[1]周逸敏,邱友利,张俐△.膝骨关节炎合并骨质疏松症大鼠模型的建立与评估[J].中国中医骨伤科杂志,2017,25(09):1-4.
　ZHOU,Yimin,QIU,et al.Establishment and Evaluation of Rat Model of Osteoarthritis and Osteoporosis of Knee Joint[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2017,25(09):1-4.
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膝骨关节炎合并骨质疏松症大鼠模型的建立与评估()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第25卷
期数:
2017年09期
页码:
1-4
栏目:
实验研究
出版日期:
2017-09-08

文章信息/Info

Title:
Establishment and Evaluation of Rat Model of Osteoarthritis and Osteoporosis of Knee Joint
文章编号:
1005-0205(2017)09-0001-04
作者:
周逸敏1; 邱友利1; 张俐1; 2△
1福建中医药大学(福州,350122) 2厦门医学院
Author(s):
ZHOU; Yimin1; QIU; Youli1; ZHANG; Li1; 2△
1Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China; 2Xiamen Medical School,Xiamen 361008,Fujian China.
关键词:
膝骨关节炎;  骨质疏松症;  动物模型;  实验研究
Keywords:
knee osteoarthritis;  osteoporosis;  animal model;  experimental study
分类号:
R-33
文献标志码:
A
摘要:
目的:模拟临床中老年女性绝经后合并骨关节炎的病理生理特点,建立膝骨关节炎合并骨质疏松症大鼠动物模型。方法:采用经典去卵巢法构建骨质疏松症模型后的大鼠加疲劳性跑台训练。18只6月龄雌性SD大鼠,采用抽签法随机均分为空白对照组(正常组)、单纯去卵巢组(骨质疏松组)和膝骨关节炎合并骨质疏松组(模型组),每组6只。单纯去卵巢组和模型组在无菌条件下摘除双侧卵巢后,单纯去卵巢组常规喂养,模型组术后恢复1周后行跑台疲劳性训练4周,空白组不作任何处理。5周后取右侧股骨、左侧膝关节、血清及尿液行组织学、力学及免疫学分析。结果:与空白组比较,单纯去卵巢组与模型组血清雌二醇浓度显著降低(P<0.01),最大载荷、断裂载荷降低(P<0.05); 与单纯去卵巢组比较,模型组大鼠尿液CTX-Ⅱ浓度明显升高(P<0.01),模型组大鼠关节软骨呈现轻度软骨损伤。结论:采用经典去卵巢法能构建骨质疏松症模型,并在此基础上使用动物跑台进行疲劳性运动可以造成膝关节劳损,从而成功建立符合女性中老年膝骨关节炎合并骨质疏松症的新模型。
Abstract:
Abstract Objective:Simulating the clinical older postmenopausal women combined osteoarthritis pathological physiology characteristic,to attempt to establish KOA+OP animal model.Methods:After the classic bilateral ovariectomy method to build osteoporosis model rats,adding the fatigue treadmill training.Using the lottery method randomly divided the eighteen 6-month female SD rats into blank group(normal group),ovariectomed group(osteoporosis group)and knee osteoarthritis with osteoporosis group(model group),6 rats as a group.Ovariectomed group and model group under aseptic conditions after excision bilateral ovaries,regular feeding ovariectomed group,the model group postoperative recovery 1 week,fatigue treadmill training 4 weeks,don't make any deal with blank group.After 4 weeks of administration,take on the right side of the femur,left side of the knee joint,serum and urine was sampled to experiment histology,mechanics and immunological analysis.Results:Compared with blank group,ovariectomed group and model group had a significantly lower E2(P<0.01),the maximum load,fracture load reduced(P<0.05); Compared with ovariectomed group,model group rats urine CTX-Ⅱ concentration increased significantly(P<0.01),the model group rats present mild cartilage injury of articular cartilage.Conclusion:Using the method of classic to ovarian can build osteoporosis model,and on this basis,the use of animals run movement can cause knee strain fatigue line,thus successfully established in conformity with the female senile knee osteoarthritis with a new model of osteoporosis.

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备注/Memo

备注/Memo:
基金项目:福建省自然科学基金项目(2016J01380) 福建省百千万领军人才 1福建中医药大学(福州,350122) 2厦门医学院 △通信作者 E-mail:zhanglil626@163.com
更新日期/Last Update: 1900-01-01