[1]龚志贤,卢敏,罗凌威,等.跌打通痹膏对兔膝骨关节软骨细胞MMP-1,MMP-3,MMP-13及COL-Ⅱ的 mRNA表达的影响[J].中国中医骨伤科杂志,2016,24(09):1-4，10.
　GONG Zhixian,LU Min,LUO Lingwei,et al.Effect of Dieda Tongbi Emplastrum on Expression of MMP-1, MMP-3,MMP-13 and COL-Ⅱ mRNA in Articular Cartilage Cells of Rabbit with Knee Osteoarthritis[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2016,24(09):1-4，10.
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跌打通痹膏对兔膝骨关节软骨细胞MMP-1,MMP-3,MMP-13及COL-Ⅱ的 mRNA表达的影响
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第24卷
期数:
2016年09期
页码:
1-4，10
栏目:
实验研究
出版日期:
2016-09-01

文章信息/Info

Title:
Effect of Dieda Tongbi Emplastrum on Expression of MMP-1, MMP-3,MMP-13 and COL-Ⅱ mRNA in Articular Cartilage Cells of Rabbit with Knee Osteoarthritis
文章编号:
1005-0205(2016)09-0001-04
作者:
龚志贤1;  卢敏1;  罗凌威2;  范杰2;  邝高艳2;  银丝丝2
1.湖南中医药大学第一附属医院(长沙,410007)
2.湖南中医药大学
Author(s):
GONG Zhixian1;  LU Min1;  LUO Lingwei2;  FAN Jie2;  KUANG Gaoyan2;  YIN Sisi2
1.The First Affiliated Hospital of Hunan University of Traditional Chinese Medcine,Changsha 410007,China;
2.Hunan University of Traditional Chinese Medicine,Changsha 410208,China.
关键词:
膝骨性关节炎;  跌打通痹膏;  基质金属蛋白酶;  Ⅱ型胶原
Keywords:
knee osteoarthritis;  Dieda Tongbi emplastrum;  MMP-1 mRNA;  MMP-3 mRNA;  MMP-13 mRNA;  COL-Ⅱ mRNA
分类号:
R-33
文献标志码:
A
摘要:
目的:研究跌打通痹膏对兔膝骨关节炎关节软骨细胞MMP-1,MMP-3,MMP-13及COL-Ⅱ的mRNA表达的影响,探讨跌打通痹膏干预软骨基质降解的作用机制。方法:新西兰兔60只随机分成空白组、假手术组、模型组、跌打通痹膏组、骨通贴组,每组12只。空白组不做任何处理,模型组、跌打通痹膏组、骨通贴组采用改良Hulth造模法,假手术组打开关节腔后不做处理,直接缝合。正常组、假手术组不予处理,模型组、跌打通痹膏组、骨痛贴组分别用胶带、跌打通痹膏、骨痛贴膏贴敷治疗4周。术后第5周各组随机选择1只兔处死验证造模,进行膝关节软骨形态肉眼观察和切片光镜观察,检测关节软骨中MMP-1,MMP-3,MMP-13及COL-Ⅱ的mRNA表达。结果:1)膝关节软骨形态肉眼观察和切片光镜观察:模型组与空白组有差异,跌打通痹膏组、骨通贴组与模型组比较有差异,跌打通痹膏组与骨通贴组比较无差异。2)MMP-1,MMP-3,MMP-13和COL-Ⅱ的mRNA表达检测:模型组与空白组比较,MMP-1,MMP-3及MMP-13的mRNA表达显著提高,差异有统计学意义(P<0.01),COL-Ⅱ mRNA表达显著降低,差异有统计学意义(P<0.01); 跌打通痹膏组、骨通贴组与模型组比较,MMP-1,MMP-3及MMP-13的mRNA表达显著降低,差异有统计学意义(P<0.01),COL-Ⅱ mRNA表达显著升高,差异有统计学意义(P<0.05); 跌打通痹膏组与骨通贴组比较,MMP-1,MMP-3,MMP-13及COL-Ⅱ的mRNA表达差异无统计学意义(P>0.05)。 结论:1)跌打通痹膏和骨通贴对KOA均有治疗作用,可延缓KOA的病程,且两者间差异无统计学意义。2)跌打通痹膏通过抑制MMP-1,MMP-3及MMP-13的mRNA表达,减缓COL-Ⅱ降解,从而有效保护膝骨关节炎模型兔关节软骨,延缓关节软骨退变。
Abstract:
Objective:To observe the effect of Dieda Tongbi emplastrum on expression of MMP-1,MMP-3,MMP-13 and COL-Ⅱ mRNA in articular cartilage cells of rabbit with knee osteoarthritis,and to explore its mechanism on the degradation of cartilage matrix. Methods:All 60 New Zealand rabbits were randomly divided into blank group,sham-operated group,modeling group,Dieda Tongbi emplastrum group,and gutong emplastrum group(n=12). The knee osteoarthritis model were established by improved Hulth molding method in modeling group,Dieda Tongbi emplastrum group,and Gutong emplastrum,while one rabbits was selected randomly to confirm the successful model 5 weeks after operation. They were treated with tape fixed,Dieda Tongbi emplastrum,and Gutong emplastrum respectively in above threes groups for 4 weeks. The joint cavities were opened without any treatment and sutured directly in the sham-operated group. There was not any treatment in the blank group. Then the knee joint cartilage was observed by eyes and microscopy. The expression of MMP-1,MMP-3,MMP-13 and COL-Ⅱ mRNA in articular cartilage cells were detected. Results:1)There was significant difference between blank group and model group in the knee joint cartilage observation by eyes and microscopy(P<0.05). Compared with model group,Dieda Tongbi emplastrum group and Gutong emplastrum group were significant difference(P<0.05),and there was no significant difference between Dieda Tongbi emplastrum group and Gutong emplastrum group(P>0.05). 2)The expression of MMP-1,MMP-3 and MMP-13 in the model group was increased significantly than blank group(P<0.01),but the COL-ⅡmRNA was decreased significantly(P<0.01). The expression of MMP-1,MMP-3 and MMP-13 in the Dieda Tongbi emplastrum group and Gutong emplastrum group was decreased significantly than model group(P<0.01),but the COL-ⅡmRNA was increased significantly(P<0.05),and there was no significantly difference between Dieda Tongbi emplastrum group and Gutong emplastrum group(P>0.05). Conclusions:Dieda Tongbi emplastrum and Gutong emplastrum are both coequal effective for treating of knee osteoarthritis. Dieda Tongbi emplastrum protects articular cartilage degeneration by inhibiting of MMP-1,MMP-3,and MMP-13 mRNA expression,and reducing the COL-Ⅱdegradation.

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备注/Memo

备注/Memo:
基金项目:湖南省教育厅科研项目-优秀青年项目(13B085)
更新日期/Last Update: 2016-09-15