[1]周广文 向楠△ 沈霖 杨艳萍 帅波 马陈.加味青娥丸对绝经后骨质疏松症患者的骨密度、骨代谢 标志物及血清MGP水平的影响[J].中国中医骨伤科杂志,2016,24(03):4-8.
　ZHOU Guangwen XIANG Nan SHEN Lin YANG Yanping SHUAI Bo MA Chen.Effects of Modified Qing'e Pills on Bone Mineral Density, Bone Metabolic Markers and Serum MGP Level in Postmenopausal Osteoporosis Women[J].Chinese Journal of Traditional Medical Traumatology & Orthopedics,2016,24(03):4-8.
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加味青娥丸对绝经后骨质疏松症患者的骨密度、骨代谢 标志物及血清MGP水平的影响
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《中国中医骨伤科杂志》[ISSN:1005-0205/CN:42-1340/R]

卷:
第24卷
期数:
2016年03期
页码:
4-8
栏目:
临床研究
出版日期:
2016-03-03

文章信息/Info

Title:
Effects of Modified Qing'e Pills on Bone Mineral Density, Bone Metabolic Markers and Serum MGP Level in Postmenopausal Osteoporosis Women
文章编号:
1005-0205(2016)03-0004-05
作者:
周广文1 向楠1△ 沈霖2 杨艳萍2 帅波2 马陈2
1.湖北中医药大学(武汉,430065)
2.华中科技大学同济医学院附属协和医院
△.通信作者 E-mail:xiangnan61@sina.com
Author(s):
ZHOU Guangwen1 XIANG Nan2△ SHEN Lin2 YANG Yanping2 SHUAI Bo2 MA Chen2
1.Hubei University of Traditional Chinese Medicine,Wuhan 430065,China;
2.Union Hosp ital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China.
关键词:
加味青娥丸;  绝经后骨质疏松症;  基质gla蛋白;  骨密度;  骨代谢标志物
Keywords:
modified Qing'e Pills;  postmenopausal osteoporosis;  matrix gla protein;  bone mineral density;  bone metabolism markers
分类号:
R274.39
文献标志码:
A
摘要:
目的:观察加味青娥丸对绝经后骨质疏松症(PMOP)患者基质gla蛋白(MGP)水平、骨密度(BMD)及骨代谢指标的影响。方法:选择PMOP患者108例,随机分为A组(安慰剂对照组)、B组(加味青娥丸低剂量组)及C组(加味青娥丸高剂量组),治疗24周。另取26名BMD正常绝经妇女作为正常组。分别在治疗前与治疗24周后,采用酶联免疫吸附实验(ELISA)测定MGP水平,通过聚合酶链式反应(PCR)检测血清MGP mRNA水平,化学发光检测血清Ⅰ型胶原羧基端肽(β-CTX)、血清Ⅰ型前胶原N-端前肽(P1NP)水平,双能X线吸收法(DEXA)测定腰椎正位、 股骨颈、 Ward's三角和大粗隆的BMD。结果:治疗后C组与B组均能增加血清MGP水平及MGPmRNA的表达(P<0.05或P<0.01),而A组和正常组指标变化不明显(P>0.05)。治疗后C组与B组BMD均有不同程度的提高(P<0.05),C组对股骨颈及Ward's三角的效果更为显著,A组和正常组BMD均下降,差异无统计学意义(P>0.05)。C组能显著降低血清中的骨代谢标志物(PINP,CTX)(P<0.01),而B组仅对β-CTX有降低作用(P<0.05); A组则无明显作用(P>0.05)。结论:加味青娥丸能够提高血清MGP水平及MGP mRNA的表达,增加PMOP的BMD,降低患者骨代谢标志物,并且其效果跟用药剂量正相关。
Abstract:
Objective:To study the effects of Modified Qing'e Pills on serum MGP level,bone mineral density and bone metabolic markers in women with postmenopausal osteoporosis.Methods:108 postmenopausal osteoporosis patients were randomly divided into group(Placebo control group),B group(Modified Qing'e Pills lower dose group)and C group(Modified Qing'e Pills higher dose group).All the patients in three groups received treatment for 24 weeks.Another 26 women with normal BMD were assigned to normal group.Before and after the treatment for 24 weeks,serum MGP was measured by enzyme-linked immunosorbent assay(ELISA)while MGP mRNA was measured by Fluorescent real-time quantitative polymerase chain reaction(PCR).The serum levels of β-CTX、P1NP were measured by electrochemiluminescence(ECL).BMD was analyzed by dual energy X-ray absorptiometry(DXA).Results:After 24 weeks treatment,MGP and MGP mRNA were improved obviously in C group and B group(P<0.05 or P<0.01),while no obvious changes of MGP and MGP mRNA in normal group and A group(P>0.05).The BMD of C group and B group were all improved significantly(P<0.05),and the BMD of C group in femoral neck and Ward's triangle increased more significantly(P<0.01).The BMD of normal group and A group were decreased.All the bone metabolism markers of C group reduced obviously(P<0.05),whereas only the bone absorption marker β-CTX decreased in B group(P<0.05).The normal group and A group had no obvious clinical effects(P>0.05)Conclusion:Modified Qing'e Pills can benefit postmenopausal osteoporosis patients through improving the serum MGP levels and MGP mRNA expression,increasing bone mineral density and inhibiting bone metabolism.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金项目(81473492)
更新日期/Last Update: 2016-03-15