[1]刘卓,李益亮,张志,等.桃红四物汤对兔皮片移植模型Notch4受体表达的影响[J].中国中医骨伤科杂志,2017,25(05):1-4.
　LIU Zhuo LI Yiliang ZHANG Zhi FENG Haibo ZENG Xiangjing SUN Shaoqiu.Effect of Taohongsiwu Decoction on Notch4 Receptor Expression in the Rabbit Skin Grafting Model[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2017,25(05):1-4.
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桃红四物汤对兔皮片移植模型Notch4受体表达的影响()
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第25卷
期数:
2017年05期
页码:
1-4
栏目:
实验研究
出版日期:
2017-05-08

文章信息/Info

Title:
Effect of Taohongsiwu Decoction on Notch4 Receptor Expression in the Rabbit Skin Grafting Model
文章编号:
1005-0205(2017)05-0001-04
作者:
刘卓1;  李益亮2;  张志1;  冯海波2;  曾祥晶2;  孙绍裘2△
1.湖南中医药大学(长沙,410208)
2.湖南中医药大学第二附属医院
△.通信作者 E-mail:54sunshaoqiu@163.com
Author(s):
LIU Zhuo1 LI Yiliang2 ZHANG Zhi1 FENG Haibo2 ZENG Xiangjing2 SUN Shaoqiu2△
1.Hunan University of Traditional Chinese Medicine,Changsha 410208,China;
2.The Second Hospital,Affiliated to Hunan University of Traditional Chinese Medicine,Changsha 410005,China.
关键词:
桃红四物汤;  Notch信号通路;  Notch4受体;  血管新生
Keywords:
taohongsiwu decoction;  notch signaling pathway;  notch4 receptor;  angiogenesis
分类号:
R-33
文献标志码:
A
摘要:
目的:观察桃红四物汤对兔自体皮片移植模型Notch4受体表达的影响。方法:将60只新西兰大白兔随机分为4组,即桃红四物汤组、阻断剂组、模型组、假手术组,每组15只。造模后分组干预,并于术后第2,5,8天三个时限,从每组随机选取5只兔,行空气栓塞处死,切取移植皮片吻合区皮片组织,苏木精-伊红染色后在光镜下计算血管密度,采用免疫组化法测定Notch4受体蛋白表达情况。结果:桃红四物汤组移植区皮片的新生血管密度明显高于模型组、假手术组及阻断剂组(P<0.05),差异有统计学意义。Notch4受体蛋白表达含量桃红四物汤组表达量高于模型组、假手术组及阻断剂组(P<0.05),差异有统计学意义。结论:桃红四物汤能上调组织中血管新生相关因子Notch4受体的表达,促进移植皮片成活。
Abstract:
Objective:To observe the effect of Taohongsiwu decoction on Notch4 receptor expression in the rabbit autologous skin grafting model.Methods:All 60 New Zealand white rabbits were randomly divided into four groups:the Taohongsiwu decoction group,the blocker group,the model group and the sham operation group,15 rabbits in each group.Group interventions were taken after modeling,and five rabbits were randomly selected from each group at 2nd,5th and 8th day after operation.The rabbits were sacrificed by air embolization,and the anastomosed area skin tissues of the skin grafts were cut.The vascular density was calculated under light microscope after HE staining,the expression of Notch4 receptor protein was measured by immunohistochemistry method.Results:The angiogenesis density of the skin grafts of the Taohongsiwu decoction was significantly higher than that of the model group,sham operation group and blocker group(P<0.05).The expression level of Notch4 receptor protein in the Taohongsiwu decoction was higher than that in the model group,sham operation group and blocker group(P<0.05).Conclusion:Taohongsiwu decoction can upregulate the expression of Notch4 receptor associated with angiogenesis in tissues and promote the survival of skin grafts.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(81373658)
湖南省教育厅科学研究项目(15C1058)
更新日期/Last Update: 2017-05-01