2018,26(05):6-9.
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防己黄芪汤对胶原诱导性关节炎小鼠的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第26卷
期数:
2018年05期
页码:
6-9
栏目:
实验研究
出版日期:
2018-05-02

文章信息/Info

Title:
Effect of Fangji Huangqi Decoction on Arthritis Mice Induced by Collagen
文章编号:
1005-0205(2018)05-0006-04
作者:
贾庆运1; 2; 3; 4 王拥军1; 2; 4; 5 梁倩倩1; 2; 4 施杞1; 2; 4△
1上海中医药大学附属龙华医院(上海,200032) 2上海中医药大学脊柱病研究所 3山东省日照市中医医院 4上海筋骨理论与治法教育部重点实验室 5上海中医药大学康复医学院
Author(s):
JIA Qingyun1; 2; 3; 4 WANG Yongjun1; 2; 4; 5 LIANG Qianqian1; 2; 4 SHI Qi1; 2; 4 △
1Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China; 2Institute of Spine,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China; ···
关键词:
防己黄芪汤;  类风湿关节炎;  CIA小鼠;  关节炎指数
Keywords:
Keywords: Fangji Huangqi decoction;  rheumatoid arthritis;  collagen induced arthritis mice;  arthritis index
分类号:
R-33
文献标志码:
A
摘要:
目的:观察防己黄芪汤对Ⅱ型胶原诱导性关节炎(CIA)小鼠的影响。方法:6～7周龄的DBA/1J小鼠适应性喂养1周后随机分为4组造模,对照组、模型组、甲氨喋呤组、防己黄芪汤组各10只小鼠。除对照组外,其余小鼠给予牛属Ⅱ型胶原+完全弗氏佐剂进行CIA小鼠第一次免疫,第21天给予牛属Ⅱ型胶原+不完全弗氏佐剂进行加强免疫,次日各组开始给药20 d,每5 d观察小鼠体质量、关节炎指数、足掌厚度、受累肢体数目。结果:与模型组相比,防己黄芪汤组和甲氨蝶呤组对CIA小鼠关节炎指数、足掌肿胀有显著改善作用,差异有统计学意义(P<0.05); 对CIA小鼠体质量及受累肢体数目的影响,差异无统计学意义(P>0.05)。结论:防己黄芪汤对CIA小鼠的关节炎症和肿胀有显著改善作用,是治疗类风湿性关节炎的潜在有效方剂。
Abstract:
Abstracts Objective: To explore the effect of Fangji Huangqi decoction(FJHQD)on arthritis mice induced by type Ⅱ collagen.Methods: Six to seven weeks old DBA/1J mice were fed for one week,and then they were randomly divided into four groups:normal control group,model group,methotrexate group,and FJHQD group,10 mice in each group.The model of collagen induced arthritis(CIA)was established except mice in the normal control group,by the injection of Bovine collagen type Ⅱ and complete Freund's adjuvant for the first immunization.On the day 21th,the mice were received the booster injection by Bovine collagen type Ⅱ and incomplete Freund's adjuvant,and the next day the mice were received administration of corresponding medicines once daily for 20 days.The mice body weight,arthritis index,foot thickness and number of involved limbs were observed every 5 days after treatment.Results: Compared with CIA model group,the FJHQD group and methotrexate group have significant improvement on the arthritis index and foot thickness,there were no difference between the weight and the number of involved limbs.Conclusion: FJHQD is effective for improving the inflammation and swelling of the joints of the CIA mice,which is an potential effective prescription used for treating rheumatoid arthritis.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金重大国际合作项目(81220108027) 国家自然科学基金重点项目(81330085) △通信作者 E-mail:shiqi@hotmail.com
更新日期/Last Update: 2018-05-02